4.4 Article

A new phenotyping technique for screening for drought tolerance in lentil (Lens culinaris Medik.)

Journal

PLANT BREEDING
Volume 132, Issue 2, Pages 185-190

Publisher

WILEY-BLACKWELL
DOI: 10.1111/pbr.12033

Keywords

drought tolerance; hydroponic technique; lentil

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Screening for drought tolerance is severely handicapped by the lack of a simple and reliable phenotyping technique. The objective of this study was to develop a new screening technique based on seedling survivability, drought tolerance score, root and shoot length, and fresh and dry weight of roots and shoots of lentil plants exposed to drought under hydroponic conditions. Its effectiveness was compared with two soil culture techniques. The hydroponic technique involved removing 15-day-old seedlings of 80 genotypes from the nutrient solution and exposing them to air for 5h daily for 6days. Three genotypes received from ICARDA, ILL-10700', ILL-10823' and FLIP-96-51', showed maximum seedling survivability and minimum reduction in the growth parameters with a drought score of 0.00.2 indicating higher tolerance to drought stress, while Indian genotypes JL-3', E-153' and VL-507' showed no seedling survivability and maximum reduction in growth parameters with a drought score of 4.0 indicating low drought tolerance. The results suggest that this new phenotyping technique is effective, rapid and easy for screening a large number of genotypes.

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