Article
Biochemistry & Molecular Biology
Viktor A. Anashkin, Alexander A. Baykov
Summary: The study revealed that membrane-integral inorganic pyrophosphatases exhibit structural symmetry, but asymmetry increased upon substrate binding, opening water channel exit. This asymmetrical change may have a regulatory function in the vacuole.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Amarachukwu Faith Onuh, Kyoko Miwa
Summary: The aim of this study was to explore the physiological role of AVP2;1 in Arabidopsis thaliana. The mutant carrying a missense mutation in AVP2;1 showed increased primary root growth under low boron conditions but no significant difference under normal boron condition compared to wild type plants. Root cell observation revealed changes in the length and number of cells, as well as the concentration of calcium and specific sugars in the mutant compared to wild type plants under low boron.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Hikari Matsumoto, Yusuke Kimata, Takumi Higaki, Tetsuya Higashiyama, Minako Ueda
Summary: This study reveals that in Arabidopsis zygotes, vacuolar contents are exchanged dynamically between the apical and basal ends via a tubular network, with vacuoles migrating towards the basal end. Mutants lacking key vacuolar membrane proteins failed to maintain asymmetric vacuolar distribution, leading to symmetric cell division. The study also highlights the importance of YDA-mediated directional migration for establishing polar vacuolar distribution in zygotes.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Plant Sciences
Huan Long, Jinhua Fang, Lian Ye, Baolong Zhang, Colleen Hui, Xuan Deng, Sabeeha S. Merchant, Kaiyao Huang
Summary: This study optimized a method for purifying lysosome-related organelles (LROs) from two different populations of cells and discovered differences in morphology and function between them. It found that LROs in stationary phase cells had non-membrane-bound polyphosphate granules for phosphorus storage, while those in iron-overloaded cells were similar to acidocalcisomes with membrane boundaries and larger polyphosphate granules. Additionally, the study established a method for quantifying the sequestration capacity of LROs for trace metals and identified 113 putative acidocalcisome proteins through comparative proteomic analysis.
Article
Plant Sciences
Hiroyuki Ishida, Yu Okashita, Hiromi Ishida, Makoto Hayashi, Masanori Izumi, Amane Makino, Nazmul H. Bhuiyan, Klaas J. van Wijk
Summary: Chloroplasts, and plastids in general, are major sources of carbon and nitrogen recycling, being partially degraded by piecemeal autophagy. A mutant, gfs9-5, with defective membrane-trafficking factor GFS9, accumulates plastid bodies (PBs) and shows increased autophagic flux, suggesting a potential connection between GFS9 and autophagy in the process of piecemeal plastid autophagy.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Orion Shih, Yi-Qi Yeh, Kuei-Fen Liao, Kun-Mou Li, Jia-Yin Tsai, Chieh-Chin Li, Yun-Wei Chiang, Richard K. Heenan, Yuh-Ju Sun, U-Ser Jeng
Summary: This study reveals the conformations and compositions of a proton-translocating pyrophosphatase Vigna radiata H+-PPase (VrPPase) in either detergent molecules or lipid nanodisc using SEC-SAXS, providing a deeper understanding of membrane-protein complexes.
MATERIALS CHEMISTRY AND PHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Guihong Liang, Haixing Song, Yan Xiao, Zhenhua Zhang
Summary: This study explored the impact of V-ATPase activity in plant vacuoles on growth and development. The lack of two tonoplast-localized subunits of V-ATPase resulted in defective growth and excessive ammonium accumulation in plants, which could be alleviated by potassium supplementation. This research provided new insights into the relationship between V-ATPase, growth, and ammonium accumulation, while highlighting the role of potassium in mitigating ammonium toxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Yuta Otsuka, Hirokazu Tsukaya
Summary: Organisms exhibit various three-dimensional structures that change over time, including twisting which is linked to essential adaptive functions. Despite its significance, there is a lack of rigorous methods for quantifying twisting in plant organs. This study introduces a novel approach for quantifying the 3D twisting angles of Arabidopsis petioles and highlights the potential impact of differential growth on pronounced twisting.
JOURNAL OF PLANT RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Anssi M. Malinen, Viktor A. Anashkin, Victor N. Orlov, Alexander Bogachev, Reijo Lahti, Alexander A. Baykov
Summary: This study provides functional evidence supporting the coupling mechanism of H+-pumping and Na+-pumping by membrane-bound pyrophosphatase (mPPase) found in bacteria and archaea. The research also shows that pyrophosphate hydrolysis is the rate-limiting step in mPPase catalysis and the hydrolysis-generated proton plays a crucial role in the transport process.
Review
Plant Sciences
Thorsten Seidel
Summary: V-ATPase is the dominant proton pump in plant cells, playing crucial roles in cytosolic pH homeostasis, endomembrane transport processes, and stress responses. Its complex structure and multiple subunit isoforms provide structural flexibility, but also require sophisticated assembly machinery and transport routes in cells.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Wenxiu Ye, Shota Koya, Yuki Hayashi, Huimin Jiang, Takaya Oishi, Kyohei Kato, Kohei Fukatsu, Toshinori Kinoshita
Summary: Stomatal guard cells are specialized cells that respond to stimuli such as blue light and abscisic acid for regulating stomatal aperture. Several GC-specific genes, including ALMT6, were identified to play important roles in stomatal movement, particularly in the opening process. The study highlights the significance of GC-specific genes in stomatal regulation and provides new insights into the molecular mechanisms involved.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Developmental Biology
Timon W. Matz, Yang Wang, Ritika Kulshreshtha, Arun Sampathkumar, Zoran Nikoloski
Summary: In this study, the authors used a network representation of live cell imaging data from shoot apical meristems (SAMs) in Arabidopsis thaliana to predict cell division events and their consequences at the tissue level. They found that a support vector machine classifier based on the SAM network properties is able to predict cell division events with high accuracy. Furthermore, the combination of topological and biological properties can improve the prediction accuracy of resulting changes in tissue topology triggered by cell division. The study also highlights the importance of microtubule-mediated cell-to-cell growth coordination in influencing tissue-level topology.
