Journal
PHYTOMEDICINE
Volume 18, Issue 2-3, Pages 219-223Publisher
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2010.07.002
Keywords
Cardiospermum halicacabum; Cardiospermin; Anxiolytic effect; Elevated plus-maze test; Light dark model
Categories
Funding
- PES College of Pharmacy
- Manipal University
Ask authors/readers for more resources
Cardiospermum halicacabum roots have been used traditionally for the treatment of epilepsy and anxiety disorders. The purpose of this study was to characterize the putative phytoconstituents present in the ethanolic root extract having anxiolytic activity using an elevated plus-maze (EPM) and light dark transition model. Control mice were orally treated with an equal volume of vehicle (4% gum acacia), and positive control mice were treated with diazepam (1 mg/kg). In the EPM test, out of pool of 19 master fractions (MF) only MF-14, 16 and 17 significantly (30 mg/kg, p < 0.05, p < 0.01 and p < 0.001) increased the number of entries in the open arm. MF-14, 16 and 17 (10,20 and 30 mg/kg) had also increased the time spent by mice in illuminated part of the box significantly (p < 0.05, p < 0.01 and p < 0.001), as compared to control. However, significant changes (p < 0.05. p < 0.01 and p < 0.001) were recorded in other parameters, e.g., rearing, time spent in the closed arm and dark zone in both the models. These results suggested that C. halicacabum root is an effective anxiolytic agent. The phytoconstituent responsible for the observed central effects was isolated from MF-14 and identified as well-known compound, Cardiospermin, a cyanogenic glucoside. (C) 2010 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available