Journal
PLACENTA
Volume 35, Issue 8, Pages 658-660Publisher
W B SAUNDERS CO LTD
DOI: 10.1016/j.placenta.2014.06.003
Keywords
OAT4; Estrogen synthesis; Protein kinase A; Dehydroepiandrosterone sulfate; Syncytiotrophoblast
Funding
- JSPS KAKENHI [21890246, 23790199, 25560063, 25860396]
- MEXT-Supported Program for Strategic Research at Private Universities
- Food Safety Commission of Japan (Cabinet Office) [1107]
- Takeda Science Foundation
- Nakatomi Foundation
- Grants-in-Aid for Scientific Research [25860396, 21890246, 23790199, 25560063] Funding Source: KAKEN
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The purpose of this study is to assess the role of the protein kinase A (PKA) in regulating uptake of dehydroepiandrosterone sulfate (DHEAS), an estrogen precursor, by syncytiotrophoblasts. Forskolin, a PKA activator, significantly increased [H-3]DHEAS uptake and the mRNA expression levels of organic anion transporter (OAT) 4 and CYP19A1 in choriocarcinoma JEG-3 cells, while other steroid sulfate transporters present in the placenta showed no change in expression level. KT5720, a PKA inhibitor, attenuated these effects of forskolin. Accordingly, the PKA pathway appears to play an important role in estrogen synthesis by cooperatively regulating OAT4 and steroidogenic enzymes in syncytiotrophoblasts. (C) 2014 Elsevier Ltd. All rights reserved.
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