Article
Forestry
Marilia Shibata, Cileide M. M. Coelho, Julio A. de Garighan, Henrique P. dos Santos, Cristhyane G. Araldi, Marcelo Maraschin
Summary: Plant hormones play a crucial role in processes such as reserve accumulation, cellular activity, and physiological responses during seed development. Environmental conditions during development affect the time when seeds reach physiological maturity and hormone levels. The antagonistic relationship between abscisic acid and auxins is highlighted as a regulatory factor for germination.
Article
Plant Sciences
Yang Liu, Yu Zhang, Yi Zheng, Xinghua Nie, Yafeng Wang, Wenjie Yu, Shuchai Su, Qingqin Cao, Ling Qin, Yu Xing
Summary: Research has found that cultivated Chinese chestnut seeds have a higher germination rate due to structural variations in the genome, downregulation of ABA synthesis genes, and upregulation of beta-amylase synthesis genes. Additionally, phosphatidic acid synthesis genes are highly expressed during the seed germination stage of wild Chinese chestnut and may play a role in recalcitrant seed germination. These findings provide new insight into the regulation of wild seed germination and promote natural regeneration and succession in forest ecosystems.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Forestry
Newton Clovis Freitas da Costa, Lilian Iara Bet Stedille, Miguel Busarello Lauterjung, Tiago Montagna, Rafael Candido-Ribeiro, Alison Paulo Bernardi, Adelar Mantovani, Mauricio Sedrez dos Reis, Rubens Onofre Nodari
Summary: This study examines the genetic diversity, structure, and mating system of Araucaria angustifolia within populations, generations, and years. Results show that genetic divergences are mainly between populations, followed by differences among years and then among generations. Recommendations for seed collection may vary over the years, and multiyear analyses provide a comprehensive understanding of genetic aspects for long-lived tree species.
FOREST ECOLOGY AND MANAGEMENT
(2021)
Article
Plant Sciences
Monica Escandon, Ezequiel D. Bigatton, Victor M. Guerrero-Sanchez, Tamara Hernandez-Lao, Maria-Dolores Rey, Jesus V. Jorrin-Novo, Maria Angeles Castillejo
Summary: Proteases and protease inhibitors have been identified in the recalcitrant species Quercus ilex using in silico and wet methods, with a focus on their presence during seed germination. The study found that the Q. ilex transcriptome database contained a large number of proteases and protease inhibitors, with serine and metallo types being the most represented. Proteomics and activity assays revealed changes in proteases and protease inhibitors during germination, with different patterns observed in cotyledon and embryo axis tissues. The results highlight the importance of protein degradation and mobilization during the germination process in Quercus ilex seeds.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Danielle Specht Malta, Gabriel Goetten de Lima, Matheus Samponi Tucunduva Arantes, Andre Eduardo Biscaia de Lacerda, Alvaro Luiz Mathias, Washington Luiz Esteves Magalhaes, Cristiane Vieira Helm, Maria Lucia Masson
Summary: The effect of harvest location on cooked pinhao seeds (Araucaria angustifolia) was investigated, and significant differences between different locations were found. Geographic location was identified as one of the most important factors. Cooked pinhao seeds are a good source of nutrients and can contribute to a healthy diet. Additionally, the commercial use of pinhao seeds can help protect the endangered Araucaria angustifolia and improve the economy of households and small producers.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Forestry
Higor Fernando Salvador, Hellen Cassia Mazzottini-dos-Santos, Daiane Souza Dias, Alcinei Mistico Azevedo, Paulo Sergio Nascimento Lopes, Yule Roberta Ferreira Nunes, Leonardo Monteiro Ribeiro
Summary: Mauritia flexuosa forms persistent soil seed banks in humid environments, contributing to its reproductive success and the recovery of degraded areas. The permanence of seeds in the banks is not affected by soil attributes but is influenced by predator and pathogen attacks. Seed viability is not affected by permanence in the banks, as the seeds have strong homeostatic capacities. Dormancy levels decrease in the banks, promoting germination.
