Journal
PLACENTA
Volume 35, Issue 7, Pages 483-490Publisher
W B SAUNDERS CO LTD
DOI: 10.1016/j.placenta.2014.04.001
Keywords
Preeclampsia; Placental mesenchymal stromal cells; Placenta; Activating protein 1; junB; Cyclin D1
Funding
- Regione Piemonte within the program for Ricerca Sanitaria Finalizzata [204 30042009]
- Fondazione Cariplo [2011-0495]
- University of Turin
- Regione Piemonte
- Compagnia di San Paolo
- Fondazione Cariplo
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Introduction: In the present study, we characterized the expression of Activating Protein 1 (AP-1) factors, key cell cycle regulators, in primary placental mesenchymal stromal cells (PDMSCs) derived from normal and preeclamptic (PE) pregnancies with fetal-placental compromise. Methods: PDMSCs were isolated from control (n = 20) and preeclamptic (n = 24) placentae. AP-1 expression was determined by semi-quantitative RT-PCR (sqRT-PCR), Real Time PCR and Western Blot assay. PDMSCs were plated and JunB siRNA was performed. JunB and Cyclin-D1 expression were assessed by Real Time and Western Blot analyses. Results: JunB expression was significantly increased while Cyclin-D1 expression was significantly down-regulated in PE relative to control PDMSCs. JunB siRNA was accompanied by JunB down-regulation and increased Cyclin-D1 in normal PDMSCs. Conclusions: We described, for the first time, AP-1 expression in PDMSCs derived from physiological and PE placentae. Importantly, we demonstrated that JunB over-expression in PE-PDMSCs affects Cyclin-D1 regulation. Our data suggest a possible contribution of these pathological placental cells to the altered cell cycle regulation typical of preeclamptic placentae. (C) 2014 Elsevier Ltd. All rights reserved.
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