Article
Virology
Daria V. Voronina, Dmitry Shcheblyakov, Irina A. Favorskaya, Ilias B. Esmagambetov, Alina S. Dzharullaeva, Amir Tukhvatulin, Olga Zubkova, Olga Popova, Vladislav Y. Kan, Alina S. Bandelyuk, Maxim M. Shmarov, Denis Y. Logunov, Boris S. Naroditskiy, Aleksandr L. Gintsburg
Summary: This study modified two potent single-domain antibodies to enhance their antiviral activity against multiple influenza subtypes, demonstrating effective prophylaxis and therapy in mice.
Article
Plant Sciences
Maofeng Chai, Indira Queralta Castillo, Annika Sonntag, Shixing Wang, Zhili Zhao, Wei Liu, Juan Du, Hongli Xie, Fuqi Liao, Jianfei Yun, Qingzhen Jiang, Juan Sun, Isabel Molina, Zeng-Yu Wang
Summary: This study highlights the importance of KCS12 in controlling the production of very long chain lipid species in the seed coat, ultimately affecting the physical dormancy of seeds. The expression of KCS12 in the seed coat is directly regulated by the transcription factor KNOX4, defining a molecular mechanism by which KNOX4 and KCS12 work together to control seed coat formation and physical dormancy.
Article
Multidisciplinary Sciences
Xiaochao Chen, Dana R. R. MacGregor, Francesca L. L. Stefanato, Naichao Zhang, Thiago Barros-Galvao, Steven Penfield
Summary: Mother plants play a crucial role in determining the dispersal characteristics of their progeny by controlling seed dormancy. The study shows that the VEL3 histone deacetylase complex establishes an epigenetic state in the maternal plant, which regulates progeny seed dormancy. This mechanism involves the association of VEL3 with a histone deacetylase complex, preferential attachment to pericentromeric chromatin, and repression of specific genes to control seed behavior.
NATURE COMMUNICATIONS
(2023)
Article
Agriculture, Multidisciplinary
Yumin Zhang, Dan Li, Lynnette M. A. Dirk, A. Bruce Downie, Tianyong Zhao
Summary: ZmAGA1 overexpression in Arabidopsis reduces RFOs and galactinol content in seeds, leading to increased seed germination percentage under stress conditions, but decreased seed aging tolerance. Manipulation of ZmAGA1 expression can potentially regulate maize seed germination and aging tolerance simultaneously.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Angela Donato Maia Malaquias, Livia Erika Carlos Marques, Soraya S. Pereira, Cleberson de Freitas Fernandes, Andrea Queiroz Maranhao, Rodrigo G. Stabeli, Eridan Orlando Pereira Tramontina Florean, Maria Izabel Florindo Guedes, Carla Freire Celedonio Fernandes
Summary: VHHs are small functional immunoglobulins that exhibit high tissue penetration and low immunogenicity. They provide superior antigen recognition compared to human antibodies, along with better solubility and stability. Plant-based expression systems offer versatility, cost-effectiveness, safety, and efficiency in producing VHH-related constructs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Plant Sciences
Adam Chin-Fatt, Rima Menassa
Summary: Chimeric fusion proteins consisting of VHH and Fc fragments show increased accumulation when targeted to the chloroplast thylakoid lumen, indicating the potential of this compartment for stable folding of disulfide-containing recombinant proteins.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Mireille Cambert, Adeline Berger, Christine Salle, Stephanie Esling, Delphine Charif, Tudel Cadoret, Marie-Christine Ralet, Helen M. North, Corinne Rondeau-Mouro
Summary: The seeds of Arabidopsis thaliana produce a mucilage layer when imbibed, comprising an outer and inner layer. By studying 19 natural accessions, researchers have generated datasets for further exploration of mucilage polysaccharides and their adaptive traits. This data will serve as a valuable resource for genetic, biochemical, structural, and functional analyses.
Article
Agronomy
Langlang Ma, Chen Wang, Yu Hu, Wei Dai, Zhenjuan Liang, Chaoying Zou, Guangtang Pan, Thomas Lubberstedt, Yaou Shen
Summary: The key gene MADS26 affecting maize seed germination was identified through GWAS and transcriptomics. The study revealed variations within MADS26 that regulate seed germination traits and showed that overexpressing MADS26 improves seed germination in Arabidopsis. Understanding the genetic control of seed germination ability in maize is important for improving maize yield.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Xiangling Liu, Na Li, Aoyu Chen, Noor Saleem, Qingli Jia, Cuizhu Zhao, Wenqiang Li, Meng Zhang
Summary: The transcription factor AINTEGUMENTA-like6 functions as a downstream regulator of the master regulator FUSCA3 in seed dormancy and lipid metabolism. AIL6 is activated by FUS3 and has a role in modulating fatty acid compositions in seeds. Over-expression of AIL6 leads to low seed germination rates and alterations in lipid metabolism, which can be released by the della quintuple mutant.
