Effects of Penta-O-galloyl--D-glucose on Human Neutrophil Function: significant Down-Regulation of L-selectin Expression

Penta-O-galloyl--D-glucose (PGG) occurrs in high concentrations in medicinal herbs such as Rhus chinensis, Paeonia suffruticosa, Acer truncatum and Terminalia chebula, which demonstrate anti-inflammatory activity. We investigated the effect of PGG on stimulated and non-stimulated neutrophils in processes which included reactive oxygen species generation (ROS), metalloproteinase-9 and interleukin-8 secretion (IL-8), (2) integrin (CD11b) and L-selectin (CD62L) expression and apoptosis. In concentrations of 5M-20M, PGG demonstrated statistically significant inhibition of ROS generation, IL-8 secretion and (2) integrin expression in stimulated neutrophils. The inhibition of L-selectin expression by PGG resulted in prevention in neutrophils' endothelial attachment. The result obtained may explain the anti-inflammatory activity of this compound and underline the contribution of PGG in the activity of PGG rich plant extracts. Copyright (c) 2012 John Wiley & Sons, Ltd.