Review
Cell Biology
Phelipe O. Favaron, Anthony M. Carter
Summary: Giant cells, known as trophoblast giant cells (TGCs), play a significant role in placentation in cricetid rodents. They exhibit a high degree of polyploidy and can be found at fixed locations in the placenta or migrate deep into the uterus. The distribution of TGCs in cricetids is compared to that of murid rodents, revealing similarities in certain locations and characteristics of TGCs. The polyploidy of TGCs in cricetids amplifies selective genomic regions, suggesting the need for further study and research on these cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Immunology
Xiao-Wei Wei, Yu-Chen Zhang, Fan Wu, Fu-Ju Tian, Yi Lin
Summary: Uterine spiral artery remodeling plays a crucial role in successful pregnancy, which is regulated by uterine NK cells and extravillous trophoblasts.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Endocrinology & Metabolism
Yueyue Zhu, Xiaorui Liu, Yichi Xu, Yi Lin
Summary: Uterine spiral artery remodeling is crucial for fetal growth and pregnancy outcomes. Hyperglycemia disrupts trophoblast proliferation and invasion by inhibiting epithelial-mesenchymal transition and altering the expression of related proteases and angiogenic factors. It also affects cellular crosstalk between immune cells, trophoblasts, and vascular cells, leading to failed spiral artery remodeling. This review provides insights into the molecular mechanisms and signaling pathways of hyperglycemia on trophoblast functions and uterine spiral artery remodeling.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Vinay Shukla, Michael J. Soares
Summary: This article introduces the characteristics of hemochorial placentation and trophoblast-guided uterine spiral artery remodeling in rats, which are similar to human placental development. Inadequacies in these processes negatively affect fetal growth and development. The application of genome editing technology and phenotyping strategies provides new insights into the biological characteristics of rat hemochorial placentation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Jiaye Chen, Tinglin Song, Sizhu Yang, Qingxue Meng, Xiaoyu Han, Ze Wu, Jung-Chien Cheng, Lanlan Fang
Summary: This study aimed to investigate the effect of GDF-8 on invasion and MMP2 expression in human EVT cells, and found that Snail is a key factor in GDF-8-induced MMP2 expression and cell invasion.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Heyam Hayder, Guodong Fu, Lubna Nadeem, Jacob A. O'Brien, Stephen J. Lye, Chun Peng
Summary: The overexpression of miR-210-3p reduces trophoblast migration and invasion, affects EVT outgrowth in early pregnancy explants, and decreases the ability of trophoblast to form endothelial-like networks. Furthermore, miR-210-3p downregulates the expression of certain cytokines, potentially influencing EVT invasion and immune cell recruitment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Mair Zamir, D. Michael Nelson, Yehuda Ginosar
Summary: During pregnancy, a major increase in uteroplacental blood flow is achieved through the geometric transformation of uterine spiral arteries and trophoblast invasion, which significantly reduces resistance to flow, critical for normal pregnancy. Our research identifies key variables in this hemodynamic change and establishes a numerical threshold for adequate trophoblast invasion, potentially serving as a diagnostic marker for high-risk pregnancies in the future.
JOURNAL OF APPLIED PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
Yong Pu, Jeremy Gingrich, Almudena Veiga-Lopez
Summary: Placental trophoblast cell invasion into the maternal uterus is a crucial and complex event in the formation of the maternal-fetal interface. A 3D PDMS microfluidic platform has been developed to accurately evaluate trophoblast invasion, allowing for real-time monitoring, imaging, and collection of invasive cells. This platform provides insights into pathways regulating trophoblast invasion and is useful for toxicological screening.
