Article
Biochemistry & Molecular Biology
Miao Lan, Xianan Liu, Erfang Kang, Ying Fu, Lei Zhu
Summary: Microtubulin dynamics and organization are crucial for the development and morphogenesis of plant cells. The motor protein kinesins, which are based on microtubules, are primarily responsible for the transport of organelles and vesicles, while some also regulate microtubule organization. ARK family, a plant-specific motor protein subfamily, has three members in Arabidopsis thaliana (ARK1, ARK2, and ARK3). It has been shown that ARK2 is involved in root epidermal cell morphogenesis, but its association with microtubules and the underlying mechanism need further exploration.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Dong Jin Mun, Bon Seong Goo, Bo Kyoung Suh, Ji-Ho Hong, Youngsik Woo, Soo Jeong Kim, Seunghyun Kim, Su Been Lee, Yubin Won, Jin Yeong Yoo, Eunbyul Cho, Eun Jin Jang, Truong Thi My Nhung, Hong Minh Triet, Hongyul An, Haeryun Lee, Minh Dang Nguyen, Seung-Yeol Park, Seung Tae Baek, Sang Ki Park
Summary: In this study, the Gcap14-Ndel1 complex is identified as a fundamental regulator of cytoskeletal remodeling during neurodevelopment, including neuronal process elongation and migration. Deficiency of Gcap14 leads to impaired cortical lamination and defective neuronal migration. The interaction between Gcap14 and Ndel1 effectively corrects the downregulation of microtubule dynamics and the defects in neuronal migration caused by Gcap14 deficiency.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Klara Prikrylova Konecna, Agata Kilar, Petra Kovacikova, Jiri Fajkus, Eva Sykorova, Miloslava Fojtova
Summary: This study investigates the interaction between ARM and telomerase and explores the cellular functions of ARM. The results show that ARM is involved in crucial cellular processes and has a dominant effect. Additionally, ARM is implicated in circadian rhythms and responses to drugs and stimuli.
Article
Multidisciplinary Sciences
Wangxi Luo, Vladimir Demidov, Qi Shen, Hugo Girao, Manas Chakraborty, Aleksandr Maiorov, Fazly I. Ataullakhanov, Chenxiang Lin, Helder Maiato, Ekaterina L. Grishchuk
Summary: CLASPs are universal stabilizers of microtubule dynamics, and this study reveals that clusters of human CLASP2 form a load-bearing bond with terminal non-GTP tubulins at the stabilized microtubule tip. This activity relies on the TOG2 domain of CLASP2, which releases its high-affinity bond with non-GTP tubulins when they convert into polymerization-competent GTP-tubulins. The recognition of nucleotide-specific tubulin conformation by CLASP2 and its ability to stabilize non-GTP tubulins contribute to the suppression of catastrophe at freely assembling microtubule ends and the promotion of persistent tubulin assembly at load-bearing tethered ends.
Article
Multidisciplinary Sciences
Maxim Igaev, Helmut Grubmuller
Summary: This study uses atomistic simulations to investigate the bending and relaxation behavior of microtubule tips, revealing the dominance of viscoelastic dynamics. The posthydrolysis microtubule tip is unable to elongate due to higher activation energy barriers for straight lattice formation. This research provides an information-driven mechanism for the elastic energy conversion and release by microtubule tips.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Ben M. Smith, Pamela J. E. Rowling, Christopher M. Dobson, Laura S. Itzhaki
Summary: The study investigates the interaction between beta-catenin and TCF7L2, revealing the kinetic characteristics through a fluorescence reporter system and mutagenesis analysis. The results suggest a complex two-site avidity mechanism of binding, with the involvement of transient contacts and alternative dissociation pathways. The study provides insights into the molecular recognition of a long intrinsically disordered region with an elongated repeat-protein surface, involving parallel routes and sequential steps.
