Journal
PHYTOCHEMISTRY LETTERS
Volume 8, Issue -, Pages 73-76Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.phytol.2014.02.003
Keywords
Diplopterygium rufopilosum; Gleicheniaceae; Ecdysteroids; Antibacterial activity; Antifungal activity
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Funding
- Scientific Planning Project of the Applied Basic Research of Yunnan Province [S2012FZ0005]
- Key Projects of Scientific Research Foundation of the Department of Education of Yunnan Province [2013Z095]
- Key Projects in Scientific Research of Qujing Normal University [2011ZD003]
- Developing Key Subject of Ecology of Qujing Normal University
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Phytochemical investigation of the ethanol extract of Diplopterygium rufopilosum resulted in the isolation of three new ecdysteroids, (22R,24R,25S,26S)-2 beta,3 beta,14 alpha,20R-tetrahydroxy-26 alpha-methoxy-6-oxo-stigmast-7-ene-22,26-lactone (1), (22R, 24R, 25S)-2 beta,3b,14a,20R,26S-pentahydroxy-6-oxo-stigmast-7-ene-22,26-lactone (2), and (22R, 25S)-2 beta,3 beta, 14 alpha,20R,24S-pentahydroxy-6,26-dioxo-stigmast-7-ene-22,26-lactone (3), together with two known compounds (4) and (5). Their structures were determined on the basis of spectroscopic analyses, including 1D-NMR, 2D-NMR, and HR-ESI-MS. The isolated ecdysteroids were evaluated in vitro for antimicrobial properties, and exhibited moderate antibacterial activities against the tested oral pathogens. (C) 2014 Published by Elsevier B.V. on behalf of Phytochemical Society of Europe.
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