Article
Cell Biology
Asli Ozmen, Ozlem Guzeloglu-Kayisli, Selcuk Tabak, Xiaofang Guo, Nihan Semerci, Chinedu Nwabuobi, Kellie Larsen, Ali Wells, Asli Uyar, Sefa Arlier, Ishani Wickramage, Hasan Alhasan, Hana Totary-Jain, Frederick Schatz, Anthony O. Odibo, Charles J. Lockwood, Umit A. Kayisli
Summary: In preeclampsia, the competition binding of IL-6 and IL-11 to gp130 impairs the function and morphology of extravillous trophoblast cells (EVT), and the reduced expression of ISG15 is associated with this effect.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Kaela M. Varberg, Michael J. Soares
Summary: Uterine spiral artery remodeling during placentation is crucial for maternal-fetal blood flow, with trophoblast cell invasion playing a key role in this process. Deficient trophoblast invasion can lead to pregnancy disorders.
Article
Cell Biology
Danai Georgiadou, Souad Boussata, Remco Keijser, Dianta A. M. Janssen, Gijs B. Afink, Marie van Dijk
Summary: Mutations in the LINC-HELLP non-coding RNA (HELLPAR) are associated with familial forms of the pregnancy-specific HELLP syndrome, impacting EVT differentiation and the binding of RNA splicing complex proteins PCBP1, PCBP2, and YBX1 to LINC-HELLP. Knockdown of PCBP2, and to a lesser extent YBX1 and PCBP1, leads to failure of EVTs to differentiate towards an invasive phenotype. PCBP2 silencing alters splicing for genes related to cellular assembly, organization, function, maintenance, growth, proliferation, and the cell cycle, indicating its crucial role in EVT differentiation mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Tae Yokouchi-Konishi, Yongjie Liu, Liping Feng
Summary: Proper extravillous trophoblast invasion is important for normal placenta development and pregnancy. This study reveals that attenuation of progesterone receptor membrane component 2 promotes cell proliferation, invasion, and angiogenesis in extravillous trophoblasts by activating hypoxia-inducible factor 1 alpha signaling. This discovery provides new insights into the mechanisms of trophoblast invasion.
BIOLOGY OF REPRODUCTION
(2023)
Article
Pharmacology & Pharmacy
Dan Qi, Jingyuan Lu, Ziyi Fu, Shanshan Lv, Lili Hou
Summary: In this study, psoralen was found to enhance the proliferation, migration, and invasion abilities of trophoblast cells by upregulating the expression and activity of MMP-2 and MMP-9, and promoting the nuclear translocation of NF-κB p65. Psoralen may serve as a potential repurpose drug candidate for inducing trophoblast cell migration and invasion through the NF-κB pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Tanvi Tavarna, Bryce Wolfe, Xiao-jun Wu, Leticia Reyes
Summary: This study found that impaired spiral artery remodeling caused by Porphyromonas gingivalis infection was associated with disturbances in uterine natural killer cells and placental bed decidual stroma.
SCIENTIFIC REPORTS
(2022)
Article
Endocrinology & Metabolism
Yingqi Nong, Shifen Li, Wenjuan Liu, Xiqian Zhang, Lin Fan, Ye Chen, Qianwen Huang, Qianyu Zhang, Fenghua Liu
Summary: The study found that AQP3 significantly promotes the migration and invasion capabilities of human extravillous trophoblasts. This effect may be mediated by regulating genes related to adhesion and signaling pathways, such as PDGF-B, PIK3/AKT, NF-kappa B, and TNF. Further verification is required to confirm this mechanism.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Medicine, Research & Experimental
Abbie Dodd, Az Alddien Natfji, Angelos Evangelinos, Antonella Grigoletto, Gianfranco Pasut, Frances Beards, Lewis Renshall, Helen M. Osborn, Francesca Greco, Lynda K. Harris
Summary: This study evaluated the feasibility of non-prodrug PEG-drug conjugates in reducing drug accumulation in placental tissues. Results showed that PEG was biocompatible with human placenta, with no impact on cell proliferation or apoptosis rates. Treatment with PEG did not cause significant changes in lactate dehydrogenase levels or human chorionic gonadotropin release. Cellular uptake studies demonstrated that conjugating Cy5.5 and haloperidol to PEG resulted in a significant reduction (up to approximately 40-fold) in their uptake by the placenta.