Article
Cell Biology
Veronica Farmer, Goker Arpa, Sarah L. Hall, Marija Zanic
Summary: The GTP-tubulin cap is believed to protect microtubules against catastrophe, but microtubules polymerized with XMAP215 display increased catastrophe frequency despite larger GTP-cap size. Structural perturbations induced by XMAP215 may override the protective effects of the GTP-cap.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Junhyun Park, Yi Xie, Kenneth G. Miller, Pietro De Camilli, Shaul Yogev
Summary: Axonal transport is crucial for neuronal function. In this study, EBP-1 was found to regulate the association between kinesin-3/KIF1A/UNC-104 and DCVs, leading to efficient axonal delivery of specific cargo. EBP-1 functions at the trans Golgi by directing microtubule growth and interacting with KIF1A. These findings provide insights into the mechanisms of specific kinesin-3 cargo delivery to the axon.
Article
Plant Sciences
Jin Hoon Jang, Hae Seong Seo, Ok Ran Lee
Summary: Among the three subfamilies of patatin-related phospholipase A (pPLA) group of genes, overexpression of pPLAIII gamma leads to reduced longitudinally cell expansion patterns. The altered phenotype is linked with transcriptional modulation of microtubule-associated genes and ethylene biosynthesis.
Article
Cell Biology
Xiaoyu Song, Fengrui Yang, Tongtong Yang, Yong Wang, Mingrui Ding, Linge Li, Panpan Xu, Shuaiyu Liu, Ming Dai, Changbiao Chi, Shengqi Xiang, Chao Xu, Dong Li, Zhikai Wang, Lin Li, Donald L. Hill, Chuanhai Fu, Kai Yuan, Pilong Li, Jianye Zang, Zhonghuai Hou, Kai Jiang, Yunyu Shi, Xing Liu, Xuebiao Yao
Summary: EB1 proteins form molecular condensates through liquid-liquid phase separation (LLPS) to constitute the microtubule plus-end machinery, which is crucial for cellular dynamics and organelle movements.
NATURE CELL BIOLOGY
(2023)
Article
Plant Sciences
Xiaolei Liu, Xiaoyun Tan
Summary: In this study, the Arabidopsis MOR1 gene was found to play important roles in filament functions and anther dehiscence. The mor1-1 mutant exhibited an indehiscence phenotype at higher temperature, with disrupted CMT organization and plasma membrane homeostasis in filament cells. These results indicate that MOR1 mediated CMT organization is crucial for clathrin-mediated endocytosis in filament cells, and critical for anther dehiscence under thermosensitivity.
PLANT GROWTH REGULATION
(2022)
Review
Biochemistry & Molecular Biology
Yutao Huang, Zijian Jiang, Xiangyu Gao, Peng Luo, Xiaofan Jiang
Summary: Armadillo repeat-containing proteins (ARMCs) are widely distributed in eukaryotes and play important roles in various processes. They share similar domain structures and play critical roles in different diseases.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Cell Biology
Kun Fan, Xiaojian Ni, Sheng Shen, Zijun Gong, Jiwen Wang, Yanlei Xin, Bohao Zheng, Wentao Sun, Han Liu, Tao Suo, Xiaoling Ni, Houbao Liu
Summary: Gallbladder cancer is a common malignant tumor in the biliary tract, characterized by high metastatic potential and poor prognosis. This study revealed that K9 acetylation of Stathmin1 is associated with tumor metastasis in gallbladder cancer, suggesting it as a potential target for therapy.
CELL DEATH DISCOVERY
(2022)
Article
Cell Biology
Yunfeng Hu, Qiu Xie, Xiang Wu, Weizhen Liu, DongFang Li, Chen Li, WangXing Zhao, LinLin Chen, Zihui Zheng, GuangMing Li, Jun Guo
Summary: Clip170 tension is closely associated with breast cancer invasion and migration. It can be regulated by alpha-tubulin detyrosination-induced MT disassembly and increased by Clip170 phosphorylation induced by RSK. Enhanced Clip170 tension promotes tumor cell invasion and metastasis and is correlated with the distribution variation of other proteins. Tension of Clip170 could be a potential drug target for breast cancer therapy.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Stefano Cucuzza, Peter Guentert, Andreas Plueckthun, Oliver Zerbe
Summary: An automated iterative procedure for backbone protein structure refinements using a limited amount of backbone amide PCSs is presented in this study, which can be particularly useful when approximate folds are known from other techniques and can produce reliable indicators through experimental PCSs.