Article
Plant Sciences
Ewa Mlodzinska-Michta
Summary: Phosphate deficiency is a major abiotic stress that negatively affects crop growth and productivity. This study focuses on the expression and function of plasma membrane H+-ATPase genes in Arabidopsis thaliana roots, and suggests their involvement in root growth regulation and response to phosphate supply.
ACTA PHYSIOLOGIAE PLANTARUM
(2022)
Article
Biochemistry & Molecular Biology
Sherif A. Abdel-Gawad, Hany H. Arab, Alhumaidi B. Alabbas
Summary: Monitoring and quantification of active pharmaceutical ingredients in the environment, especially proton pump inhibitors, are crucial for human health. Ultra-performance liquid chromatography has been successfully used to separate and quantify these drugs in pharmaceutical industrial wastewater samples.
Article
Plant Sciences
Hilary Hunt, Nico Brueggen, Alexander Galle, Sandy Vanderauwera, Claus Frohberg, Alisdair R. Fernie, Uwe Sonnewald, Lee J. Sweetlove
Summary: Companion cells and sieve elements in vascular plants have important yet unknown metabolic functions. Using a tissue-scale flux balance analysis (FBA) model, we investigate the metabolism of phloem loading in Arabidopsis leaves. Our model suggests a different role for companion cell chloroplasts compared to mesophyll chloroplasts, indicating that companion cell chloroplasts mainly provide ATP to the cytosol rather than capturing carbon. We also found that certain amino acids synthesized in the phloem tissue lead to more efficient phloem loading.
Article
Plant Sciences
Tomoko Hirano, Teun Munnik, Masa H. Sato
PLANT AND CELL PHYSIOLOGY
(2017)
Article
Plant Sciences
Tomoko Hirano, Kelly Stecker, Teun Munnik, Haoxing Xu, Masa H. Sato
PLANT AND CELL PHYSIOLOGY
(2017)
Article
Cell Biology
Misoon Park, Cornelia Krause, Matthias Karnahl, Ilka Reichardt, Farid El Kasmi, Ulrike Mayer, York-Dieter Stierhof, Ulrike Hiller, Georg Strompen, Martin Bayer, Marika Kientz, Masa H. Sato, Marc T. Nishimura, Jeffery L. Dangl, Anton A. Sanderfoot, Gerd Juergens
DEVELOPMENTAL CELL
(2018)
Review
Plant Sciences
Shoji Segami, Mariko Asaoka, Satoru Kinoshita, Mayu Fukuda, Yoichi Nakanishi, Masayoshi Maeshima
PLANT AND CELL PHYSIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Shoji Segami, Takaaki Tomoyama, Shingo Sakamoto, Shizuka Gunji, Mayu Fukuda, Satoru Kinoshita, Nobutaka Mitsuda, Ali Ferjani, Masayoshi Maeshima
Article
Biochemistry & Molecular Biology
Young Koung Lee, Ji Ye Rhee, Seong Hee Lee, Gap Chae Chung, Soon Ju Park, Shoji Segami, Masayohi Maeshima, Giltsu Choi
PLANT MOLECULAR BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Ali Ferjani, Kensuke Kawade, Mariko Asaoka, Akira Oikawa, Takashi Okada, Atsushi Mochizuki, Masayoshi Maeshima, Masami Yokota Hirai, Kazuki Saito, Hirokazu Tsukaya
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Yusuke Kimata, Takehide Kato, Takumi Higaki, Daisuke Kurihara, Tomomi Yamada, Shoji Segami, Miyo Terao Morita, Masayoshi Maeshima, Seiichiro Hasezawa, Tetsuya Higashiyama, Masao Tasaka, Minako Ueda
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Correction
Multidisciplinary Sciences
Ali Ferjani, Kensuke Kawade, Mariko Asaoka, Akira Oikawa, Takashi Okada, Atsushi Mochizuki, Masayoshi Maeshima, Masami Yokota Hirai, Kazuki Saito, Hirokazu Tsukaya
SCIENTIFIC REPORTS
(2019)
Article
Plant Sciences
Mayu Fukuda, Marika Mieda, Ryosuke Sato, Satoru Kinoshita, Takaaki Tomoyama, Ali Ferjani, Masayoshi Maeshima, Shoji Segami
FRONTIERS IN PLANT SCIENCE
(2020)
Letter
Plant Sciences
Yoichi Nakanishi, Terumi Kawashima, Mayuko Naganawa, Toshiyuki Mikami, Masayoshi Maeshima, Sumie Ishiguro
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuki Sakamoto, Ayako Kawamura, Takamasa Suzuki, Shoji Segami, Masayoshi Maeshima, Stefanie Polyn, Lieven De Veylder, Keiko Sugimoto
Summary: This study demonstrates the importance of transcriptional activation of auxin biosynthesis in reprogramming differentiated plant cells. Histone acetylation facilitates the activation of PLETHORAs, leading to the induction of downstream YUCCA1 gene. Auxin biosynthesis is required for initial cell division and the expression of MYB3R4 gene.
Article
Biochemistry & Molecular Biology
Misaki Koguchi, Kanako Yamasaki, Tomoko Hirano, Masa H. Sato
PLANT SIGNALING & BEHAVIOR
(2017)