Article
Plant Sciences
Chunyao Tian, Huige Quan, Ruiping Jiang, Qiaomu Zheng, Sipei Huang, Guodong Tan, Chaoyue Yan, Jiayu Zhou, Hai Liao
Summary: Due to global climate change, drought has become a major threat to plant growth and agricultural productivity. In this study, potential candidate genes controlling isoprenoid biosynthesis were identified from the Cassia tora genome, and transgenic plants expressing these genes exhibited different performances in drought tolerance and growth.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Carlota Roca, Yunuen Avalos-Padilla, Beatriz Prieto-Simon, Valentin Iglesias, Miriam Ramirez, Santiago Imperial, Xavier Fernandez-Busquets
Summary: The methyl erythritol phosphate (MEP) pathway is crucial for malaria parasites and human pathogenic bacteria. A DNA aptamer (D10) has been developed to target the enzyme DXR in this pathway, showing high selectivity and specificity for the detection of bacterial and parasitized cells. This study suggests the potential of DNA aptamers as ligands for future therapeutic and diagnostic applications.
Article
Genetics & Heredity
Yueya Zhang, Haifeng Yan, Yuan Li, Yuping Xiong, Meiyun Niu, Xinhua Zhang, Jaime A. Teixeira da Silva, Guohua Ma
Summary: The essential oil derived from sandalwood heartwood contains highly valued sesquiterpenoids that are widely used in the fragrance industry. SaDXR, an enzyme in the MEP pathway, plays a crucial role in biosynthesis of photosynthetic pigments and sandalwood-specific sesquiterpenoids. SaDXR expression is influenced by light exposure, and overexpression of SaDXR in transgenic Arabidopsis plants leads to increased chlorophylls and carotenoids levels.
Article
Chemistry, Medicinal
Xian Hui, Bo-Rong Zhu, Long-Long Wu, Wen-Yun Gao, Yi-Ming Li, Qi Jia, Heng Li
Summary: The study found that five proanthocyanidins demonstrated inhibitory effects on DXR, with procyanid B2 and procyanid C1 showing relatively weaker effects, while the other three compounds exhibited inhibitory effects slightly weaker than procyanid C1. The structure-activity relationship of these compounds against DXR was discussed based on initial characterization.
NATURAL PRODUCT COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Chengge Zhang, Huanhuan Liu, Shan Hu, Yaxian Zong, Hui Xia, Huogen Li
Summary: By analyzing differentially expressed genes in two Liriodendron species, 130 genes related to terpenoid synthesis were identified, with the monoterpenoid biosynthesis pathway being the most significant. The LtuDXR gene was cloned and its role in volatile terpenoid synthesis was studied, leading to an increased understanding of the molecular mechanism behind terpenoid biosynthesis regulation in Liriodendron.
Article
Nutrition & Dietetics
Minhee Lee, Dakyung Kim, Hyunji Kim, Sukyung Jo, Ok-Kyung Kim, Jeongmin Lee
Summary: The study demonstrated that Glycine max (L.) Merr. fermented using Lactobacillus delbrueckii ssp. delbrueckii Rosell-187 (Gastro-AD (R)) can alleviate ethanol/HCl-induced gastric damage, with gastro-protective effects such as reducing gastric acid secretion, increasing prostaglandin E2 levels, and decreasing inflammatory factors levels.
Article
Biochemistry & Molecular Biology
Gustavo dos Santos Cotrim, Deivid Metzker da Silva, Jose Perez da Graca, Adilson de Oliveira Junior, Cesar de Castro, Guilherme Juliao Zocolo, Luciola Santos Lannes, Clara Beatriz Hoffmann-Campo
Summary: Potassium availability significantly impacts soybean metabolism, leading to changes in the composition of various metabolites in different tissues. The deficiency of potassium in soil can cause the accumulation of certain elements in soybean leaves, while potassium fertilization promotes the synthesis of carbohydrates, galactolipids, and flavonol glycosides in leaves and pod valves. In contrast, potassium-deficient pod tissues show increased contents of amino acids, oligosaccharides, benzoic acid derivatives, and isoflavones. The results also suggest that L-asparagine can be used as a biomarker for potassium deficiency in soybean plants.
