Journal
PHYTOMEDICINE
Volume 16, Issue 4, Pages 327-335Publisher
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2008.12.017
Keywords
Tanshinones; Salvia miltiorrhiza; Insulin receptor tyrosine phosphorylation; Insulin signal transduction; Glucose transporter; 3T3-L1-adipocytes
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Funding
- KFDA through the NCSHM [R04-2004-000-10077-0]
- Korea Research Foundation
- NCRC Program [R15-2006-020]
- MEST
- KOSEF through the Center for Cell Signaling & Drug Discovery Research at Ewha Womans University
- Yangyoung Foundation
- Korea Ministry of Education
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In this study, the effects of the extract and four tanshinone compounds from the dried root of Salvia miltiorrhiza Bunge (Labiatae) on the tyrosine phosphorylation of the insulin receptor (IR.) beta-subunit and the downstream signaling were examined it, Chinese-hamster ovary cells expressing human insulin receptors (CHO/IR cells) as well as in 3T3-L1 adipocytes. In addition the translocation of the glucose transporter 4 was investigated in 3T3-L1 adipocytes. Total extract of Danshen (1-10 mu g/ml) and the four tanshinones (10 mu M) did not show any activity, but the total extract and the tanshinone I, IIA and 15, 16-dihydrotanshinone I except cryptotanshinone enhanced the activity of insulin (1 nM) on the tyrosine phosphorylation of the IR as well as the activation of the downstream kinases Akt, ERK1/2, and GSK3 beta. In the adipocytes the same IR-downstream signaling and the translocation of glucose transporter 4 were demonstrated by the three tanshinones in the presence of insulin. These insulin-sensitizing activities of tanshinones may be useful for developing a new class of specific IR activators as anti-diabetic agents. (C) 2008 Elsevier GmbH. All rights reserved.
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