MOLECULAR PHARMACEUTICS
(2022)
Article
Obstetrics & Gynecology
Sourima Biswas Shivhare, Qinsheng Lu, Dingqian Sun, Huomei Hou, Judith N. Bulmer, Barbara A. Innes, Dharani K. Hapangama, Gendie E. Lash
Summary: This study investigated the expression pattern of PDGF-BB and its receptors in the endometrium of healthy control women and those with AUB-E across the menstrual cycle. The results showed a reduction in endothelial cell expression of PDGF-BB and PDGFR alpha in the mid and late secretory phases in AUB-E, which may contribute to the reduced vascular maturation observed in these women.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Reproductive Biology
Zhiyong Zou, Lynda K. Harris, Karen Forbes, Alexander E. P. Heazell
Summary: Fetal growth restriction (FGR) is closely related to placental dysfunction and hypoxia. The decreased signaling of estrogen-related receptor gamma (ESRRG) in hypoxic conditions may contribute to hypoxia-induced placental dysfunction in FGR. The ESRRG agonist DY131 has the potential to restore hypoxic damages and could be used as a therapeutic approach for the treatment of placental dysfunction.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
Zhiyong Zou, Lynda K. Harris, Karen Forbes, Alexander E. P. Heazell
Summary: This study demonstrates that BPA affects the ESRRG signaling pathway in a sex-specific manner in human placentas, providing a possible biological mechanism to explain the differential effects of BPA exposure on male and female fetuses observed in epidemiological studies.
BIOLOGY OF REPRODUCTION
(2022)
Article
Immunology
Yue Pan, Li Yang, Danyang Chen, Huomei Hou, Min Zhang, Miaojuan Chen, Fen Ning, Qinsheng Lu, Minguan Zhao, Li Li, Gendie E. Lash
Summary: Remodeling of the uterine spiral arteries is crucial for successful pregnancy. Decidual macrophages play a significant role in this process by secreting MMP3, which facilitates breakdown of the extracellular matrix during vascular remodeling.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2022)
Article
Developmental Biology
Hanbo Liu, Fen Ning, Gendie E. Lash
Summary: Uterine spiral artery remodeling is a crucial adaptation of pregnancy, and its failure is associated with obstetric complications. While the initial stages of remodeling have been studied, there is limited knowledge on the fate of vascular smooth muscle cells (VSMCs).
Article
Developmental Biology
YuJia Wang, Yingye Zhang, Yongqi Wu, Yunbiao He, Jingwen Xiang, Jieqiong Huang, Gendie E. Lash, Ping Li
Summary: The downregulation of SIRT1 and the upregulation of senescence- and ECM-related proteins are observed in premature aging placenta with preeclampsia. Activation of SIRT1 can abolish senescence in syncytialized BeWo cells, while knockdown of SIRT1 restores these effects.
Article
Pharmacology & Pharmacy
Azziza Zaabalawi, Lewis Renshall, Frances Beards, Adam P. Lightfoot, Hans Degens, Yvonne Alexander, Ragheb Hasan, Haris Bilal, Brigitte A. Graf, Lynda K. Harris, May Azzawi
Summary: This study investigates the use of liposome-encapsulated TMS to improve vasodilator function in patients undergoing CABG. The results demonstrate that TMS-loaded liposomes can enhance vasodilation by inhibiting CYP1B1 and reducing reactive oxygen species, and suggest the potential of TMS-loaded liposomes as a therapeutic strategy for the treatment of hypertension in CABG patients.
Article
Oncology
Miaojuan Chen, Yue Pan, Hanbo Liu, Fen Ning, Qinsheng Lu, Yaoyun Duan, Xiaowen Gan, Shenjiao Lu, Huomei Hou, Min Zhang, Yun Tian, Gendie E. Lash
Summary: In this study, a novel role of ezrin in breast cancer liver metastasis (BCLM) was explored. It was found that ezrin promotes cell proliferation, migration, and invasion through activation of the Notch1 signaling pathway via furin-like convertase. These findings provide insights into the mechanism of ezrin in breast cancer progression and offer a potential target for the treatment of BCLM.
