Article
Plant Sciences
Houssein Wehbi, Camille Soulhat, Halima Morin, Abdelhafid Bendahmane, Pierre Hilson, Oumaya Bouchabke-Coussa
Summary: Plant somatic embryogenesis (SE) is a natural process of vegetative propagation and can be induced in tissue cultures for various purposes. In this study, SE induction in monocots with Brachypodium distachyon as a model was investigated. The researchers identified the earliest stages of cell fate transition and demonstrated that secondary somatic embryos can develop within one week of in vitro culture. The fast SE induction method described in this study could contribute to the mechanistic study of SE processes and shorten the production of transgenic or gene-edited plants.
Article
Biochemistry & Molecular Biology
Safia Saada, Charles Ugochukwu Solomon, Sinead Drea
Summary: The study found that programmed cell death (PCD) occurs in developing grains of the wild model species Brachypodium distachyon. Compared to domesticated species like barley and rice, the rate and pattern of PCD in Brachypodium differ, affecting grain size and shape. This suggests that the regulation of PCD may play a role in determining grain characteristics in different plant species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jaime Barros, Him K. Shrestha, Juan C. Serrani-Yarce, Nancy L. Engle, Paul E. Abraham, Timothy J. Tschaplinski, Robert L. Hettich, Richard A. Dixon
Summary: Ammonia-lyases play a key role in coordinating the allocation of carbon for lignin synthesis and nitrogen availability for plant growth.
Article
Plant Sciences
Marlon De la Pena, Agustin Javier Marin-Pena, Leyre Urmeneta, Inmaculada Coleto, Jorge Castillo-Gonzalez, Sebastiaan M. van Liempd, Juan M. Falcon-Perez, Ana Alvarez-Fernandez, Maria Begona Gonzalez-Moro, Daniel Marino
Summary: The study found that ammonium nutrition impacts plant primary metabolism, cell wall remodelling, and redox homeostasis. Additionally, it induces proteins related to methionine metabolism and phytosiderophore synthesis. While increasing root iron content, plants fed with ammonium show higher sensitivity to iron deficiency.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Frederic Bouche, Daniel P. Woods, Julie Linden, Weiya Li, Kevin S. Mayer, Richard M. Amasino, Claire Perilleux
Summary: The proper timing of flowering in plants depends on the coordination between environmental cues and endogenous developmental programs. This paper investigates the role of the ELF3 gene in flowering regulation and shows that the elf3 mutant flowers more rapidly and is sensitive to vernalization. The study also explores the mechanism of photoperiod perception in temperate grasses, demonstrating that red light can accelerate flowering.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yongjie Meng, Kartikye Varshney, Norbert Incze, Eszter Badics, Muhammad Kamran, Sabrina F. Davies, Larissa M. F. Oppermann, Kevin Magne, Marion Dalmais, Abdel Bendahmane, Richard Sibout, John Vogel, Debbie Laudencia-Chingcuanco, Charles S. Bond, Vilmos Soos, Caroline Gutjahr, Mark T. Waters
Summary: In this study, we characterized the developmental functions of KAI2 in the grass Brachypodium distachyon. Our results showed that Bdkai2 mutants exhibited abnormal phenotypes in plant development, including increased internode elongation, reduced leaf chlorophyll levels, increased lateral roots, decreased root hair growth, and impaired root colonization by arbuscular-mycorrhizal fungi. Different hormone analogues showed distinct effects on the transcriptome via BdKAI2. Overall, our findings suggest that KAI2 plays multiple important roles in shoot development, root system development, and transcriptional regulation in grasses.
Article
Plant Sciences
Ella Ludwig, Joshua Sumner, Jeffrey Berry, Seth Polydore, Tracy Ficor, Erica Agnew, Kristina Haines, Kathleen Greenham, Noah Fahlgren, Todd C. Mockler, Malia A. Gehan
Summary: The study examines the variation in phenotypes of 149 accessions of Brachypodium distachyon under drought, heat, and combined stresses. Heat causes the most tissue damage, while the combination of stresses leads to the greatest biomass decrease. The climate of origin is significantly associated with the plant's response to stress conditions.