Article
Biochemistry & Molecular Biology
Eguono W. Omagamre, Yeganeh Mansourian, Diamond Liles, Tigist Tolosa, Simon A. Zebelo, Joseph S. Pitula
Summary: This study investigated the impact of PFBA on soybean plants and found that it stimulated and inhibited plant growth. It also affected oxidative stress response and transcriptomic activity. The circadian rhythm and ethylene signaling pathway were found to be regulated in response to PFBA exposure. These findings suggest that PFBA induces stress in soybean plants, and the observed stimulation at 100 ng/L may represent an overcompensation response via the circadian rhythm pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xuanbo Zhong, Wei Hong, Yue Shu, Jianfei Li, Lulu Liu, Xiaoyang Chen, Faisal Islam, Weijun Zhou, Guixiang Tang
Summary: This study reported the characterization and function of GmHdz4, a HD-Zip I gene, in soybean response to drought stress. GmHdz4 was found to localize in the plant nucleus and display transcriptional activation activity, and its expression was induced by PEG-simulated drought stress. Editing of GmHdz4 using the CRISPR/Cas9 system was shown to enhance drought tolerance in soybean. These findings provide new insights for future molecular breeding research in soybean.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Samantha McConaughy, Keenan Amundsen, Qijian Song, Vince Pantalone, David Hyten
Summary: Recombination allows for the exchange of genetic material between two parents, which can be used by plant breeders to improve cultivars. Recombination hotspots, which are clustered regions of crossovers, mainly occur in certain areas of the genome. Understanding the distribution and sequence motifs associated with these hotspots can help breeders effectively utilize recombination in breeding.
G3-GENES GENOMES GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Aurore Dreneau, Fanny S. Krebs, Mathilde Munier, Chheng Ngov, Denis Tritsch, Didier Lievremont, Michel Rohmer, Catherine Grosdemange-Billiard
Summary: Three alpha,alpha-difluorophosphonate derivatives of fosmidomycin were synthesized and evaluated for their antimicrobial activity against Escherichia coli, with two of them showing significantly enhanced inhibitory effects compared to the reference compound fosmidomycin.
Article
Biochemistry & Molecular Biology
Luis de Luna-Valdez, Marel Chenge-Espinosa, Arihel Hernandez-Munoz, Elizabeth Cordoba, Gamaliel Lopez-Leal, Santiago Castillo-Ramirez, Patricia Leon
Summary: The DXS3 subfamily plays a crucial role in the MEP pathway in generating plastidial isoprenoids and shows unique evolution and functionality compared to DXS1 and DXS2 subfamilies. DXS3 proteins lack functional activity in Arabidopsis, Maize, and Rice, and display distinct sub-organellar chloroplast localization. DXS3 coexpresses with genes related to post-embryonic development and reproduction, indicating a different role compared to DXS1 and DXS2 proteins in primary metabolism and isoprenoid synthesis.
Article
Agronomy
Ciro Antonio Rosolem, Thiago Barbosa Batista, Patricia Pereira Dias, Laudelino Vieira da Motta Neto, Juliano Carlos Calonego
Summary: The joint application of phosphorus (P) and ammonium (N-NH4+) has been shown to increase maize root proliferation and P acquisition in alkaline soils. This study aimed to assess the effect of the joint application of P and N-NH4+ on soybean root growth and P acquisition. The results showed that P fertilization increased the soil P content, decreased the rhizosphere pH, and resulted in increased soybean P uptake and use efficiency.
Article
Biochemistry & Molecular Biology
Zhengjie Chen, Wenjuan Zhong, Yonghang Zhou, Peicheng Ji, Yonglu Wan, Shengjia Shi, Zehu Yang, Yiyun Gong, Fangsheng Mu, Siwei Chen
Summary: This study compared the seed quality differences between vegetable soybean and grain soybean through metabolome and transcriptome analysis, and identified changes in metabolic pathways and gene expression, providing a better understanding of seed development and breeding improvements in vegetable soybean.
Article
Fisheries
Wei Wang, Furong Zhao, Qian Li, Yonghua Lin, Chen Liang, Jianming Chen
Summary: The infectious diseases caused by Vibrio species present a major challenge in the shrimp aquaculture industry, calling for the search for new targets and antibiotics to address multi-drug resistance. DXR is critical for the growth of V. vulnificus and could serve as a potential target for anti-Vibrio agents screening.