Article
Biotechnology & Applied Microbiology
Dennis N. Lozada, Madhav Bhatta, Danise Coon, Paul W. Bosland
Summary: Genetic diversity among New Mexican chile peppers was assessed using GBS-derived SNP markers, revealing genetic relatedness among different populations. Introducing novel alleles from other breeding programs or wild species could help increase diversity in the current germplasm. Valuable information for future association mapping and genomic selection for genetic improvement through marker-assisted breeding in New Mexican chile peppers was presented.
Article
Chemistry, Multidisciplinary
Raluca-Ioana Stefan-van Staden, Catalina Cioates Negut, Sorin Sebastian Gheorghe, Paula Sfirloaga
Summary: Two three-dimensional stochastic microsensors based on single-walled carbon nanotubes (SWCNT) and multi-walled carbon nanotubes (MWCNT) were designed to recognize and quantify IDH1 and IDH2 in biological samples. The microsensor using MWCNT demonstrated the highest sensitivity and recovery rate compared to the ELISA standard method.
Article
Biochemical Research Methods
Catalina Cioates Negut, Raluca-Ioana Stefan-van Staden, Marius Badulescu, Bogdan Bita
Summary: Disposable stochastic sensors were designed for biomedical analysis by depositing copper, graphene, and copper-graphene composite on silk textile using nanolayer deposition. Chitosan modification was applied, and the sensors were able to detect and quantify isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 in biological samples with high sensitivity and low limits of quantification. The sensors showed good correlation with enzyme-linked immunosorbent assay.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Ryan A. Herold, Raphael Reinbold, Christopher J. Schofield, Fraser A. Armstrong
Summary: Electrochemical studies reveal that nanoconfinement significantly increases the efficiency of enzyme-catalyzed cascade reactions. By using a nanoporous conducting indium tin oxide film and entrapping Isocitrate dehydrogenase 1 (IDH1), the complete electrochemical oxidation of isocitrate to 2-oxoglutarate is achieved using only the NADP(H) cofactor carried into the electrode pores. The results demonstrate the power of nanoconfinement in facilitating multistep enzyme catalysis and provide insights into the role of nicotinamide cofactors as redox carriers.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Immunology
Dongming Yan, Weicheng Li, Qibing Liu, Kun Yang
Summary: The tumor immune microenvironment and immunotherapy in IDH-mutant gliomas have been extensively studied. IDH mutations inhibit immune evasion and T-cell responses, making it a potential target for immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
T. Larsen, R. Rupp, N. C. Friggens, J. A. A. Pires
Summary: This study presents a novel method for quantifying the activity of the enzyme isocitrate dehydrogenase (IDH) in ruminant milk without pretreatment of the sample. The results show that IDH activity significantly increases during a period of nutritional restriction, accompanied by an increase in the metabolite isocitrate concentration and somatic cell count, and returns to the initial level shortly after the restriction period.
Article
Biochemistry & Molecular Biology
Ridwan Opeyemi Bello, Salihaat Toyin Okunlola, Neeraj Kumar, Omoboyede Victor, Tajudeen O. O. Jimoh, Zainab Naeem Abdulsalam, Idayat Oyinkansola Kehinde, Haruna Isiyaku Umar
Summary: A study using molecular modeling approaches identified potential dual inhibitors for the mutant IDH1/2 enzymes from the compounds of Phyllantus amarus. Ellagic acid and pinoresinol were identified as promising hits for the development of IDH1_R132H and IDH2_R140Q dual inhibitors. Further experimental studies are needed to confirm their potential as therapies.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Dong Chen, Siyuan Xia, Rukang Zhang, Yuancheng Li, Christopher A. Famulare, Hao Fan, Rong Wu, Mei Wang, Allen C. Zhu, Shannon E. Elf, Rui Su, Lei Dong, Martha Arellano, William G. Blum, Hui Mao, Sagar Lonial, Wendy Stock, Olatoyosi Odenike, Michelle Le Beau, Titus J. Boggon, Chuan He, Jianjun Chen, Xue Gao, Ross L. Levine, Jing Chen
Summary: The study revealed that mutant IDH2 in AML cells commonly has K413 acetylation, which negatively regulates its activity by attenuating dimerization and blocking substrate and cofactor binding. K413 acetylation of mitochondrial mIDH2 is achieved through a series of hierarchical phosphorylation events, optimizing the leukemogenic ability of mIDH2 in AML cells.
