Article
Biochemistry & Molecular Biology
Natalia Ritter Ruas, Aline Carvalho Pereira, Luciana Lopes Silva Pereira, Carolina Mesquita Germano, Elaine Fontes Ferreira da Cunha, Alexandre Alves de Carvalho, Osmar Alves Lameira, Jose Eduardo Brasil Pereira Pinto, Suzan Kelly Vilela Bertolucci
Summary: This study aimed to quantify verbascoside (VEB), perform molecular docking studies of VEB with the alpha-glucosidase (GL) of Bacillus stearothermophilus, and evaluate the inhibition of the enzyme by L. dulcis preparations. It was found that antidiabetic fractions 2 and 3 showed the strongest inhibition of the GL enzyme, but their activity decreased significantly in the presence of gastric fluid. Chromatographic analyses confirmed the presence of VEB in the samples, with concentrations ranging from 49.9 to 243.5 mg/g. Molecular docking simulations supported the GL-inhibitory activity of VEB. Overall, crude ethanol extract and fractions demonstrated inhibitory activity against the GL enzyme.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Food Science & Technology
Roghayeh Siahbalaei, Gholamreza Kavoosi, Mostafa Noroozi
Summary: Microalgae proteins are new sources of functional amino acids with nutritional value comparable to conventional protein sources, and their amino acid extracts exhibit anti-amylase and anti-glucosidase properties. Kinetic and molecular docking analyses show that these amino acids inhibit enzyme activity through non-competition and un-competition strategies.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Xuelian Qi, Junlan Shao, Yinchu Cheng, Xiaoying He, Yan Li, Honghua Jia, Ming Yan
Summary: An alpha-glucosidase from Oryza sativa was heterologously produced in Pichia pastoris and used for the efficient biosynthesis of AA-2G. The purified enzyme displayed high specific activity at 37 degrees C and pH 4.0, and successfully synthesized AA-2G under specific conditions. These studies provide a foundation for the industrial application of alpha-glucosidase.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Hidayat Hussain, Mamona Nazir, Muhammad Saleem, Ahmed Al-Harrasi, Elizbit, Ivan R. Green
Summary: This article discusses the effects of various metabolites isolated from fungi on alpha-glucosidase and their potential as anti-diabetic agents.
PHYTOCHEMISTRY REVIEWS
(2021)
Article
Food Science & Technology
Md Badrul Alam, Jeong-Sic Ra, Ji-Young Lim, Bo-Rim Song, Ahsan Javed, Sang-Han Lee
Summary: The study found that lariciresinol (LSR) has an antidiabetic effect. LSR inhibits the activity of α-glucosidase and promotes glucose uptake by activating insulin signaling. In diabetic mice, LSR treatment significantly reduces blood glucose levels, increases insulin levels, improves pancreatic islet size, enhances insulin signaling in skeletal muscle, and activates glycogen synthase kinase 3 beta (GSK-3 beta) to increase glycogen content. These findings suggest a potential usefulness of oral LSR in the management and prevention of diabetes.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Chemistry, Physical
Mohamed M. Khalifa, Helmy M. Sakr, Albaraa Ibrahim, Ahmed M. Mansour, Rezk R. Ayyad
Summary: A series of benzylidene-quinazolinone hybrids were synthesized and evaluated for their alpha-glucosidase inhibitory effect, with five compounds demonstrating potent anti-hyperglycemic activities. Among them, compounds 10(m) and 10(f) showed the most potent inhibitory activities towards alpha-glucosidase.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Biochemical Research Methods
Shao-Yan Zheng, Wei-Jie Zhou, Xiang-Na Lin, Fei-Fei Li, Chun-Fang Xie, Da-Ling Liu, Dong-Sheng Yao
Summary: This study introduces a rational molecular design strategy to regulate enzymatic reactions based on inhibiting the formation of ground state of enzyme-substrate complex. The mutant Y215W was obtained by analyzing the molecular docking binding energy and hydrogen bond formation between AG and the substrates. Compared with the wild-type, the mutant showed improved affinity towards AA-2G and increased synthesis efficiency.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Integrative & Complementary Medicine
Li Yang, Junwei He
Summary: Compounds 1, 4-6, 15, and 17 from the flower of Hosta plantaginea have anti-inflammatory effects by blocking the NF-κB signaling pathway and suppressing the overproduction of inflammatory mediators, which may be helpful in managing inflammatory diseases.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2022)
Article
Biochemistry & Molecular Biology
Ying Zhang, Yanna Pan, Jiaqi Li, Zeng Zhang, Yanming He, Hongjie Yang, Ping Zhou
Summary: This study investigated the inhibitory effect of an anti-oxidative proteoglycan FYGL extracted from Ganoderma lucidum on alpha-glucosidase and non-enzymatic glycation. It was found that FYGL reduced alpha-glucosidase activity by forming a FYGL-alpha-glucosidase complex and inhibited glycation at every stage. In addition, FYGL demonstrated its potential to alleviate postprandial hyperglycemia and reduce diabetic complications. These findings suggest that FYGL could be a valuable therapeutic option for diabetes management.