Article
Biochemical Research Methods
Camille Soulhat, Houssein Wehbi, Yannick Fierlej, Patrick Berquin, Thomas Girin, Pierre Hilson, Oumaya Bouchabke-Coussa
Summary: This study optimized the method for generating transgenic plants of Brachypodium distachyon (Bd), reducing the time required to produce transgenic plants to approximately 8 weeks, saving 1 to 2 months compared to previous methods, with no reduction in transformation efficiency and lower costs.
Article
Horticulture
Yali Li, Jie Xiao, Byoung Ryong Jeong
Summary: An effective indirect organogenesis system was established for Cotoneaster wilsonii Nakai.
Article
Plant Sciences
Yuxin Shen, Zhengrui Qin, Gaojie Ren, Pingchuan Deng, Wanquan Ji, Chen Jiao, Liang Wu
Summary: Alternative splicing mediated by SCL33, SCL33L, and miR156 is involved in phase transitions in Brachypodium, regulating intron assembly and miR156 targets in growth transitions.
Article
Plant Sciences
Shengbin Liu, Kevin Magne, Sylviane Daniel, Richard Sibout, Pascal Ratet
Summary: NBCL genes play important roles in the development of grasses, particularly in regulating tillering, leaf morphology, inflorescence and fruit development. Through reverse genetics and CRISPR-Cas9 technology, the functions of LAXA and CUL4 genes in the development of Brachypodium distachyon are further elucidated.
Article
Plant Sciences
Kaori Sakai, Sylvie Citerne, Sebastien Antelme, Philippe Le Bris, Sylviane Daniel, Axelle Bouder, Angelina D'Orlando, Amy Cartwright, Frederique Tellier, Stephanie Pateyron, Etienne Delannoy, Debbie Laudencia-Chingcuanco, Gregory Mouille, Jean Christophe Palauqui, John Vogel, Richard Sibout
Summary: The vascular system of plants consists of xylem and phloem tissues organized into vascular bundles. A mutation in the ERECTA gene severely affected vascular tissue organization in the model grass Brachypodium, indicating a pleiotropic role in plant development. The mutation led to defects in vasculature anastomosis, suggesting a major role of ERECTA in vascular tissue organization.
Article
Multidisciplinary Sciences
Pavel Matusinsky, Bozena Sedlakova, Dominik Blesa
Summary: This study investigated the interaction between Brachypodium distachyon and an endophytic fungus Microdochium bolleyi and its effects on the defense against the pathogen Fusarium culmorum. The results indicate that M. bolleyi can enhance the resistance of B. distachyon and wheat against F. culmorum, and certain defense-related genes are upregulated after infection.
Article
Plant Sciences
Adrianna Wiktoria Bara, Agnieszka Braszewska, Jolanta Kwasniewska
Summary: DNA methylation plays a significant role in the response of Brachypodium distachyon genome to mutagenic treatments, with alterations observed in cells post-treatment. Integrating analyses of epigenetic modifications into current plant genetic toxicology can help explain the mechanisms of DNA damage and repair in plants.
Article
Biotechnology & Applied Microbiology
Juan Carlos Serrani-Yarce, Luis Escamilla-Trevino, Jaime Barros, Lina Gallego-Giraldo, Yunqiao Pu, Art Ragauskas, Richard A. Dixon
Summary: Down-regulation of HCT1, or the co-down-regulation of both HCT genes, in B. distachyon results in less extensive changes in lignin content/composition and cell wall structure compared to dicots. However, it leads to significant improvements in biomass saccharification efficiency, making HCT a preferred target for biotechnological improvement of grasses for bioprocessing.
BIOTECHNOLOGY FOR BIOFUELS
(2021)