Article
Medicine, Legal
Rafaela Castro Dornelles, Camille Gaube Guex, Rachel de Lima, Daniele Rubert Nogueira-Librelotto, Rosana Casoti, Ana Martiele Engelmann, Camila Benaduce Emanuelli Mello, Julia Brandt de Souza, Cinthia Melazzo de Andrade, Alencar Kolinski Machado, Micheli Mainardi Pillat, Melania Palermo Manfron, Liliane de Freitas Bauermann
Summary: This study investigated the phytochemical composition, in vitro and in vivo toxicities, and antiproliferative activity of Richardia brasiliensis. The study identified alkaloids and terpenes as significant compounds in the plant. The results showed that R. brasiliensis had a good antiproliferative effect against human melanoma cells and exhibited no significant toxicity in in vitro and in vivo tests. Further studies on its toxicity at higher concentrations are needed.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Nutrition & Dietetics
Mahasin Abdel Rhman, Nikita Devnarain, Rene Khan, Peter M. O. Owira
Summary: This study reveals that the combination of quercetin and naringenin in citrus fruits and vegetables can synergistically induce oxidative stress and apoptosis, and reduce cell proliferation in breast cancer cells.
Article
Medicine, General & Internal
Lukasz Oldak, Patrycja Milewska, Sylwia Chludzinska-Kasperuk, Kamil Grubczak, Joanna Reszec, Ewa Gorodkiewicz
Summary: This study focused on quantifying cathepsin B, D, and S in gliomas, exploring their correlation with patient age, tumor size, and each other. The research led to the development of a diagnostic method for distinguishing different grades of glioma and identifying independent markers using cathepsins.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Food Science & Technology
Romina L. Ferrero, Carmen Soto-Maldonado, Caroline Weinstein-Oppenheimer, Zaida Cabrera-Munoz, Maria Elvira Zuniga-Hansen
Summary: The study aimed to obtain an enzymatic hydrolysate of rapeseed protein that inhibits proliferation of breast cancer cells, while not affecting healthy human fibroblast cells. One of the hydrolysates showed anti-proliferative activity on MCF-7 cells, with a composition mainly consisting of 10 kDa peptides. This particular hydrolysate, DH1, has the potential to be a promising peptide extract for future research as a possible anti-breast cancer agent.
Article
Biochemistry & Molecular Biology
Kazunari Tanigawa, Mitsuo Kiriya, Yasuhiro Hayashi, Yoshiaki Shinden, Yuko Kijima, Shoji Natsugoe, Takahiro Sumimoto, Riyo Morimoto-Kamata, Satoru Yui, Kotaro Hama, Kazuaki Yokoyama, Yasuhiro Nakamura, Koichi Suzuki, Hisao Nojiri, Keizo Inoue, Ken Karasawa
Summary: This study found that cathepsin G (Cat G), a protease highly expressed in breast cancer cells, induces cell migration and aggregation. Platelet-activating factor (PAF)-acetylhydrolase (PAF-AH), an enzyme responsible for PAF degradation, is involved in Cat G-induced aggregation and migration of breast cancer cells. Inhibition of PAFAH1B2 suppresses Cat G-induced migration and aggregation. These findings may contribute to the development of novel therapeutic strategies for breast cancer treatment.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Chemistry, Multidisciplinary
Yildiz Bal, Yusuf Surmeli, Gulsah Sanli-Mohamed
Summary: Alginate microcapsules loaded with olive leaf extract (OLE) were immobilized using calcium alginate (CA) and chitosan-coated CA (CCA) in this study. The loading capacity of OLE-CA and OLE-CCA was found to be 80% and 99%, respectively. Cytotoxicity analysis showed that OLE-CA and OLE-CCA exhibited higher cytotoxic effects against A549 and MCF-7 cells compared to free OLE. Optical microscopy images confirmed the greater effectiveness of OLE microcapsules against these cancer cells.
