Journal
PHYTOMEDICINE
Volume 21, Issue 3, Pages 298-306Publisher
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2013.08.016
Keywords
Neuroinflammation; Microglia; Sesquiterpene dimer; Signaling pathway
Categories
Funding
- National Key Technology R&D Program New Drug Innovation of China [2012ZX09301002-002-002]
- Peking-Tsinghua Center for Life Sciences
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Microglia-involved neuroinflammation is thought to promote brain damage in various neurodegenerative disorders. Therefore, novel therapeutics suppressing microglia over-activation could prove useful for neuroprotection in inflammation-mediated neurodegenerative diseases. DSF-52 is a novel sesquiterpene dimer compound isolated from medical plant Artemisia argyi by our group. In this study, we investigated whether DSF-52 inhibited the neuroinflammatory responses in lipopolysaccharide (LPS)-activated microglia. Our findings showed that DSF-52 inhibited the production of nitric oxide (NO), prostaglandin E-2 (PGE(2)), tumor necrosis factor-alpha (TNF-alpha), as well as mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), interleukin-1 beta (IL-1 beta), granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage inflammatory protein-1 alpha (MIP-1 alpha) in LPS-activated BV-2 microglia. Moreover, DSF-52 markedly up-regulated mRNA levels of anti-inflammatory cytokine IL-10 Mechanism study indicated that DSF-52 suppressed Akt/I kappa B/NF-kappa B inflammation pathway against LPS treatment. Also, DSF-52 down-regulated the phosphorylation levels of JNK and p38 MAPKs, but not ERK. Furthermore, DSF-52 blocked Jak2/Stat3 dependent inflammation pathway through inhibiting Jak2 and Stat3 phosphorylation, as well as Stat3 nuclear translocation. We concluded that the inhibitory ability of DSF-52 on microglia-mediated neuroinflammation may offer a novel neuroprotective modality and could be potentially useful in inflammation-mediated neurodegenerative diseases. (C) 2013 Elsevier GmbH. All rights reserved.
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