Article
Forestry
Georgiana Mardare (Balusescu), Liliana Lazar, Teodor Malutan
Summary: Downy thorn-apple Datura innoxia is an invasive plant species containing the important alkaloid scopolamine, and this study investigated the extraction of tropane alkaloids, specifically scopolamine, using different alcoholic solvents, finding that 1-butanol was more effective for extraction.
Article
Plant Sciences
Daniel Schlesinger, Rachel Davidovich Rikanati, Adi Faigenboim, Vera Vendramin, Federica Cattonaro, Moshe Inbar, Efraim Lewinsohn
Summary: D. innoxia seedlings can synthesize tropane alkaloids in their roots and transport them differentially to the shoots.
PHYTOCHEMISTRY LETTERS
(2021)
Article
Plant Sciences
Amany H. A. Abeed, Mohammed Ali, Mamdouh A. A. Eissa, Suzan A. A. Tammam
Summary: The application of sewage water for irrigation improves the growth, yield, and quality of Datura innoxia plants compared to well water irrigation. It increases the content of chlorophyll, carotenoids, and non-enzymatic antioxidants in the plants, as well as improves soil quality.
Article
Chemistry, Applied
Delia Castilla-Fernandez, David Moreno-Gonzalez, Juan F. Garcia-Reyes, Evaristo Ballesteros, Antonio Molina-Diaz
Summary: Datura species are known for their high concentration of tropane alkaloids, which can lead to poisoning when accidentally mixed with edible crops. Testing on frozen spinach and spinach-based infant food products found that some samples had levels close to the maximum limits set by the European Union. This highlights the global concern over the presence of these compounds in various commodities.
Article
Chemistry, Multidisciplinary
Georgiana Mardare (Balusescu), Liliana Lazar, Teodor Malutan
Summary: This study applies spectroscopic methods to identify and detect secondary metabolites in Datura innoxia. The results confirm the presence of important secondary metabolite classes, such as alkaloids, terpenes, phenolics, sterols, and carbohydrates, which are useful in medicine. The study also found the presence of hyoscine in all structural units.
COMPTES RENDUS CHIMIE
(2022)
Review
Plant Sciences
Yuru Wen, Yiran Liao, Yueli Tang, Hongbo Zhang, Jiahui Zhang, Zhihua Liao
Summary: This review discusses the impact of elicitation on the biosynthesis of tropane alkaloids (TAs). It covers single-elicitor treatment, multiple-elicitor treatment, and the combination of elicitation strategy with other strategies. While the synergistic effects of multiple elicitors have been observed, the underlying molecular mechanism remains largely unknown. Further research is needed to effectively implement elicitation strategies in commercial production and explore the integration of elicitation with other strategies.
Article
Agricultural Engineering
Shahla Shameh, Bahman Hosseini, Javier Palazon
Summary: The study demonstrated that using biotechnology and precursor feeding strategies for transgenic engineering of hairy roots in Hyoscyamus reticulatus resulted in higher growth rate, increased content of phenol, flavonoid and alkaloid, and improved antioxidant activity. High levels of hyoscyamine were found in control hairy roots, while scopolamine levels were higher in transgenic hairy roots. The expression of pmt and h6h genes significantly increased after 48 hours in both transgenic and control hairy roots, leading to enhanced accumulation of secondary metabolites in vitro.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Romeo Arago Dougue Kentsop, Roberto Consonni, Michela Alfieri, Marina Laura, Gianluca Ottolina, Iride Mascheretti, Monica Mattana
Summary: This study aims to develop adventitious and hairy roots from Linum lewisi and evaluate their suitability for large-scale production of lignans, including justicidin B. The results indicate that these cultures have higher justicidin B production compared to other Linum species cultures, and the production can be further increased with elicitation.
Article
Chemistry, Applied
Diletta Piatti, Riccardo Marconi, Simone Angeloni, Giovanni Caprioli, Filippo Maggi, Emanuele Ribatti, Vittorio Cattaneo, Gianni Sagratini
Summary: This study developed a method to measure the content of scopolamine and hyoscyamine in medicinal plants and analyzed the content of these alkaloids in different parts of four Solanaceae plants.