Article
Cell Biology
Roberto Mikio Kassuya, Elisangela dos Santos, Fernando Henrique Bosso, Taise Fonseca Pedroso, Jane Vasconcelos Neves Marinho, Marcos Jose Salvador, Candida Aparecida Leite Kassuya, Arquimedes Gasparotto Junior
Summary: The study confirmed the anti-inflammatory properties of ethanolic extract from Alternanthera tenella Colla (Amaranthaceae) whole plant and the flavone C-glycoside A19, showing significant inhibitory effects on edema, hyperalgesia, leukocyte migration, and enzyme activities associated with inflammation. Additionally, in silico analysis indicated the potential of A19 as a TNF-alpha inhibitor, further supporting its anti-inflammatory effects.
Article
Infectious Diseases
David O. Hamilton, Tosin Lambe, Alexander Howard, Patricia Crossey, Jennifer Hughes, Rui Duarte, Ingeborg D. Welters
Summary: This study compared the cost of the current diagnostic and treatment pathway for invasive fungal infection (IFI) with a proposed pathway that incorporates Beta-D-Glucan (BDG) testing, from the perspective of the NHS. The results showed that implementing BDG tests in the diagnostic and treatment pathway of patients with suspected IFI could lead to an average saving of £1643 per patient if the test result is obtained within 2 days.
Article
Chemistry, Medicinal
Nguyen Thi Kim Giang, Nguyen Dinh Thanh, Tran Ha Quyen, Doan Thi Huong, Vu Ngoc Toan, Hoang Thi Kim Van
Summary: Several substituted isatin N-(2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl)thiosemicarbazones were synthesized and evaluated for their antibacterial and antifungal activity. Compound 6h exhibited the highest inhibitory activity against Staphylococcus aureus, and docking studies suggested that it inhibited the DNA Gyrase enzyme in S. aureus.
MEDICINAL CHEMISTRY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Lu Bai, Jonghoon Kim, Kwang-Hee Son, Dong-Ha Shin, Bon-Hwan Ku, Do Young Kim, Ho-Yong Park
Summary: Endo-beta-1,3-glucanase from Cellulosimicrobium funkei HY-13 was found to have high activity in degrading laminarin and displayed strong growth inhibition against Candida albicans. Deleting the RICIN domain in the enzyme resulted in a significant decrease in its ability to degrade beta-1,3-D-glucans. The findings suggest that this enzyme could be potentially utilized as a supplementary agent in the food and pharmaceutical industries due to its superior anti-fungal activity.
Article
Chemistry, Multidisciplinary
Soheila Esmaili, Ahmad Ebadi, Ardeshir Khazaei, Hamideh Ghorbani, Mohammad Ali Faramarzi, Somayeh Mojtabavi, Mohammad Mahdavi, Zahra Najafi
Summary: A novel series of pyrano[3,2-c]quinoline-1,2,3-triazole hybrids were synthesized and evaluated for their inhibitory activity against α-glucosidase. Compound 8k exhibited the best inhibitory effect with a competitive pattern of inhibition. Molecular docking and dynamics simulations revealed that the R-enantiomer of compound 8k formed a more stable complex with higher binding affinity to the active site of α-glucosidase compared to the S-enantiomer.
Article
Biology
Sofija Jovanovic Stojanov, Ana Kostic, Mila Ljujic, Ema Lupsic, Silvia Schenone, Milica Pesic, Jelena Dinic
Summary: Drug resistance is a major challenge in treating glioblastoma. Autophagy plays a crucial role in drug resistance, and the use of autophagy inhibitors can enhance the effectiveness of targeted therapy. This study shows that two Src tyrosine kinase inhibitors can induce autophagy in glioblastoma cells, and inhibiting autophagy can increase the anticancer effects of these inhibitors.
