Journal
PLANT BIOTECHNOLOGY REPORTS
Volume 2, Issue 3, Pages 207-212Publisher
SPRINGER
DOI: 10.1007/s11816-008-0063-6
Keywords
in vitro culture; light intensity; photoperiod; stomatal development; relative water content
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Plantlets of Alocasia amazonica regenerated under a photon flux density (PFD) of 15 or 30 mu mol m(-2) s(-1) showed better growth and development than those grown under higher PFDs. While chlorophyll a and chlorophyll b decreased, the number of stomata increased with increasing PFD. Photoperiods also affected plantlet growth and stomatal development. Highest growth was observed for the short photoperiod (8/16 h) and for equinoctial (12/12 h) light and dark periods. Very few stomata developed in the leaves of plantlets grown under a short photoperiod (8/16 h) and the number of stomata increased with increasing light period. In conclusion, both light intensity and photoperiod independently affect growth of A. amazonica and development of stomata, depending on the intensity and duration of light treatment.
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