JOURNAL OF BIOMOLECULAR NMR
(2021)
Article
Plant Sciences
Ryan C. Eng, Laryssa S. Halat, Samuel J. Livingston, Tatsuya Sakai, Hiroyasu Motose, Geoffrey O. Wasteneys
PLANT AND CELL PHYSIOLOGY
(2017)
Review
Cell Biology
Andrei Smertenko, Farhah Assaad, Frantisek Baluska, Magdalena Bezanilla, Henrik Buschmann, Georgia Drakakaki, Marie-Theres Hauser, Marcel Janson, Yoshinobu Mineyuki, Ian Moore, Sabine Mueller, Takashi Murata, Marisa S. Otegui, Emmanuel Panteris, Carolyn Rasmussen, Anne-Catherine Schmit, Jozef Samaj, Lacey Samuels, L. Andrew Staehelin, Daniel Van Damme, Geoffrey Wasteneys, Viktor Zarsky
TRENDS IN CELL BIOLOGY
(2017)
Review
Plant Sciences
Ryan Christopher Eng, Arun Sampathkumar
CURRENT OPINION IN PLANT BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Yuan Ruan, Laryssa S. Halat, Deirdre Khan, Sylwia Jancowski, Chris Ambrose, Mark F. Belmonte, Geoffrey O. Wasteneys
Article
Plant Sciences
Zhongshou Wu, Meng Li, Oliver Xiaoou Dong, Shitou Xia, Wanwan Liang, Yongkang Bao, Geoffrey Wasteneys, Xin Li
Article
Developmental Biology
Arun Sampathkumar, Alexis Peaucelle, Miki Fujita, Christoph Schuster, Staffan Persson, Geoffrey O. Wasteneys, Elliot M. Meyerowitz
Article
Plant Sciences
Patrick T. Martone, Kyra Janot, Miki Fujita, Geoffrey Wasteneys, Katia Ruel, Jean-Paul Joseleau, Jose M. Estevez
Editorial Material
Biochemistry & Molecular Biology
Geoffrey O. Wasteneys
Editorial Material
Biochemistry & Molecular Biology
Ryan Christopher Eng, Arun Sampathkumar
Article
Plant Sciences
Laryssa Halat, Katherine Gyte, Geoffrey Wasteneys
Article
Multidisciplinary Sciences
Jacqueline Nowak, Ryan Christopher Eng, Timon Matz, Matti Waack, Staffan Persson, Arun Sampathkumar, Zoran Nikoloski
Summary: While cell shape is crucial for the function and development of organisms, versatile frameworks for cell shape quantification, comparison, and classification remain underdeveloped. Here, the authors use a network-based framework for Arabidopsis leaf epidermal cell shape characterization and classification.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Ryan C. Eng, Rene Schneider, Timon W. Matz, Ross Carter, David W. Ehrhardt, Henrik Joensson, Zoran Nikoloski, Arun Sampathkumar
Summary: This study investigates how mechanical stress influences cell and tissue form at different length scales in Arabidopsis thaliana cotyledon tissue. The researchers find that microtubule organization in pavement cells is mediated by chemical signals and cell-shape-derived mechanical stress during early development stages and regulated by KATANIN and CLASP proteins. However, the impact of these proteins on tissue-scale mechanical stress varies in the epidermis of cotyledons. Overall, the study shows that cotyledon form regulation is separate from the control of pavement cell shape mediated by microtubule organization under subcellular mechanical stress.
Review
Plant Sciences
Laryssa Sophia Halat, Breanne Bali, Geoffrey Wasteneys
Summary: The transition from cell division to differentiation in primary roots is influenced by gradients of phytohormones and the reorganization of microtubules. The microtubule-associated protein CLASP acts as an important intermediate between hormone signaling and microtubule organization. New tools and methods, such as hormone sensors and computer modeling, allow researchers to accurately visualize the belowground growth dynamics of plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Marcus Woodley, Adam Mulvihill, Miki Fujita, Geoffrey O. Wasteneys