NATURAL PRODUCT RESEARCH
(2023)
Article
Chemistry, Applied
Svetlana V. Malysheva, Camille Streel, Mirjana Andjelkovic, Julien Masquelier
Summary: A quantitative UHPLC-MS/MS method was developed for the determination of atropine and scopolamine in various food products. The method was validated and successfully applied in the analysis of different commercial foods. Results showed that only a small percentage of food samples contained atropine and scopolamine, with the highest concentrations found in herbal infusions and spice mixtures.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Biochemistry & Molecular Biology
Zhijun Zhang, Jiahui Zhang, Xiaowei Li, Jinming Zhang, Yunsheng Wang, Yaobin Lu
Summary: The transmission of insect-borne viruses involves intricate interactions between viruses, host plants, and vectors. Chemical compounds play a vital role in these interactions. This study demonstrates that a plant virus infection can alter the chemical composition of its host plant, making it more attractive to its vector and enhancing the vector's biological performance. These findings provide insights into the physiological mechanisms behind the virus's benefits to its vector.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Fumihito Hasebe, Honoka Yuba, Takashi Hashimoto, Kazuki Saito, Nobutaka Funa, Tsubasa Shoji
Summary: This passage discusses the biosynthesis process of tropane alkaloids in the roots of medicinal plants, outlining the key metabolic enzymes and reaction mechanisms involved. The generation of mutants through genome editing techniques demonstrates the impact of PYKS activity loss on tropane alkaloid metabolism, offering valuable insights into the role of scaffold-forming reaction in this biosynthetic pathway.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Anita Sliwinska, Ramona Figat, Anna Zgadzaj, Beata Wilenska, Aleksandra Misicka, Grzegorz Nalecz-Jawecki, Agnieszka Pietrosiuk, Katarzyna Syklowska-Baranek
Summary: In this study, hairy root cultures of the traditional Southeast Asian medicinal plant Polyscias filicifolia were successfully established, and their biosynthetic potential was enhanced through methyl jasmonate elicitation. The hairy root extracts showed significant antigenotoxic and anti-photogenotoxic activities.
Article
Multidisciplinary Sciences
Sabina Velazquez-Marquez, Ivan M. De-la-Cruz, Rosalinda Tapia-Lopez, Juan Nunez-Farfan
Summary: The study analyzed gene divergence of tropane alkaloids and terpene synthases in Datura stramonium and other Solanaceae species, revealing signs of positive selection. The duplication events and differentiation of TAs and TPSs in D. stramonium compared to other Solanaceae suggest their possible involvement in the adaptive evolution of defense against herbivores. Protein modeling and docking showed that the interactions between H6H and ligand in the protein structures obtained from D. stramonium remain consistent with those of D. metel.
Article
Plant Sciences
Zahra Tardast, Alireza Iranbakhsh, Mostafa Ebadi, Zahra Oraghi Ardebili
Summary: This study demonstrates that cold plasma treatment can significantly enhance the biomass and alkaloid production of Datura inoxia calluses by altering gene expression and chromatin structure.
Article
Plant Sciences
Viviana Martins, Marianne Unlubayir, Antonio Teixeira, Arnaud Lanoue, Hernani Geros
Summary: The application of calcium sprays in vineyards has been found to delay fruit maturation, increase fruit firmness, and decrease fruit weight and degrees Brix in 'Loureiro' grapes. Calcium treatment affects amino acid levels, accumulation of caftaric acids, and inhibits anthocyanins accumulation in the berries. Additionally, it promotes specific stilbenoids and flavonols content in the fruit.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Natalja Kulagina, Gregory Guirimand, Celine Melin, Pamela Lemos-Cruz, Ines Carqueijeiro, Johan-Owen De Craene, Audrey Oudin, Vladimir Heredia, Konstantinos Koudounas, Marianne Unlubayir, Arnaud Lanoue, Nadine Imbault, Benoit St-Pierre, Nicolas Papon, Marc Clastre, Nathalie Giglioli-Guivarc'h, Jillian Marc, Sebastien Besseau, Vincent Courdavault
Summary: The pharmaceutical industry is facing increasing demand and recurrent shortages of anticancer plant drugs, prompting the need for efficient alternative strategies such as bioproduction by microorganisms. In this study, yeast cell factories were developed and optimized to efficiently convert tabersonine to vindoline, a precursor to major anticancer alkaloids. Through fine-tuning gene copies and medium optimization, the bioconversion efficiency was significantly improved, resulting in a vindoline titre of 266 mg l(-1) in an optimized fed-batch bioreactor. This precursor-directed synthesis paves the way towards future industrial bioproduction by valorizing abundant tabersonine resources.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Konstantinos Koudounas, Gregory Guirimand, Luisa Fernanda Rojas Hoyos, Ines Carqueijeiro, Pamela Lemos Cruz, Emily Stander, Natalja Kulagina, Jennifer Perrin, Audrey Oudin, Sebastien Besseau, Arnaud Lanoue, Lucia Atehortua, Benoit St-Pierre, Nathalie Giglioli-Guivarc'h, Nicolas Papon, Sarah E. O'Connor, Vincent Courdavault
Summary: Many plant species from the Apocynaceae, Loganiaceae and Rubiaceae families produce a variety of monoterpene indole alkaloids (MIAs) which have pharmacological properties. The subcellular localization of N-methyltransferases (NMTs) in MIA-producing plants adds a new layer of complexity to the biosynthetic pathway.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yohana Laloum, Christophe Gangneux, Bruno Gugi, Arnaud Lanoue, Thibaut Munsch, Adrien Blum, Adrien Gauthier, Isabelle Trinsoutrot-Gattin, Isabelle Boulogne, Maite Vicre, Azeddine Driouich, Karine Laval, Marie-Laure Follet-Gueye
Summary: The study revealed that pea is more susceptible to Aphanomyces euteiches infection compared to faba bean. Pea root exudates showed attraction towards the pathogen, while faba bean exudates exhibited a repellent effect. The research suggests that early belowground defense plays a critical role in host susceptibility to root rot, opening new perspectives for disease management.