Review
Cell Biology
Farbod Bahreini, Elham Rayzan, Nima Rezaei
Summary: Breast cancer, a multifactorial disease, may have an increased risk due to alterations in microRNA sequences. miR-SNPs are potential biomarkers for early detection of breast cancer.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Xiefeng Yang, Chengcong Hu, Zhen Xing, Yu Lin, Yan Su, Xingfu Wang, Dairong Cao
Summary: This study aimed to evaluate the predictive value of mMRI, SWI, DWI, and DSC-PWI for Ki-67 LI and ATRX mutation status in IDH-mut astrocytoma. The results showed that these imaging modalities could accurately predict Ki-67 LI and ATRX mutation, with a possible improvement in diagnostic performance by combining mMRI and SWI.
EUROPEAN RADIOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Minjae Kim, Kai Tzu-iunn Ong, Seonah Choi, Jinyoung Yeo, Sooyon Kim, Kyunghwa Han, Ji Eun Park, Ho Sung Kim, Yoon Seong Choi, Sung Soo Ahn, Jinna Kim, Seung-Koo Lee, Beomseok Sohn
Summary: The study evaluated the performance of NLP models in predicting IDH mutation status in diffuse glioma, with BERT GCN showing the highest performance in external validation. BERT GCN was found to have superior performance compared to other NLP models and human readers in predicting IDH mutation status.
EUROPEAN RADIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ryan A. Herold, Christopher J. Schofield, Fraser A. Armstrong
Summary: The study utilizes a nanoporous electrode material, the e-Leaf, to control enzyme cascades and gain detailed kinetic insight into an anti-cancer drug mechanism. The e-Leaf allows for the quantification of IDH1 R132H inhibition kinetics and reveals factors underlying inhibitor residence time. The study highlights the importance of this method in obtaining detailed kinetic and mechanistic information that is difficult to obtain using conventional techniques.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Shuai Wang, Jingheng Wu, Wujun Zhao, Miaomiao Li, Shaoyi Li
Summary: CEBPB and P4HA2 are upregulated in IDH1 wt gliomas and associated with poor prognosis. CEBPE promotes proliferation and TMZ resistance by inducing P4HA2 expression in glioma cells.
Article
Microbiology
Dennis Winkler, Sabrina Gfrerer, Johannes Gescher
Summary: The physiology of acidophilic Micrarchaeota, such as Candidatus Micrarchaeum harzensis A_DKE, remains largely unknown due to the highly expressed hypothetical proteins. In this study, isocitrate dehydrogenase (MhIDH) from A_DKE was recombinantly produced and characterized, showing specific activity for NADP(+) with low affinity likely due to a proline residue in the NADP(+) binding pocket.
Review
Cell Biology
Xin Du, Hai Hu
Summary: 2-HG plays a significant role in 2-hydroxyglutaric aciduria, tumors with IDH1/2mt mutations, and ccRCC, being regarded as an oncometabolite. It can also accumulate in hypoxic or acidic pH conditions, and has been shown to have a vital role in the fate decision of immune cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Forestry
Florin Dinulica, Voichita Bucur, Cristian-Teofil Albu, Maria Magdalena Vasilescu, Alexandru Lucian Curtu, Norocel-Valeriu Nicolescu
Summary: The study aims to identify high-grade valuable wood resources by analyzing the acoustic quality of Norway spruce standing timber in the Romanian Carpathians. Tree phenotypes were found to be better predictors of acoustical parameters measured in the R direction compared to the L direction, with bark thickness and branch width being the most predictive factors.
