Journal
PLANT CELL AND ENVIRONMENT
Volume 34, Issue 12, Pages 2024-2037Publisher
WILEY
DOI: 10.1111/j.1365-3040.2011.02396.x
Keywords
amino acid metabolism; ascorbate-glutathione cycle; carbon metabolism; hydroponics; mass spectrometry; nitrate; proteome analysis; redox regulation; two-dimensional gel electrophoresis
Categories
Funding
- Danish Research Council for Technology and Production Sciences (LIP)
- Danish Research Council for Natural Sciences (ENV)
- Danish Strategic Research Council
Ask authors/readers for more resources
Cereals are major crops worldwide, and improvement of their nitrogen use efficiency is a crucial challenge. In this study proteins responding to N supply in barley roots and shoots were analysed using a proteomics approach, to provide insight into mechanisms of N uptake and assimilation. Control plants grown hydroponically for 33 d with 5 mm nitrate, plants grown under N deficiency (0.5 mm nitrate, 33 d) or short-term N starvation (28 d with 5 mm nitrate followed by 5 d with no N source) were compared. N deficiency caused changes in C and N metabolism and ascorbate-glutathione cycle enzymes in shoots and roots. N starvation altered proteins of amino acid metabolism in roots. Both treatments caused proteome changes in roots that could affect growth. Shoots of plants grown with ammonium as N source (28 d with 5 mm nitrate followed by 5 d with 5 mm ammonium) showed responses similar to N deficient shoots, characterized by turnover of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and increases in proteins of the chloroplastic transcription and translation machinery. Identified proteins in 67 and 49 varying spots in roots and shoots respectively, corresponded to 62 functions and over 80 gene products, considerably advancing knowledge of N responses in barley.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available