FOREST ECOLOGY AND MANAGEMENT
(2022)
Article
Forestry
Daniela Goeten, Francine L. Farias-Soares, Gladys D. Rogge-Renner, Maria L. T. Pereira, Christina Walters, Vanildo Silveira, Claudete S. Catarina, Miguel P. Guerra, Neusa Steiner
Summary: The study examined the biochemical changes in Araucaria angustifolia seeds during different stages of development. It was found that the ability to tolerate dehydration and cryopreservation is related to the transition from the early-to-late embryo stage. The study also observed variations in carbohydrate and protein content as well as the presence of dehydrin-like proteins at different stages of seed development.
TREES-STRUCTURE AND FUNCTION
(2023)
Article
Plant Sciences
Shifeng Li, Ning Yan, Mohsin Tanveer, Zhenyong Zhao, Li Jiang, Hongling Wang
Summary: This study investigated the response of seed germination of Peganum harmala, a valuable medicinal plant, to different environmental stresses in arid, saline regions. The findings showed that photoperiod and temperature significantly influenced germination, with the optimal temperature range observed at 30/15°C. The type and concentration of salinity also had a significant effect on germination and seedling growth. This study provides valuable information on the germination ecology of P. harmala, which can be useful for its efficient propagation and utilization.
Article
Biochemistry & Molecular Biology
Madiha Zaynab, Dezhuo Pan, Mahpara Fatima, Yasir Sharif, Shipin Chen, Wei Chen
Summary: The low germination rate of Cyclobalanopsis gilva seeds is attributed to insufficient energy supply, with proteins related to carbohydrate metabolism up-accumulated in germinated seeds and no changes or down-accumulation in non-germinated seeds.
Review
Plant Sciences
Julia Mergner, Bernhard Kuster
Summary: Proteins play essential roles in cellular processes, and studying protein expression, interactions, and modifications is crucial for understanding the molecular mechanisms underlying plant phenotypes. Mass spectrometry technology allows for the global identification and quantification of thousands of proteins, revealing dynamic changes in the plant proteome.
ANNUAL REVIEW OF PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anielle de Oliveira, Thaysa Fernandes Moya Moreira, Ana Luisa Silva Pepinelli, Luis Gustavo Medice Arabel Costa, Luana Eloisa Leal, Tamires Barlati Vieira da Silva, Odinei Hess Goncalves, Rafael Porto Ineu, Maria Ines Dias, Lillian Barros, Rui M. V. Abreu, Isabel C. F. R. Ferreira, Livia Bracht, Fernanda Vitoria Leimann
Summary: The study on extracts from pinhao bio-residues revealed that the PS extract has better antioxidant and alpha-amylase inhibition properties, while the CW extract is the most effective cholinesterase inhibitor. In addition, the PS extract showed cytotoxicity against various cancer cell lines.
Review
Biochemistry & Molecular Biology
Indrani Bera, Michael O'Sullivan, Darragh Flynn, Denis C. Shields
Summary: Legume seed protein is less digestible than animal protein, possibly due to defenses against herbivores. Germination increases proteolysis and digestibility by reducing anti-nutrient protease inhibitors, activating proteases, and breaking down storage proteins. Further research is needed to understand the mechanisms behind improved digestibility and guide the development of more digestible food preparations.
Article
Forestry
Rafael T. Resende, Pedro Italo T. Silva, Orzenil B. Silva-Junior, Miguel L. Menezes Freitas, Alexandre M. Sebbenn, Valderes A. Sousa, Ananda de Aguiar, Dario Grattapaglia
Summary: Genetic variation within and between provenances of Araucaria angustifolia was investigated, revealing a trend of increasing heritabilities over time within most provenances. The study found substantial genetic variation within families, suggesting the potential for efficient early selection. Growth curves showed that trees primarily invest in height before shifting to diameter growth, with considerable variation across provenances for the optimal age and tree volume at which growth peaks. The data also indicated the potential for accurate early selection for growth at age 7-10, with the possibility of shortening harvest age by selecting the best individuals and families. These results highlight the potential for expanding investments in breeding and plantation forestry of A. angustifolia, which could contribute to enhancing conservation efforts for this iconic subtropical conifer.