Correction
Chemistry, Multidisciplinary
Kevin Billet, Marianne Unlubayir, Thibaut Munsch, Magdalena Anna Malinowska, Thomas Duge de Bernonville, Cecile Marchal, Sandrine Dedet, Audrey Oudin, Vincent Courdavault, Sebastien Besseau, Nathalie Giglioli-Guivarc'h, Arnaud Lanoue
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Plant Sciences
Ines L. Cabral, Antonio Teixeira, Arnaud Lanoue, Marianne Unlubayir, Thibaut Munsch, Joana Valente, Fernando Alves, Pedro Leal da Costa, Frank S. Rogerson, Susana M. P. Carvalho, Hernani Geros, Jorge Queiroz
Summary: This study aimed to analyze the impact of deficit irrigation on vineyards in the Douro Demarcated Region. The results showed that deficit irrigation partially mitigated water deficit conditions and improved yield, but had no significant effect on key metabolites.
Article
Biochemistry & Molecular Biology
Manon Ferrier, Kevin Billet, Samantha Drouet, Duangjai Tungmunnithum, Magdalena Anna Malinowska, Cecile Marchal, Sandrine Dedet, Nathalie Giglioli-Guivarc'h, Christophe Hano, Arnaud Lanoue
Summary: In this study, the polyphenol variations in grape cane extracts from European varieties were analyzed using UPLC-MS-based metabolomics and unsupervised classification. Antioxidant activities were assessed, and pairwise correlations between polyphenols and antioxidant capacities were performed to identify molecules that contributed more to the antioxidant capacities within a complex mixture of polyphenols.
Article
Plant Sciences
Iwaba Kpabi, Thibaut Munsch, Amegninou Agban, Isabelle Thery-Kone, Joelle Dorat, Leslie Boudesocque-Delaye, Pierre-Olivier Delaye, Cedric Neveu, Arnaud Lanoue, Cecile Enguehard-Gueiffier
Summary: This study validated the traditional use of Cassia sieberiana root bark as a dewormer and analyzed the metabolite composition in preparations with deworming activity. The results showed that the ethyl acetate extract, butanol extract, and aqueous extract of C. sieberiana significantly inhibited larval migration of Haemonchus contortus, and the tannin-rich fractions also exhibited inhibitory effects. The chemical analysis identified 28 flavan-3-ols, including mono- and dimer forms, as well as newly described trimers.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Dimitrios Evangelos Miliordos, Anastasios Alatzas, Nikolaos Kontoudakis, Angeliki Kouki, Marianne Unlubayir, Marin-Pierre Gemin, Alexandros Tako, Polydefkis Hatzopoulos, Arnaud Lanoue, Yorgos Kotseridis
Summary: This study investigated the impact of two biostimulants, abscisic acid and chitosan, on the polyphenol metabolism of the Greek grapevine cultivar Savvatiano. The results showed that both abscisic acid and chitosan treatments increased anthocyanins during veraison, and resulted in higher amounts of stilbenoids at harvest. The gene expression related to stilbenoids and anthocyanin biosynthesis was induced by the applications of abscisic acid and chitosan, leading to increased accumulation of these compounds. However, the changes in other phenolic compound concentrations and gene expression profiles were mostly observed in the first vintage.
Article
Evolutionary Biology
Clement Cuello, Emily Amor Stander, Hans J. Jansen, Thomas Duge de Bernonville, Arnaud Lanoue, Nathalie Giglioli-Guivarc'h, Nicolas Papon, Ron P. Dirks, Michael Krogh Jensen, Sarah Ellen O'Connor, Sebastien Besseau, Vincent Courdavault
Summary: This study reports the first genome assembly and annotation of a Voacanga species, which will contribute significantly to future comparative and evolutionary studies in MIA-producing plants.