Article
Chemistry, Organic
Frederique Deiss, Yang Yang, Wadim L. Matochko, Ratmir Derda
ORGANIC & BIOMOLECULAR CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Simon Ng, Mohammad R. Jafari, Wadim L. Matochko, Ratmir Derda
ACS CHEMICAL BIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Mohammad R. Jafari, Lu Deng, Pavel I. Kitov, Simon Ng, Wadim L. Matochko, Katrina F. Tjhung, Anthony Zeberoff, Anastasia Elias, John S. Klassen, Ratmir Derda
ACS CHEMICAL BIOLOGY
(2014)
Article
Chemistry, Multidisciplinary
Frederique Deiss, Wadim L. Matochko, Natasha Govindasamy, Edith Y. Lin, Ratmir Derda
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2014)
Article
Mathematical & Computational Biology
Wadim L. Matochko, Ratmir Derda
COMPUTATIONAL AND MATHEMATICAL METHODS IN MEDICINE
(2013)
Article
Biochemical Research Methods
Wadim L. Matochko, Simon Ng, Mohammad R. Jafari, Joseph Romaniuk, Sindy K. Y. Tang, Ratmir Derda
Article
Biochemical Research Methods
Wadim L. Matochko, Kiki Chu, Bingjie Jin, Sam W. Lee, George M. Whitesides, Ratmir Derda
Review
Biochemistry & Molecular Biology
Ratmir Derda, Sindy K. Y. Tang, S. Cory Li, Simon Ng, Wadim Matochko, Mohammad R. Jafari
Article
Biochemistry & Molecular Biology
Wadim L. Matochko, S. Cory Li, Sindy K. Y. Tang, Ratmir Derda
NUCLEIC ACIDS RESEARCH
(2014)
Article
Multidisciplinary Sciences
Benjamin M. Scott, Wadim L. Matochko, Richard F. Gierczak, Varsha Bhakta, Ratmir Derda, William P. Sheffield
Article
Medicine, Research & Experimental
Yanli Yang, Sherry H. Yeh, Shravan Madireddi, Wadim L. Matochko, Chen Gu, Patricia Pacheco Sanchez, Mark Ultsch, Gladys De Leon Boenig, Seth F. Harris, Brandon Leonard, Suzie J. Scales, Jing W. Zhu, Erin Christensen, Julie Q. Hang, Randall J. Brezski, Scot Marsters, Avi Ashkenazi, Siddharth Sukumaran, Henry Chiu, Rafael Cubas, Jeong M. Kim, Greg A. Lazar
Correction
Multidisciplinary Sciences
Benjamin M. Scott, Wadim L. Matochko, Richard F. Gierczak, Varsha Bhakta, Ratmir Derda, William P. Sheffield
Article
Cell Biology
Andrew C. Hunt, James Brett Case, Young-Jun Park, Longxing Cao, Kejia Wu, Alexandra C. Walls, Zhuoming Liu, John E. Bowen, Hsien-Wei Yeh, Shally Saini, Louisa Helms, Yan Ting Zhao, Tien-Ying Hsiang, Tyler N. Starr, Inna Goreshnik, Lisa Kozodoy, Lauren Carter, Rashmi Ravichandran, Lydia B. Green, Wadim L. Matochko, Christy A. Thomson, Bastian Vogeli, Antje Kruger, Laura A. VanBlargan, Rita E. Chen, Baoling Ying, Adam L. Bailey, Natasha M. Kafai, Scott E. Boyken, Ajasja Ljubetic, Natasha Edman, George Ueda, Cameron M. Chow, Max Johnson, Amin Addetia, Mary-Jane Navarro, Nuttada Panpradist, Michael Gale, Benjamin S. Freedman, Jesse D. Bloom, Hannele Ruohola-Baker, Sean P. J. Whelan, Lance Stewart, Michael S. Diamond, David Veesler, Michael C. Jewett, David Baker
Summary: Researchers used a cell-free expression workflow to screen and optimize constructs containing computationally designed miniprotein inhibitors of SARS-CoV-2. They found that a homotrimeric version of the ACE2 mimic AHB2 (TRI2-2) achieved the broadest efficacy. The TRI2-2 miniprotein neutralized Omicron, Delta, and other variants with greater potency than clinically used monoclonal antibodies. It also provided prophylactic and therapeutic protection against SARS-CoV-2 in mice when administered intranasally. The designed miniprotein receptor mimics offer a widely applicable antiviral therapeutic strategy with advantages over antibodies and native receptor traps.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Meeting Abstract
Chemistry, Multidisciplinary
Frederique T. Deiss, Katrina Tjhung, Maribel Funes-Huacca, Simon Ng, Wadim Matochko, Ratmir Derda
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2013)
Meeting Abstract
Chemistry, Multidisciplinary
Wadim L. Matochko, Simon Ng, Mohammad R. Jafari, Sindy K. Y. Tang, Ratmir L. Derda
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2013)
Article
Plant Sciences
Yi Zhu, Junlin Wang, Lu Tang, Yuxin Shang, Weiyi Zhao, Wei Zhang, Ping Hai, Yongbiao Zheng, Wenbo Li, Yanfang Su
Summary: One new iridoid glucoside and two new lignan glycosides, along with three known compounds, were isolated from the whole plants of Lancea tibetica. The structures were determined by spectroscopic methods and X-ray crystallographic analysis. One of the new iridoid glucosides and the known compounds exhibited moderate antioxidant activity.
PHYTOCHEMISTRY LETTERS
(2024)
Article
Plant Sciences
Yi Deng, Minghua Chen, Junming Yi, Yuguo Zheng
Summary: This study successfully designed and synthesized a series of quinazolinone derivatives containing purine molecules, and found that some compounds exhibited strong antiviral activity against tobacco mosaic virus. The findings have important implications for further development of antiviral drugs.
PHYTOCHEMISTRY LETTERS
(2024)