FOREST ECOLOGY AND MANAGEMENT
(2021)
Article
Ecology
Keyvan Maleki, Carol C. Baskin, Jerry M. Baskin, Mohadeseh Kiani, Iraj Alahdadi, Elias Soltani
Summary: Germination timing is crucial for plant survival and niche breadth. A modeling approach was used to compare the germination thermal niche of nine populations of N. sativa. Results showed that afterripening increased germination percentage, synchrony, and thermal niche breadth.
ECOLOGY AND EVOLUTION
(2022)
Article
Biochemical Research Methods
Sarah K. Osama, Edward D. Kerr, Adel M. Yousif, Toan K. Phung, Alison M. Kelly, Glen P. Fox, Benjamin L. Schulz
Summary: Germination is a critical process for flowering plant reproduction and industrial malting, while seed dormancy can lead to inefficiencies in malt production. Proteomic analysis identified significant changes during germination, including a rapid decrease in proteins involved in desiccation tolerance and germination inhibition, as well as reductions in lipid, protein, and nutrient reservoir storage. These consistent changes between laboratory and industrial scales demonstrate the utility of laboratory-scale germination as a model system for industrial malt house processes.
JOURNAL OF PROTEOMICS
(2021)
Article
Plant Sciences
Evandro Alexandre Fortini, Diego Silva Batista, Sergio Heitor Sousa Felipe, Tatiane Dulcineia Silva, Ludmila Nayara Freitas Correia, Leticia Monteiro Farias, Daniele Vidal Faria, Vitor Batista Pinto, Claudete Santa-Catarina, Vanildo Silveira, Clelia De-la-Pena, Eduardo Castillo-Castro, Wagner Campos Otoni
Summary: This study investigated the proteomic and epigenetic changes involved in the morphophysiological responses of Pfaffia glomerata to salt stress and the demethylating agent 5-azacytidine. The results revealed differentially accumulated proteins related to amino acid and carbohydrate metabolism, and specialized metabolism, as well as global DNA methylation levels and 20-E biosynthesis. The findings suggest the potential regulatory proteins and epigenetic changes that contribute to salt stress tolerance and 20-E biosynthesis in P. glomerata.
Article
Biochemical Research Methods
Luiza Giacomolli Polesi, Hugo Pacheco de Freitas Fraga, Felipe Astolpho Almeida, Vanildo Silveira, Miguel Pedro Guerra
Summary: This study identified proteins as markers of the embryogenic response in Guadua chacoensis and characterized the redox state of embryogenic cultures. The results showed that various proteins, including heat-shock proteins and enzymes involved in stress response mechanisms, were up- or down-regulated in embryogenic cultures compared to non-embryogenic cultures. Proteins associated with protein synthesis, energy sources, cell wall-related processes, and signaling transduction were also identified.
JOURNAL OF PROTEOMICS
(2023)
Article
Biotechnology & Applied Microbiology
Victor Paulo Mesquita Aragao, Kariane Rodrigues de Sousa, Tadeu dos Reis de Oliveira, Leandro Francisco de Oliveira, Eny Iochevet Segal Floh, Vanildo Silveira, Claudete Santa-Catarina
Summary: Polyamines and proteins play essential roles in the in vitro shoot development of Cedrela fissilis. Treatment with 6-benzyladenine and putrescine can promote shoot growth and affect the activities of Put biosynthesis enzymes and protein expression.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Luiz Eduardo Souza da Silva Irineu, Cleiton de Paula Soares, Tatiane Sanches Soares, Felipe Astolpho de Almeida, Fabricio Almeida-Silva, Rajesh Kumar Gazara, Carlos Henrique Salvino Gadelha Meneses, Luciano Pasqualoto Canellas, Vanildo Silveira, Thiago Motta Venancio, Fabio Lopes Olivares
Summary: The endophytic bacterium Herbaspirillum seropedicae can promote early growth in maize by increasing nitrogen uptake and assimilation, hormonal regulation, and enhancing carbon-nitrogen metabolism.