Article
Pharmacology & Pharmacy
Sholpan Nurzhan, Zhibek Bekezhankyzy, Hong Ding, Nurken Berdigaliyev, Shynggys Sergazy, Alexander Gulyayev, Zarina Shulgau, Christopher R. Triggle, Mohamad Aljofan
Summary: The study reveals that the glucose-lowering drug metformin has anticancer properties, but its effectiveness and mechanism may vary depending on the glucose environment. The concentration of glucose can affect the antiproliferative potency and cellular mechanism of action of metformin.
Editorial Material
Medicine, General & Internal
Luca Pipito, Antonio Cascio
Summary: A 75-year-old woman developed painful nodules on her right hand and forearm for 10 days after being punctured by a thorn. Bacterial culture was performed on the wound aspirate.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Chemistry, Medicinal
Joyce E. Gutierrez, Esteban Fernandez-Moreira, Miguel A. Rodriguez, Michael R. Mijares, Juan Bautista De Sanctis, Sona Gurska, Petr Dzubak, Marian Hajduch, Julia Bruno-Colmenarez, Luis Rojas, Denis Deffieux, Laurent Pouysegu, Stephane Quideau, Jaime Charris, Hegira Ramirez
Summary: Some sulfanyl and sulfinyl derivatives exhibited lower cytotoxicity for cancer cell lines compared to sulfonyl N-oxide derivatives. Compound 81 showed pronounced selectivity for human colorectal cancer cells and leukemia cell lines.
Article
Chemistry, Medicinal
Mariam M. Fakhry, Kazem Mahmoud, Mohamed S. Nafie, Ahmad O. Noor, Rawan H. Hareeri, Ismail Salama, Safaa M. Kishk
Summary: In this study, a novel series of EGFR/HER2 dual inhibitors were designed and synthesized, which showed strong anti-proliferative activity against MCF-7 breast cancer cells and were validated for their enzyme activity. Additionally, these compounds promoted apoptotic cell death in breast cancer cells, demonstrating their potential as anticancer agents.
Article
Biochemistry & Molecular Biology
Mohammed Aufy, Ramadan F. Abdelaziz, Ahmed M. Hussein, Nermina Topcagic, Hadil Shamroukh, Mostafa A. Abdel-Maksoud, Tamer Z. Salem, Christian R. Studenik
Summary: This study investigated the mechanism of cell death induced by Enniatin B (ENN B) and Beauvericin (BEA). The results showed that these compounds increased the extracellular secretion of cathepsin B and reduced lysosomal staining. Inhibition of cathepsin B significantly reduced the cytotoxic effect of both compounds, while inhibition of cathepsin D or cathepsin L did not influence their cytotoxic activities. Furthermore, the labeling of lysosomal cysteine cathepsins was significantly reduced for cathepsin B but not for cathepsin L upon treatment with these compounds. Therefore, ENN B and BEA increase lysosomal pH, inhibit the delivery of cathepsin B to lysosomes, and induce its release into the cytosol, activating caspases and the apoptotic pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Jingzhe Song, Nan Zhu, Xinchen Pan, Lu Guo, Xiang Kong
Summary: This study investigated the expression and significance of Cathepsin C (Cat C) and Cathepsin D (Cat D) in the serum and placenta of nonpregnant women, pregnant women in different trimesters, and patients with preeclampsia (PE). The results showed that the levels of Cat C were significantly lower in the first trimester compared to the nonpregnant group, while Cat D levels were significantly higher. Cat C and D levels increased in the second and third trimesters, with no significant difference between the two trimesters. In patients with PE, Cat C levels were significantly higher and Cat D levels were significantly lower than in the third trimester, and these levels were correlated with the severity of PE.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Chemistry, Applied
Alexander M. Scherbakov, Olga V. Stasevich, Diana I. Salnikova, Olga E. Andreeva, Ekaterina I. Mikhaevich
Summary: Research has shown that secoisolariciresinol diglucoside (SDG) and its derivatives exhibit inhibitory effects on cell growth and promotion of apoptosis in breast cancer cells, with significant impact on estrogen receptor activity.
