Review
Plant Sciences
Zirong Ren, Bazhen Suolang, Tadashi Fujiwara, Dan Yang, Yusuke Saijo, Toshinori Kinoshita, Yin Wang
Summary: Manipulation of plasma membrane proton-ATPase (PM H+-ATPase) has been shown to greatly improve leaf photosynthesis and plant growth. This strategy, associated with enhanced stomatal opening, holds great potential for applications in crops and nanotechnology in the future.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Katarzyna Kabala, Malgorzata Janicka
Summary: There are two ATP-dependent proton pumps in plant cells, the Plasma membrane H+-ATPase (PM H+-ATPase) and the vacuolar H+-ATPase (V-ATPase). While the PM H+-ATPase transfers protons from the cytoplasm to the apoplast, the V-ATPase pumps protons into the organelle lumen. These enzymes belong to different protein families and have distinct structures and mechanisms of action. However, they can be regulated by the same mechanisms and may coordinate in certain processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Le Liu, M. Arif Ashraf, Taylor Morrow, Michelle Facette
Summary: Stomata in grasses have fast movements due to their unique guard cell and subsidiary cell structures. Subsidiary cells are essential for stomatal function and their defects can lead to improper closure. Maize PAN2 gene, orthologous to Arabidopsis GHR1 gene, acts downstream of Ca2+ to promote stomatal closure, suggesting potential pathway differences.
Article
Plant Sciences
Hui-Qian Cheng, Ying-Ning Zou, Qiang-Sheng Wu, Kamil Kuca
Summary: The research found that arbuscular mycorrhizal fungi can enhance plant resistance to drought. AMF can stimulate H+-ATPase activity and PtAHA2 gene expression, promoting nutrient uptake and root growth in plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Plant Sciences
Thorsten Seidel
Summary: V-ATPase is the dominant proton pump in plant cells, playing crucial roles in cytosolic pH homeostasis, endomembrane transport processes, and stress responses. Its complex structure and multiple subunit isoforms provide structural flexibility, but also require sophisticated assembly machinery and transport routes in cells.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Takuto Fujii, Kenji Sugimoto, Takafumi Noda, Takahiro Shimizu, Yuji Matsuya, Hideki Sakai
Summary: A newly synthesized dihydropyrazole derivative KYY-008 was found to selectively inhibit the activity of gastric H+,K+-ATPase, with no effect on other P2-type ATPases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Ryuta Kanai, Flemming Cornelius, Bente Vilsen, Chikashi Toyoshima
Summary: Cryo-electron microscopy was used to study the structure of Na+, K+-ATPase and its variations. The results showed that different reaction states exhibited different biochemical characteristics, and the presence of Mg2+ ions in the transmembrane cation binding sites played a crucial role in the reactions. The binding mechanism of the new-generation cardiotonic steroids (CTSs) did not significantly affect the structure of NKA, and the phospholipid molecules were better resolved in the electron microscopy maps compared to X-ray structures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Plant Sciences
Satoru N. Kinoshita, Toshinori Kinoshita
Summary: Cytosolic and extracellular pH homeostasis is vital for various physiological processes in plants. The plasma membrane H+-ATPase plays a critical role in maintaining pH homeostasis and its activity is regulated by protein abundance and post-translational modifications. Phosphorylation of the penultimate threonine of the carboxy terminus can activate the plasma membrane H+-ATPase. This review summarizes recent findings on the regulation of plasma membrane H+-ATPase in photosynthetic tissues of plants, with a focus on its mechanisms and physiological roles.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Michelle E. Maxson, Yazan M. Abbas, Jing Ze Wu, Jonathan D. Plumb, Sergio Grinstein, John L. Rubinstein
Summary: This study shows that proton-pumping vacuolar H+ ATPases (V-ATPases) are responsible for the acidic environment in secretory and endocytic organelles. The researchers developed a new probe derived from SidK protein to visualize and quantify V-ATPases with high specificity. They found that the density of V-ATPases increases during phagosome maturation and their distribution varies in lysosomes depending on their subcellular localization.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Margaret R. Young, Sabine Heit, Maike Bublitz
Summary: This article reviews the current progress in the study of the structure, function, and biogenesis of fungal Pma1 and introduces the recently determined high-resolution structures of Pma1 using cryo-electron microscopy, providing new insights into its stability, regulated activity, and druggability.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Agriculture, Multidisciplinary
Jun-xing SONG, Ying-can CHEN, Zhao-hui LU, Guang-ping ZHAO, Xiao-li WANG, Rui ZHAI, Zhi-gang WANG, Cheng-quan YANG, Ling-fei XU
Summary: This study identified the PbPH5 gene as a key regulator of malic acid accumulation in pears. PbPH5 overexpression significantly increased malic acid content in pear fruit, while silencing PbPH5 decreased malic acid levels. These findings suggest that PbPH5 plays a conserved role in regulating malic acid accumulation in pears.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Plant Sciences
Yael Grunwald, Noa Wigoda, Nir Sade, Adi Yaaran, Tanmayee Torne, Sanbon Chaka Gosa, Nava Moran, Menachem Moshelion
Summary: The study identified the crucial role of AHA2 protein in regulating xylem sap pH and leaf radial water fluxes within the leaf vascular bundle sheath cells. Manipulation of AHA2 activity significantly affected the acid-base balance of xylem sap and leaf hydraulic conductance, demonstrating a causal relationship between AHA2 activity and leaf radial hydraulic water conductance.
Article
Biochemistry & Molecular Biology
Magdalena Zboinska, Luis C. Romero, Cecilia Gotor, Katarzyna Kabala
Summary: This study aimed to investigate the role of jasmonic acid (JA) in the regulation of V-ATPase in cucumber roots and its relationship with other regulators. The researchers found that JA acts as a repressor of V-ATPase, leading to a decrease in the protein amount of certain subunits, induction of oxidative stress, and downregulation of a specific posttranslational modification. They also suggest that H2S and H2O2 might be downstream components of JA-dependent negative regulation of the proton pump. Furthermore, they compared the signaling pathways induced by JA and cadmium, and found that multiple pathways are involved in the downregulation of V-ATPase in cucumber roots.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kevin Su, Michael P. Collins, Christina M. McGuire, Mohammed A. Alshagawi, Mariam K. Alamoudi, Zhen Li, Michael Forgac
Summary: The a4 isoform of the V-ATPase plays a significant role in the migration and invasion of breast cancer cells, as well as in the growth of tumors in vivo. Targeting this isoform may represent a novel therapeutic strategy to limit breast cancer growth and metastasis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Toxicology
Xinling Zhang, Yue Wang, Wenting Luo, Marie M. Zhu, Changjiang Huang
Summary: The study found that PPIs antagonized the effect of RC48ADC, potentially through inhibition of vacuolar H+-ATPase activity, while cimetidine did not have this effect. Therefore, the combination of cimetidine with ADCs could be a choice to prevent adverse drug interactions.
TOXICOLOGY IN VITRO
(2022)