EUROPEAN JOURNAL OF FOREST RESEARCH
(2021)
Article
Environmental Sciences
Ana-Maria Heres, Ion Catalin Petritan, Christof Bigler, Alexandru Lucian Curtu, Stefan Petrea, Any Mary Petritan, Josue M. Polanco-Martinez, Andreas Rigling, Jorge Curiel Yuste
Summary: Increasing worldwide droughts and heat-waves-induced tree mortality events are destabilizing forests and ecosystem services. The study found that native tree species show higher resilience to drought events, while introduced species have poor regeneration capabilities.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Multidisciplinary Sciences
Laura Cuervo-Alarcon, Matthias Arend, Markus Mueller, Christoph Sperisen, Reiner Finkeldey, Konstantin V. Krutovsky
Summary: This study identified genetic variation related to drought tolerance in saplings of European beech using a candidate gene approach, with specific genotypes showing better performance under drought treatment. The SNPs located in three candidate genes explained a significant portion of the phenotypic variance, providing valuable insight for forest conservation management strategies under future climatic conditions.
SCIENTIFIC REPORTS
(2021)
Article
Forestry
Devrim Semizer-Cuming, Igor Jerzy Chybicki, Reiner Finkeldey, Erik Dahl Kjaer
Summary: The study shows that Ash dieback reduces the reproductive success of individual ash trees, leading to an overrepresentation of healthy trees as seed and pollen parents in the next generation. Healthy trees have superior genetic dispersal and reproductive success, providing optimism for the species' future in European forests.
ANNALS OF FOREST SCIENCE
(2021)
Article
Forestry
Vladut Remus Tomsa, Alexandru Lucian Curtu, Mihai Daniel Nita
Summary: The study analyzed tree shape variability in a mixed oak forest with LiDAR technology, testing for associations between tree shape and individual heterozygosity, and comparing size differences between oak trees identified as pollen donors and non-donors. The results showed that pollen donors had significantly larger crown and trunk characteristics compared to non-donor trees.
Article
Forestry
Elena Cocorada, Alexandru Lucian Curtu, Laura Elena Nastasa, Iosif Vorovencii
Summary: Reducing student dropout rate is a major challenge for universities. This study analyzed students' dropout intention at a forestry school in Romania, finding that factors such as gender, low GPA, and low family income influence dropout intention. The results may help increase student retention.
Article
Forestry
Olympia Tsipidou, Ludger Leinemann, Georgios Korakis, Reiner Finkeldey, Oliver Gailing, Aristotelis C. Papageorgiou
Summary: This study provides insights into the genetic diversity and postglacial expansion history of beech populations in the Almopia basin, confirming it as a major refugium for the species. The results suggest the existence of two main postglacial lineages originating from this region, with genetic connections to beech populations across Europe. These southern refugial populations serve as important diversity centers that require special management and conservation efforts.
Article
Forestry
Masato Ohtani, Naoki Tani, Saneyoshi Ueno, Kentaro Uchiyama, Toshiaki Kondo, Soon Leong Lee, Kevin Kit Siong Ng, Norwati Muhammad, Reiner Finkeldey, Oliver Gailing, Mohamad Na'iem, Sapto Indrioko, Widiyatno, Iskandar Z. Siregar, Koichi Kamiya, Ko Harada, Bibian Diway, Yoshihiko Tsumura
Summary: Shorea parvifolia, an important tree species in Southeast Asia, shows different genetic diversities in different regions based on nuclear and chloroplast DNA analysis. Recent population expansion was observed in Borneo populations.