NATURAL PRODUCT RESEARCH
(2021)
Article
Multidisciplinary Sciences
Alma Alicia Ortiz-Morales, Juan Benjamin Garcia-Vazquez, Manuel Jonathan Fragoso-Vazquez, Martha Cecilia Rosales-Hernandez, Leticia Guadalupe Fragoso-Morales, Alan Ruben Estrada-Perez, Jose Correa-Basurto
Summary: Our work group synthesized a promising compound named N-(2-hydroxyphenyl)-2-propylpentanamide (HO-AAVPA), which acts as a HDAC1 inhibitor and exhibits antiproliferative properties in cancer cell lines. However, HO-AAVPA has poor water solubility and is metabolized by enzymes. In this study, we used a fourth-generation poly(amidoamine) dendrimer (PAMAM-G4) as a drug delivery carrier for HO-AAVPA. We also evaluated the effects of the HO-AAVPA-PAMAM-G4 complex on forced degradation and antiproliferation in breast cancer cells (MCF-7). The results showed that PAMAM-G4 protected HO-AAVPA from degradation and increased its water solubility, leading to enhanced antiproliferative effects in MCF-7 cells.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Sandeep Goyal, Ketul V. Patel, Yadav Nagare, Digambar B. Raykar, Santosh S. Raikar, Atul Dolas, Princy Khurana, Rajath Cyriac, Sharad Sarak, Mukesh Gangar, Anil K. Agarwal, Aditya Kulkarni
Summary: This study describes the design, optimization, and evaluation of novel non-peptidic acylguanidine based small molecule inhibitors of Cathepsin D, leading to the identification of an optimized lead 24e with improved microsomal stability and potency. This compound could serve as a potential candidate for further preclinical studies against diseases where Cathepsin D plays a key role.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Education & Educational Research
Diana Vilela Azzi, Julia Melo, Debora Ribeiro Orlando, Ramiro Mendonca Murata, Paula Midori Castelo, Eric Francelino Andrade, Luciano Jose Pereira
Summary: The study aimed to evaluate the levels of Burnout Syndrome (BS) in Agricultural Sciences students before and during the COVID-19 pandemic. The results showed that academic efficacy improved over time, and physical activity and online teaching strategies were associated with reduced levels of burnout.
JOURNAL OF AGRICULTURAL EDUCATION & EXTENSION
(2023)
Review
Plant Sciences
Gustavo Aparecido da Cunha, Paulo Fernando Carlstrom, Marcelo Franchin, Severino Matias Alencar, Masaharu Ikegaki, Pedro Luiz Rosalen
Summary: This systematic review evaluated the impact of propolis administration in diabetic animals. The study found that propolis administration resulted in a hypoglycemic effect in the treated animals, which may be attributed to the reduction of oxidative stress. Additionally, propolis administration restored endogenous antioxidant defenses and improved the lipid profile of the treated animals. However, the review also identified limitations in the studies, including a lack of randomization, blinding, and proper allocation of animals.
Article
Chemistry, Applied
Adna Prado Massarioli, Alan Giovanini de Oliveira Sartori, Fernanda Francetto Juliano, Jose Eduardo Pedroso Gomes do Amaral, Roseane Cavalcanti dos Santos, Liziane Maria de Lima, Severino Matias de Alencar
Summary: This study predicts the bioaccessibility and intestinal permeability of major phenolics in peanuts using simulated digestion and Caco-2 cell model. The results show that the phenolics in peanuts undergo changes during digestion, but their antioxidant activity remains unchanged. Furthermore, peanuts are found to contain p-coumaric acid as a major phenolic compound.
Article
Food Science & Technology
Samuel Henrique Gomes Sa, Marcella Chalella Mazzocato, Ana Sofia M. C. Saliba, Severino M. Alencar, Carmen Silvia Favaro-Trindade
Summary: The aim of this study was to microencapsulate red propolis extract using spray-drying, spray-chilling, and their combination. The produced particles effectively protected the bioactive compounds of the propolis extract and exhibited different releasing characteristics. This research provides a range of potential applications in the food, feed, cosmetic, and pharmaceutical industries.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Entomology
Paula Tatiana Lopes Seixas, Antonio Jacinto Demuner, Luiz Claudio Almeida Barbosa, Cristiane Isaac Cerceau, Daiane Einhardt Blank, Marcelo Henrique Dos Santos, Elizeu de Sa Farias, Marcelo Coutinho Picanco
Summary: The larvicidal effect of essential oils from the leaves of three Artemisia species against A. aegypti was evaluated. The EO of Artemisia camphorata was found to be the most effective, with germacrene D-4-ol as the main active constituent. It can be promising for developing natural larvicides against A. aegypti.
