Journal
PLANT CELL
Volume 24, Issue 4, Pages 1316-1326Publisher
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.112.096362
Keywords
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Funding
- Biotechnology and Biological Sciences Research Council [BB/D016223/1] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BB/D016223/1, BB/J017582/1] Funding Source: researchfish
- BBSRC [BB/J017582/1, BB/D016223/1] Funding Source: UKRI
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Transient gene expression, in plant protoplasts or specific plant tissues, is a key technique in plant molecular cell biology, aimed at exploring gene products and their modifications to examine functional subdomains, their interactions with other biomolecules, and their subcellular localization. Here, we highlight some of the major advantages and potential pitfalls of the most commonly used transient gene expression models and illustrate how ectopic expression and the use of dominant mutants can provide insights into protein function.
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