Article
Developmental Biology
Lindong Zhang, Jing Liu, Xiaoqian Feng, Gendie E. Lash
Summary: Spiral artery remodeling is a crucial process for providing maternal blood to the placenta. Failure of this process is associated with various obstetric complications. While the morphological features of spiral artery remodeling have been described, recent research has focused on understanding the cellular and molecular triggers involved. This review discusses the current knowledge of spiral artery remodeling, particularly the loss of vascular smooth muscle cells, and examines which stage defects could lead to pathological pregnancies.
Article
Oncology
Paraskevi Mallini, Miaojuan Chen, Kamilla Mahkamova, Thomas W. J. Lennard, Yue Pan, Dan Wei, Katherine Stemke-Hale, John A. Kirby, Gendie E. Lash, Annette Meeson
Summary: Epithelial-to-mesenchymal transition (EMT) plays a vital role in regulating cancer cell behavior, allowing them to acquire stem cell characteristics and become more migratory and invasive. EMT can be driven by various mechanisms, such as the TGF-beta 1 signaling pathway and/or hypoxia. This study investigated the effects of CoCl2 exposure and TGF-beta-induced EMT on side population (SP) cells of breast cancer cell lines. The results showed contrasting effects on SP cells, suggesting the involvement of ER(x) expression and TGFB-RII in regulating SP cell behavior.
Review
Cell Biology
Simon G. Powell, Priyanka Sharma, Samuel Masterson, James Wyatt, Ilyas Arshad, Shakil Ahmed, Gendie Lash, Michael Cross, Dharani K. Hapangama
Summary: Deep endometriosis (DE) is the most severe subtype of endometriosis, characterized by lesions infiltrating adjacent tissue. Abnormal vascularization is implicated in DE lesion development, with higher microvessel density (MVD) and increased levels of vascular endothelial growth factor (VEGF-A) and its associated receptor (VEGFR-2) in DE lesions compared to other types of endometriotic lesions. Progestin therapy decreases MVD in DE lesions, suggesting potential therapeutic targets for DE management. This systematic review provides comprehensive evidence of the role of abnormal vascularization in DE pathogenesis.
Article
Public, Environmental & Occupational Health
Huijia Lin, Xiaoya Wang, Shuang Qin, Fanglan Luo, Yingmei Cen, Gendie E. Lash, Li Li
Summary: This study investigated the hearing status of Turner Syndrome (TS) patients in China and the influencing factors. It was found that TS patients commonly have mid-frequency and high-frequency hearing loss, and the incidence of hearing loss increases with age. Patients with 45, X haplotype have a higher risk of mid-frequency hearing loss compared to other karyotypes. Therefore, karyotype may serve as a predictor of hearing problems in TS patients.
FRONTIERS IN PUBLIC HEALTH
(2023)
Review
Respiratory System
Emma J. Ragel, Lynda K. Harris, Richard A. Campbell
Summary: ARDS is a severe form of lung injury leading to respiratory failure, with no robust pharmacological treatments available. The optimal treatment for COVID-19 ARDS may differ from ARDS developed from other illnesses due to differences in pathology. New therapeutic interventions developed for CARDS may hold strong potential in treating ARDS developed from other illnesses.
BMJ OPEN RESPIRATORY RESEARCH
(2023)
Article
Developmental Biology
Hanbo Liu, Miaojuan Chen, Fen Ning, Yixin Ye, Qinsheng Lu, Shenjiao Lu, Yaoyun Duan, Xiaowen Gan, Mingguang Zhao, Kaimin Guo, Gendie E. Lash
Summary: During spiral artery remodeling in early pregnancy, extravillous trophoblast (EVT) cells promote vascular smooth muscle cell (VSMC) apoptosis. This study showed that EVT-derived exosomes carrying miR-143-3p and expressing FASL on the cell surface induce VSMC apoptosis, providing insights into the regulation of VSMC apoptosis in spiral artery remodeling.
MOLECULAR HUMAN REPRODUCTION
(2023)