Article
Agricultural Engineering
Sayedehsaba Bilgrami, Liezhao Liu, Sara Farokhzadeh, Ahmad Sobhani Najafabadi, Hadi Darzi Ramandi, Najmeh Nasiri, Ibrahim Darwish
Summary: This study collected QTL information from multiple populations and identified overlapping regions associated with seed quality traits in Brassica napus, showing potential for improving multiple desirable traits simultaneously. Enrichment regions with more QTLs in some traits were found, providing clues for discovering varied areas of improvement. Additionally, candidate genes in overlapping regions were analyzed, revealing key known genes, novel genes, and genes with unknown functions involved in different biological processes of quality traits.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Zhiyang Yuan, Kai Fan, Yuntong Wang, Li Tian, Chaopu Zhang, Wenqiang Sun, Hanzi He, Sibin Yu
Summary: This study cloned and characterized the rice seed storability-related gene OsGH3-2, which was shown to affect seed storability by regulating the accumulation level of abscisic acid (ABA). Transgenic, knockout, and knockdown experiments revealed that OsGH3-2 acted as a negative regulator of seed storability by modulating genes related to the ABA pathway and late embryogenesis-abundant proteins.
Article
Plant Sciences
Myeong Hun Yeon, Chan-Ho Park, Ye Eun Lee, Jeehee Roh, Seong-Ki Kim
Summary: Introducing the Arabidopsis Cytochrome P450 85A2 gene into Arabidopsis seeds can significantly enhance plant growth and development, leading to increased seed yields and improved plant traits.
JOURNAL OF PLANT BIOLOGY
(2022)
Article
Plant Sciences
Bing Bai, Niels van der Horst, Jan H. Cordewener, Antoine H. P. America, Harm Nijveen, Leonie Bentsink
Summary: The study investigated the correlation between total mRNA, polysome-associated mRNA, and protein levels during seed germination, finding a low direct correlation between polysomal mRNA and protein abundance. Genes with delayed protein changes were classified, specific motifs in these genes were identified, and mRNA and protein stability as well as mRNA length were found to be important predictors for changes in protein abundance. In conclusion, polysome association and/or dissociation predicts future changes in protein abundance in germinating seeds.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Huabin Liu, Qiong Luo, Chao Tan, Jia Song, Tan Zhang, Shuzhen Men
Summary: Auxin plays an important role in the coordinated development of the embryo, endosperm, and seed coat during seed development. In this study, the distribution pattern and function of auxin during Arabidopsis seed development were investigated. It was found that auxin response signals exhibited a dynamic distribution pattern, with strong signals observed at specific locations in the seed coat. Auxin biosynthesis genes and auxin carriers were identified as contributing factors to the accumulation and polar distribution of auxin in the seed coat. Furthermore, the accumulation of auxin in the seed coat was found to regulate seed size. These findings provide valuable insights and tools for improving crop yields.
Article
Plant Sciences
Jia-Xing Guo, Ru-Feng Song, Kai-Kai Lu, Yu Zhang, Hui-Hui Chen, Jia-Xin Zuo, Ting-Ting Li, Xue-Feng Li, Wen-Cheng Liu
Summary: The C-type cyclin protein CycC1;1 interacts with ABI5 to downregulate ABA-responsive genes and ABI5 itself, thereby inhibiting ABA signaling during seed germination.
Review
Biochemistry & Molecular Biology
Vikram Virdi, Paloma Juarez, Veronique Boudolf, Ann Depicker
CELLULAR AND MOLECULAR LIFE SCIENCES
(2016)
Article
Biotechnology & Applied Microbiology
Thomas De Meyer, Bram Laukens, Jonah Nolf, Els Van Lerberge, Riet De Rycke, Ans De Beuckelaer, Sylvie De Buck, Nico Callewaert, Ann Depicker
PLANT BIOTECHNOLOGY JOURNAL
(2015)
Article
Biochemical Research Methods
Abel Hernandez-Velazquez, Alina Lopez-Quesada, Yanaysi Ceballo-Camara, Gleysin Cabrera-Herrera, Kenia Tiel-Gonzalez, Liliana Mirabal-Ortega, Marlene Perez-Martinez, Rosabel Perez-Castillo, Yamilka Rosabal-Ayan, Osmani Ramos-Gonzalez, Gil Enriquez-Obregon, Ann Depicker, Merardo Pujol-Ferrer
TRANSGENIC RESEARCH
(2015)
Review
Biotechnology & Applied Microbiology
Edward Topp, Rebecca Irwin, Tim McAllister, Martin Lessard, Jussi J. Joensuu, Igor Kolotilin, Udo Conrad, Eva Stoeger, Tsafrir Mor, Heribert Warzecha, J. Chris Hall, Michael D. McLean, Eric Cox, Bert Devriendt, Andrew Potter, Ann Depicker, Vikram Virdi, Larry Holbrook, Ketan Doshi, Marike Dussault, Robert Friendship, Oksana Yarosh, Han Sang Yoo, Jacqueline MacDonald, Rima Menassa
BIOTECHNOLOGY ADVANCES
(2016)
Review
Biotechnology & Applied Microbiology
Paloma Juarez, Vikram Virdi, Ann Depicker, Diego Orzaez
PLANT BIOTECHNOLOGY JOURNAL
(2016)
Article
Virology