JOURNAL OF APPLIED ENTOMOLOGY
(2023)
Article
Medicine, Research & Experimental
Julianne Caravita Grisolia, Pedro Luiz Rosalen, Lauana Aparecida Santos, Masaharu Ikegaki, Luiz Cosme Cotta Malaquias, Eva Burger
Summary: PCM is a severe mycosis caused by Paracoccidioides brasiliensis, mainly found in tropical countries. Itraconazole has marked antifungal activity against Pb and can activate both innate and acquired immunity. The effect of Itraconazole on Pb infection varies with different doses, with lower doses enhancing immune response and higher doses not requiring immune response.
Article
Genetics & Heredity
Simara Larissa Fanalli, Bruna Pereira Martins Silva, Julia Dezen Gomes, Mariah Castro Durval, Vivian Vezzoni de Almeida, Gabriel Costa Monteiro Moreira, Barbara Silva-Vignato, Juliana Afonso, Felipe Andre Oliveira Freitas, James Mark Reecy, James Eugene Koltes, Dawn Koltes, Dorian Garrick, Luciana Correia de Almeida Regitano, Julio Cesar de Carvalho Balieiro, Gerson Barreto Mourao, Luiz Lehmann Coutinho, Heidge Fukumasu, Severino Matias de Alencar, Albino Luchiari Filho, Aline Silva Mello Cesar
Summary: Pigs are used as an animal model for studying metabolic diseases in humans. This study investigated the effects of different types of oils (canola, fish, and soybean oils) on pig gene expression and identified the impacted metabolic pathways. The results provide insights into the biological mechanisms underlying the effects of dietary oils on gene expression in pigs.
FRONTIERS IN GENETICS
(2023)
Article
Biotechnology & Applied Microbiology
Bruna Pereira Martins da Silva, Simara Larissa Fanalli, Julia Dezen Gomes, Vivian Vezzoni de Almeida, Heidge Fukumasu, Felipe Andre Oliveira Freitas, Gabriel Costa Monteiro Moreira, Barbara Silva-Vignato, James Mark Reecy, James Eugene Koltes, Dawn Koltes, Julio Cesar de Carvalho Balieiro, Severino Matias de Alencar, Julia Pereira Martins da Silva, Luiz Lehmann Coutinho, Juliana Afonso, Luciana Correia de Almeida Regitano, Gerson Barreto Mourao, Albino Luchiari Filho, Aline Silva Mello Cesar
Summary: This study evaluated the effect of different levels of soybean oil on lipid metabolism and transcriptome in pig's brain tissue. The results showed that soybean oil levels did not affect brain lipid content and fatty acid profile, but there were changes in gene expression related to intracellular calcium, lipid metabolism, and oxidative processes.
Article
Chemistry, Applied
Ana Sofia Martelli Chaib Saliba, Alan Giovanini de Oliveira Sartori, Pollyanna Souza Batista, Jose Eduardo Pedroso Gomes do Amaral, Namuhell Oliveira da Silva, Masaharu Ikegaki, Pedro Luiz Rosalen, Severino Matias de Alencar
Summary: This study aimed to investigate the impact of gastrointestinal digestion/Caco-2 cell transport on the biological activities and toxicity of ethanolic extract of organic propolis from southern Brazil (EEOP1). The results demonstrated that EEOP1 deactivated reactive oxygen species and attenuated the activation of transcription factors, while also reducing the release of pro-inflammatory cytokines. Additionally, the phytochemical profile of EEOP1 changed after digestion, and antimicrobial activity was only observed in the non-digested form.
