Journal
PLACENTA
Volume 35, Issue 3, Pages 223-227Publisher
W B SAUNDERS CO LTD
DOI: 10.1016/j.placenta.2014.01.005
Keywords
Immunoelectron; Electron microscopy; Correlative microscopy; Placenta; Syncytiotrophoblast; Antiphospholipid antibody
Funding
- University of Auckland
- Auckland Medical Research Foundation
- School of Medicine, University of Auckland
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Immunoelectron microscopy is wrought with technical limitations that complicate its use. However, advances in correlative light and electron microscopy have recently lead to improvements in this field. We report the development of a semi-correlative approach to investigate the ultrastructural location of an antiphospholipid antibody within the syncytiotrophoblast This method offers several advantages over existing methodologies, since it preserves antigenicity, shows good immunolabel penetrability and does not require specialized equipment. The use of a pre-embedding screen has also allowed us to target individual placental villi and overcome sampling limitations of the electron microscope. This simple, cost-effective method is likely to find widespread application in placental research. (C) 2014 Elsevier Ltd. All rights reserved.
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