Journal
PLANT AND CELL PHYSIOLOGY
Volume 52, Issue 2, Pages 448-467Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcr002
Keywords
AtPol lambda; DNA damage; DNA polymerase; Nucleotide excision repair; UV-B
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Funding
- Department of Science and Technology (DST) Science and Engineering Research Council [SR/FT/LS-070/2007]
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Plants are constantly exposed to a wide range of environmental genotoxic stress factors including obligatory exposure to UV radiation in sunlight. Here, we report the functional characterization of a DNA repair protein, AtPol lambda, a homolog of mammalian DNA polymerase lambda in Arabidopsis, in relation to its role in repair of UV-B-induced DNA damage during early stages of seedling development. The abundance of the AtPol lambda transcript and the protein levels were distinctly increased in response to UV-B irradiation in 6-day-old wild-type seedlings. Growth of atpol lambda mutant seedlings, deficient in AtPol lambda expression, was more sensitive to UV-B radiation compared with wild-type plants when seeds were exposed to UV-B radiation before germination. The atpol lambda mutants showed accumulation of relatively higher amounts of DNA lesions than wild-type plants following UV-B exposure and were less proficient in repair of UV-induced DNA damage. Increased accumulation of AtPol lambda protein in UV-B-irradiated 6-day-old wild-type seedlings during the dark recovery period has indicated a possible role for the protein in repair of UV-B-induced lesions in the dark. Overexpression of AtPol lambda in the atpol lambda mutant line partially complemented the repair proficiency of UV-B-induced DNA damage. In vitro repair synthesis assays using whole-cell extracts from the wild-type and atpol lambda mutant line have further demonstrated the role of AtPol lambda in repair synthesis of UV-B-damaged DNA in the dark through an excision repair mechanism. Overall, our results have indicated the possible involvement of AtPol lambda in a plant's response for repair of UV-B-mediated DNA damage during seedling development.
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