Francisco Miguel Cardoso, Lorena Itati Ibanez, Silvie Van den Hoecke, Sarah De Baets, Anouk Smet, Kenny Roose, Bert Schepens, Francis J. Descamps, Walter Fiers, Serge Muyldermans, Ann Depicker, Xavier Saelens
JOURNAL OF VIROLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Jorge Palaci, Vikram Virdi, Ann Depicker
PLANT BIOTECHNOLOGY JOURNAL
(2019)
Article
Biotechnology & Applied Microbiology
Vikram Virdi, Jorge Palaci, Bram Laukens, Stefan Ryckaert, Eric Cox, Erik Vanderbeke, Ann Depicker, Nico Callewaert
NATURE BIOTECHNOLOGY
(2019)
Review
Biochemical Research Methods
Henri De Greve, Vikram Virdi, Shruti Bakshi, Ann Depicker
CURRENT OPINION IN BIOTECHNOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Shruti Bakshi, Raquel Sanz Garcia, Hans Van der Weken, Ashuwini Tharad, Shubham Pandey, Paloma Juarez, Vikram Virdi, Bert Devriendt, Eric Cox, Ann Depicker
JOURNAL OF CONTROLLED RELEASE
(2020)
Article
Biochemistry & Molecular Biology
Thomas De Meyer, Elsa Arcalis, Stanislav Melnik, Katrien Maleux, Jonah Nolf, Friedrich Altmann, Ann Depicker, Eva Stoeger
PLANT MOLECULAR BIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Xue-Mei Yang, Jing-Hao Zhao, Xiao-Yu Xiong, Zhang-Wei Hu, Ji-Fen Sun, Hao Su, Yan-Jing Liu, Ling Xiang, Yong Zhu, Jin-Lu Li, Sadam Hussain Bhutto, Guo-Bang Li, Shi-Xin Zhou, Chi Li, Mei Pu, He Wang, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Wen-Ming Wang, Yan Li
Summary: The Arabidopsis RPW8.1 gene enhances disease resistance but compromises plant growth. It has been found that RPW8.1 constitutively enhances the expression of WRKY51 transcription factor and activates salicylic acid and ethylene signaling pathways. WRKY51, in turn, suppresses RPW8.1 expression, forming a feedback regulation loop.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Hannes Claeys, Eveline Neyrinck, Lies Dumoulin, Anne Pharazyn, Arne Verstichele, Laurens Pauwels, Michael L. Nuccio, Frederic Van Ex
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Melanie Ormancey, Bruno Guillotin, Camille Ribeyre, Clemence Medina, Nathanael Jariais, Helene San Clemente, Patrice Thuleau, Serge Plaza, Martina Beck, Jean-Philippe Combier
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Biying Dong, Dong Meng, Zhihua Song, Hongyan Cao, Tingting Du, Meng Qi, Shengjie Wang, Jingyi Xue, Qing Yang, Yujie Fu
Summary: This study identified the involvement of the citrate transporter CcMATE35 and the long noncoding RNA CcLTCS in aluminum stress response in pigeon pea. These findings suggest that the modules CcNFYB3-CcMATE35 and CcLTCS-CcCS jointly regulate the efflux and synthesis of citrate to enhance the resistance of pigeon pea under aluminum stress.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Review
Biotechnology & Applied Microbiology
Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Summary: Prime editing technology uses an extended guide RNA to direct a fusion peptide to a specific location in the genome, enabling precise gene editing. This groundbreaking tool has potential in improving crop varieties, but efficiency limitations exist in certain plants.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yan Xu, Jinyi Tan, Junxing Lu, Yuelin Zhang, Xin Li
Summary: Sclerotinia sclerotiorum causes white mold in economically important plants, and the discovery of SsGAP1 and SsRAS1/SsRAS2 genes as essential regulators of fungal development and virulence provides potential targets for controlling the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Weishuai Bi, Jing Liu, Yuanyuan Li, Ziwei He, Yongming Chen, Tingting Zhao, Xiangxiu Liang, Xiaodan Wang, Xiangzong Meng, Daolong Dou, Guangyuan Xu
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Shengjie You, Yu Wu, Wen Li, Xiaofeng Liu, Qinlan Tang, Fengkun Huang, Yan Li, Hsihua Wang, Mingchun Liu, Yang Zhang
Summary: In this study, a transcription factor called SlERF.G3-Like in tomato was found to play a role in regulating ethylene synthesis, cell wall degradation, and the flavonoid pathway. The researchers also discovered the interaction between SlERF.G3-Like and the master ripening regulator SlRIN, and increased the production of resveratrol derivatives through genetic manipulation. These findings provide insights into the coordination of fruit maturation and metabolic changes in tomatoes, and have implications for metabolic engineering.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Thomas R. Aalders, Mara de Sain, Fleur Gawehns, Nina Oudejans, Yoran D. Jak, Henk L. Dekker, Martijn Rep, Harrold A. van den Burg, Frank L. W. Takken
Summary: The TPL1 and TPL2 genes in tomato are closely associated with susceptibility to Fusarium wilt disease, and mutating these genes can enhance plant resistance to the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)