Journal
PLANT BIOTECHNOLOGY REPORTS
Volume 4, Issue 1, Pages 49-52Publisher
SPRINGER
DOI: 10.1007/s11816-009-0117-4
Keywords
Chelex 100; DNA extraction method; Plant material; PCR; Transgenes
Funding
- BioGreen 21 Program [20070301034020]
- Rural Development Administration, Republic of Korea
Ask authors/readers for more resources
A DNA extraction method using Chelex 100 is widely used for bacteria, Chlamydomonas, and animal cell lines, but only rarely for plant materials due to the need for additional time-consuming and tedious steps. We have modified the Chelex 100 protocol and successfully developed a rapid and simple method of DNA extraction for efficient PCR-based detection of transgenes from a variety of transgenic plant and algal species. Our protocol consists of homogenizing plant tissue with a pestle, boiling the homogenized tissue in a microfuge tube with 5% Chelex 100 for 5 min, and centrifuging the boiled mixture. The supernatant, which is used for PCR analysis, was able to successfully amplify transgenes in transgenic tobacco, tomato, potato, Arabidopsis, rice, strawberry, Spirodela polyrhiza, Chlamydomonas, and Porphyra tenera. The entire DNA extraction procedure requires < 15 min and is therefore comparable to that used for bacteria, Chlamydomonas, and animal cell lines.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available