GENOME BIOLOGY AND EVOLUTION
(2022)
Article
Food Science & Technology
Ines L. Cabral, Antonio Teixeira, Manon Ferrier, Arnaud Lanoue, Joana Valente, Frank S. Rogerson, Fernando Alves, Susana M. P. Carvalho, Hernani Geros, Jorge Queiroz
Summary: Climate changes are causing grapes to mature faster in many regions, but higher temperatures can lead to unbalanced wines. A study tested the use of crop forcing in delaying the maturation of 'Touriga Nacional' vines in the Douro Region. Results showed that while crop forcing delayed ripening, it also severely impacted grape production. However, it did result in berries with lower pH, higher acidity, and increased phenolic content, potentially improving wine quality.
Article
Plant Sciences
Leo Bellenger, Eric Ducos, Muriel Feinard-Duranceau, Cecile Vincent-Barbaroux, Arnaud Lanoue, Marianne Unlubayir, Cecile Abdallah, Nathalie Pourtau, Benoit Porcheron, Anne Marmagne, Alexandra Launay-Avon, Christine Paysant Le Roux, Louis-Valentin Meteignier, Olivier Pichon, Christelle Dutilleul
Summary: This study aims to investigate the role of protein farnesylation in plant responses to nitrogen by studying the metabolic and phenotypic adaptations of the era1.8 mutant to different NH4NO3 supplies. The results show that era1.8 exhibits exacerbated responses to nitrogen starvation, with increased root/shoot ratio, anthocyanins content, and carbon/nitrogen ratio. Transcriptomic analysis reveals the over-expression of genes related to nitrogen starvation adaptation and identifies two genes encoding uncharacterized farnesylated proteins. Overall, the study highlights the involvement of protein farnesylation in plant adaptation to nitrogen nutritional stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Kevin Billet, Sebastien Salvador-Blanes, Thomas Duge De Bernonville, Guillaume Delanoue, Florent Hinschberger, Audrey Oudin, Vincent Courdavault, Olivier Pichon, Sebastien Besseau, Samuel Leturcq, Nathalie Giglioli-Guivarc'h, Arnaud Lanoue
Summary: Using spatial metabolomics and correlation-based networks, this study investigated how continuous changes in soil features and topography may impact the polyphenol composition in grape canes. The results showed correlations between soil and topographic variables and metabolomic responses. Spatial metabolomics driven by correlation-based networks can be used as a new field-phenotyping tool in precision agriculture.
Article
Plant Sciences
Dimitrios-Evangelos Miliordos, Anastasios Alatzas, Nikolaos Kontoudakis, Marianne Unlubayir, Polydefkis Hatzopoulos, Arnaud Lanoue, Yorgos Kotseridis
Summary: Grape berries are a significant source of phenolic compounds, and a practice using biostimulants has been developed to enhance phenolic content. A field experiment was conducted to investigate the effect of benzothiadiazole on polyphenol biosynthesis during grape ripening. The results showed the induction of genes involved in anthocyanins and stilbenoids biosynthesis, leading to increased phenolic compounds in wines from treated grapes.
Article
Plant Sciences
Henrique Noronha, Angelica Silva, Virginie Garcia, Kevin Billet, Alberto C. P. Dias, Arnaud Lanoue, Philippe Gallusci, Hernani Geros
Summary: Stilbenoids, important phenylpropanoid compounds synthesized in grapevine in response to stress, have been mainly described in berries. However, this study shows that they can also accumulate in the woody canes during bud burst, and their accumulation is correlated with the increased expression of stilbene synthase genes and the transcription factor VviMYB14. Additionally, there is a general stimulation of the plastidial shikimate pathway.
Article
Biochemistry & Molecular Biology
Armando Moreno-Velasco, Mabel Fragoso-Serrano, Pedro de Jesus Flores-Tafoya, Sebastian Carrillo-Rojas, Elihu Bautista, Suzana Guimara Leitao, Jhon F. Castaneda-Gomez, Rogelio Pereda-Miranda
Summary: This study investigated the roots of Operculina hamiltonii, a traditional medicinal plant in Brazil. Three new acyl sugars or resin glycosides were identified and their structures elucidated. The combination of these compounds with other drugs showed enhanced cytotoxic effects on breast cancer cells.
Article
Biochemistry & Molecular Biology
Qian-Qing Liu, Lin-An Xiong, Jia-Yu Qian, Ting-Ting Gong, Lie-Feng Ma, Luo Fang, Zha-Jun Zhan
Summary: A new naturally occurring compound, Linderagatins C-F (1-4), with the structures of diaryltetrahydrofuran-type 7,9'-dinorlignans, was discovered in the roots of Lindera aggregata. The neuroprotective effects of compounds 2 and 3 on erastin-induced ferroptosis in HT-22 cells were found to be significant.
