4.7 Article

Pyrosequencing reveals how pulses influence rhizobacterial communities with feedback on wheat growth in the semiarid Prairie

Journal

PLANT AND SOIL
Volume 367, Issue 1-2, Pages 493-505

Publisher

SPRINGER
DOI: 10.1007/s11104-012-1485-z

Keywords

Pisum sativum; Triticum turgidum var durum; Wheat; Pulses; Crop rotation effect; Crop genotype; Rhizobacterial community; Pyrosequencing

Funding

  1. Novozymes
  2. Saskatchewan Pulse Growers
  3. Agriculture and Agri-Food Canada (AAFC) Matching Investment Initiative

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Evidence shows that plants modify their microbial environment leading to the crop rotation effect, but little is known about the changes in rhizobacterial community structure and functionality associated with beneficial rotation effects. Polymerase chain reaction (PCR) and 454 GS FLX amplicon pyrosequencing were used to describe the composition of the rhizobacterial community evolving under the influence of pea, a growth promoting rotation crop, and the influence of three genotypes of chickpea, a plant known as an inferior rotation crop. The growth promoting properties of these rhizobacterial communities were tested on wheat in greenhouse assays. The rhizobacterial communities selected by pea and the chickpea CDC Luna in 2008, a wet year, promoted durum wheat growth, but those selected by CDC Vanguard or CDC Frontier had no growth-promoting effect. In 2009, a dry year, the influence of plants was mitigated, indicated that moisture availability is a major driver of soil bacterial community dynamics. The effect of pulse crops on soil biological quality varies with the crop species and genotypes, and certain chickpea genotypes may induce positive rotation effects on wheat. The strength of a rotation effect on soil biological quality is modulated by the abundance of precipitation.

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