TREE GENETICS & GENOMES
(2021)
Article
Forestry
Marina Sheller, Elena Ciocirlan, Pavel Mikhaylov, Sergey Kulakov, Nadezhda Kulakova, Aleksey Ibe, Tatyana Sukhikh, Alexandru Lucian Curtu
Summary: The study on genetic diversity of Scots pine populations in Middle Siberia and the Romanian Carpathians revealed high genetic diversity, with the majority of haplotypes detected only once and a weak geographic population structure. The results suggest the importance of conservation measures for the sustainable management of Scots pine genetic resources in the study regions.
Article
Forestry
Georgeta Mihai, Alexandru-Lucian Curtu, Alin-Madalin Alexandru, Ion-Andrei Nita, Elena Ciocirlan, Marius-Victor Birsan
Summary: The most recent climate change scenarios predict a significant increase in temperature and drought frequency in Southern and Eastern Europe. This study compared the growth and drought response of Douglas fir and Norway spruce in Romania. The results showed that Douglas fir had exceptional growth capacity and higher resistance and relative resilience to drought compared to Norway spruce.
Article
Forestry
Laura Elena Nastasa, Elena Cocorada, Iosif Vorovencii, Alexandru Lucian Curtu
Summary: This article analyzes the relationship between variables in the emotional domain and academic success through two related studies. The findings show that students with higher academic performance have higher accomplishment and engagement, and exhibit weaker negative emotions towards aversive academic assessment. Female students and students with a higher ECTS load effectively utilize their emotions, have lower dropout intention, and perceive fewer barriers to completion of their studies. The academic performance at the end of the first year is influenced by factors such as GPA admission, relationships with high school teachers, expected GPA, gender, and academic resilience. Dropout intention is influenced by barriers to completion of studies, general negative emotions, negative affect related to threatened assessment situations, and managing others' emotions.
Article
Forestry
Marina Sheller, Endre Gyorgy Toth, Elena Ciocirlan, Pavel Mikhaylov, Sergey Kulakov, Nadezhda Kulakova, Natalia Melnichenko, Aleksey Ibe, Tatyana Sukhikh, Alexandru Lucian Curtu
Summary: This study assessed the genetic variation of 17 Scots pine populations in different regions of Russia. The results showed high levels of genetic diversity in all populations. Five genetic groups were identified, corresponding to different geographical areas. The European population showed the highest genetic distinctiveness. The genetic differentiation among populations only accounted for 5% of the total variance. These findings contribute to our understanding of the genetic diversity patterns of Scots pine populations in Middle Siberia and support conservation efforts of these genetic resources.
Article
Environmental Sciences
Mihnea Ioan Cezar Ciocirlan, Alexandru Lucian Curtu, Gheorghe Raul Radu
Summary: Understanding forest tree phenology is crucial for assessing the impact of environmental changes on forest ecosystems. Using remote sensing devices and machine learning techniques to observe phenology can provide fast, accurate, and unbiased results, aiding in our comprehension of ecosystem processes.
Article
Forestry
Alin-Madalin Alexandru, Georgeta Mihai, Emanuel Stoica, Alexandru Lucian Curtu
Summary: Two methods were applied to select stable and high-performing Norway spruce provenances in three provenance trials established in Romania in 1972. 81 provenances were evaluated for total and pruned height, diameter at breast height, and survival rate. Multi-trait genotype-ideotype distance index (MGIDI) and multi-trait stability index (MTSI) were used to assess performance and stability. Multiple Norway spruce provenances showed stability and high performances in all trials, providing valuable information for genetic improvement programs and seed transfer guidelines.
Article
Forestry
Maria Teodosiu, Georgeta Mihai, Elena Ciocirlan, Alexandru Lucian Curtu
Summary: In this study, genetic diversity and structure of five seed orchards of European larch were characterized using nSSR markers, and a core collection was constructed for future breeding programs. The study found that the seed orchards had slightly higher heterozygosity values compared to natural populations, but displayed heterozygote deficiency, possibly due to selective pressure. The presence of clones of autochthonous origin increased the allelic richness in the seed orchards and there were differences in clones originating from different regions.