Journal
PHYTOTHERAPY RESEARCH
Volume 23, Issue 7, Pages 920-926Publisher
WILEY
DOI: 10.1002/ptr.2763
Keywords
seasonal allergies; nettle; DART TOF-MS; mast cell degranulation
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Funding
- HerbalScience Group LLC
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A nettle (Urtica dioica) extract shows in vitro inhibition of several key inflammatory events that cause the symptoms of seasonal allergies. These include the antagonist and negative agonist activity against the Histamine-1 (H-1) receptor and the inhibition of mast cell tryptase preventing degranulation and release of a host of pro-inflammatory mediators that cause the symptoms of hay fevers. The nettle extract also inhibits prostaglandin formation through inhibition of Cyclooxygenase-1 (COX-1), Cyclooxygenase-2 (COX-2), and Hematopoietic Prostaglandin D-2 synthase (HPGDS), central enzymes in pro-inflammatory pathways. The IC50 value for histamine receptor antagonist activity was 251 (+/-13) mu g mL(-1) and for the histamine receptor negative agonist activity was 193 (+/-71) mu g mL(-1). The IC50 values for inhibition of mast cell tryptase was 172 (+/-28) mu g mL(-1), for COX-1 was 160 (+/-47) mu g mL(-1), for COX-2 was 275 (+/-9) mu g mL(-1), and for HPGDS was 295 (+/-51) mu g mL(-1). Through the use of DART TOF-MS, which yields exact masses and relative abundances of compounds present in complex mixtures, bioactives have been identified in nettle that contribute to the inhibition of pro-inflammatory pathways related to allergic rhinitis. These results provide for the first time, a mechanistic understanding of the role of nettle extracts in reducing allergic and other inflammatory responses in vitro. Copyright (C) 2009 John Wiley & Sons, Ltd.
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