Article
Chemistry, Applied
Severino Matias de Alencar, Alan Giovanini de Oliveira Sartori, Damla Dag, Pollyanna Souza Batista, Pedro Luiz Rosalen, Masaharu Ikegaki, Fanbin Kong
Summary: The effect of dynamic gastrointestinal digestion/Caco-2 cell transport on the stability and activities of Brazilian red propolis extract (EEBRP) was investigated. Encapsulated EEBRP showed higher bioaccessibility and nonencapsulated EEBRP had higher Caco-2 cell transport. Nonencapsulated EEBRP exhibited higher ability to scavenge reactive oxygen species and decrease inflammation markers after cell transport. Further studies on encapsulation are encouraged for improving bioactivity and expanding food applications.
Article
Agriculture, Multidisciplinary
Fernanda Papa Spada, Josy Goldoni Lazarini, Pollyanna Souza Batista, Alan Giovanini de Oliveira Sartori, Ana Sofia Martelli Chaib Saliba, Jose Eduardo Pedroso Gomes do Amaral, Eduardo Purgatto, Severino Matias de Alencar
Summary: The in vivo toxicity and in vitro antioxidant activity of fermented jackfruit seed flour (Fjs) and non-alkaline cocoa powder (Nac) were investigated in this study. The results showed that there were no significant differences in the antioxidant activity of Nac-D and Fjs-D after simulated gastrointestinal digestion. Furthermore, both Fjs and Nac exhibited beneficial effects in terms of cellular antioxidant activity and anti-inflammatory assay.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Horticulture
Adna Prado Massarioli, Severino Matias de Alencar, Adriano Francisco Siqueira, Mariana Pereira de Melo, Igor Gomes Vidigal, Ana Lucia Gabas Ferreira
Summary: This study evaluates the effects of drying methods on the drying rates, characteristics, and antioxidant properties of five herbs. Forced-air oven drying resulted in higher drying rates compared to solar drying. Mint was less affected by drying conditions, while all dried herbs exhibited similar antioxidant properties. Standardization of post-harvest steps is important for maintaining the consistency of herbal products.
Article
Chemistry, Applied
Gabriela Boscariol Rasera, Francielle Miranda de Matos, Anna Paula de Souza Silva, Severino Matias de Alencar, Raquel Bridi, Adriano Costa de Camargo, Ruann Janser Soares de Castro
Summary: Germination has been found to improve the antioxidant properties of mustard by increasing the bio-accessibility of phenolic compounds. In this study, the in vitro digestion process was simulated to evaluate the impact of germination on mustard phenolic bio-accessibility. Liquid chromatography-mass spectrometry analysis identified resveratrol, formononetin, and cryptochlorogenic acid as key phenolic compounds. Results showed that digestion positively affected the antioxidant potential of soluble phenolics, although it appeared to negate the improvement caused by germination. However, germinated digested mustard had significantly higher concentrations of sinapic acid compared to non-germinated.
Article
Food Science & Technology
Gisele Goulart da Silva, Lucia Elaine de Oliveira Braga, Ellen Cristina Souza de Oliveira, Joao Ernesto de Carvalho, Josy Goldoni Lazarini, Pedro Luiz Rosalen, Ana Paula Dionisio, Ana Lucia Tasca Gois Ruiz
Summary: The standardized extract (CAE) obtained from cashew apple bagasse shows potential beneficial effects on ulcerative colitis. CAE is more effective in delaying disease progression but cannot prevent or inhibit the disease. The concentration of carotenoids in CAE may not be sufficient, and there may be an antagonistic relationship between carotenoids and anacardic acids.
Article
Chemistry, Applied
Marcelo Franchin, Ana Sofia Martelli Chaib Saliba, Alan Giovanini de Oliveira Sartori, Sebastiao Orestes Pereira Neto, Bruna Benso, Masaharu Ikegaki, Kai Wang, Severino Matias de Alencar, Daniel Granato
Summary: Brazilian propolis from Apis mellifera is widely studied for its unique chemical composition and biological properties. This review explores the potential uses of propolis as a functional ingredient in food and pharmaceutical applications, providing a theoretical and practical basis for optimizing its bioactive properties.
