Journal
PLANT BIOTECHNOLOGY REPORTS
Volume 7, Issue 3, Pages 277-286Publisher
SPRINGER
DOI: 10.1007/s11816-012-0261-0
Keywords
Habanero pepper; Somatic embryos; Histology; Scanning electron microscopy
Funding
- CONACYT
- FOMIX
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Somatic embryo-like structures were produced from the hypocotyls of aseptic plants of Capsicum chinense. Different concentrations of 2,4-dichlorophenoxyacetic acid (0, 4.5, 9.05 mu M), several exposure times of the explant to this auxin (15, 30, 45, 60 days) and the development of somatic embryos cultured in a solid and/or liquid medium were evaluated. As a result, a novel system of regeneration via direct somatic embryogenesis in liquid medium was established, with an efficiency of 1.77 x 10(4) somatic embryos per liter of medium. Critical stages of embryogenesis, including cellular acquisition of morphogenetic competence, suspensor formation, and development and maturation of somatic embryos, were identified by histological analysis and scanning electron microscopy. Our results show a promising new outlook on the in vitro regeneration of this species. Contrary to what has been reported to date for the Capsicum genus, it is a species of plants with higher embryogenic potential in vitro.
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