Article
Plant Sciences
Rosembrando Sosthenes Leite Carvalho, Tecla dos Santos Silva, Yrexam Rodrigues de Souza Ribeiro, Claudete Santa-Catarina, Jose Raniere Ferreira de Santana
Summary: This study aimed to induce callus in Cenostigma pyramidale and determine its embryogenic potential through histo-morphological and biochemical analysis. It was found that callus induction in Cenostigma pyramidale is possible using a culture medium containing 5.0 mu M BAP, 7.0 mu M 2,4-D, and 0.684 mM glutamine. Although cells with meristematic characteristics were present, somatic embryo differentiation was not observed. The growth curve of the callus showed distinct phases and the involvement of carbohydrates in callus growth was observed.
Article
Biochemistry & Molecular Biology
Daewon Kim, Dongqin Chen, Nagib Ahsan, Gabriel Lemes Jorge, Jay J. Thelen, Gary Stacey
Summary: Activation of the purinergic signaling pathway by phosphorylation of ILK5 leads to phosphorylation of MKK5 and MPK3/6, resulting in the induction of the plant innate immune response. The mechanism by which P2K1 activates the MAPK cascade is unclear.
Article
Biotechnology & Applied Microbiology
Renan Carrari-Santos, Rosana Gobbi Vettorazzi, Vitor Batista Pinto, Edinaldo de Oliveira Alves Sena, Jurandi Goncalves de Oliveira, Eliemar Campostrini, Vanildo Silveira, Claudete Santa-Catarina
Summary: This study evaluated the effects of sealing lids and culture media on in vitro seedling growth of Dalbergia nigra, with a focus on ethylene accumulation and changes in the protein profile. The results showed that sealing lids with microporous membranes promoted better seed germination and improved seedling development. The absence of microporous membranes in the culture media led to increased CO2 levels and leaf abscission. Furthermore, the accumulation of ethylene and proteins was influenced by both the culture media and the presence of microporous membranes.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Microbiology
Lumi Shiose, Marcia S. Vidal, Angelo S. S. Heringer, Luciano S. S. Vespoli, Vanildo Silveira, Jose Ivo Baldani, Ederson da Conceicao Jesus
Summary: This study examined the proteome of two common bean genotypes to investigate the metabolic shifts in leaf metabolism under inoculation and mineral nitrogen supply. The results showed that BRS Radiante had multiple affected pathways during symbiosis, while Ouro Negro had less affected metabolism.
Article
Plant Sciences
Benjamim Valentim da Silva, Tadeu dos Reis de Oliveira, Victor Paulo Mesquita Aragao, Almy Junior Cordeiro de Carvalho, Marta Simone Mendonca Freitas, Vanildo Silveira, Alessandro Coutinho Ramos, Claudete Santa-Catarina
Summary: This study evaluated the effects of different concentrations of iron(II) sulfate and pH on germination, growth, and nutrient and polyamine contents in Cedrela fissilis seedlings. The results showed that iron(II) sulfate and pH treatments decreased germination and fresh matter, and higher concentrations of iron(II) sulfate at pH 3 increased the content of Fe, Mn, Zn, Ca, and N in the roots. An increase in polyamine content in the roots may be an adaptive response to iron stress. The findings suggest that C. fissilis has the potential for ecological restoration in iron-contaminated areas.
BRAZILIAN JOURNAL OF BOTANY
(2023)
Article
Plant Sciences
Kleiton Lima de Godoy Machado, Daniele Vidal Faria, Marcos Bruno Silva Duarte, Lazara Aline Simoes Silva, Tadeu dos Reis de Oliveira, Thais Castilho Arruda Falcao, Diego Silva Batista, Marcio Gilberto Cardoso Costa, Claudete Santa-Catarina, Vanildo Silveira, Elisson Romanel, Wagner Campos Otoni, Fabio Tebaldi Silveira Nogueira
Summary: Age-dependent biosynthesis of the apocarotenoid bixin in the achiote tree (Bixa orellana L.) is modulated by miRNA156 (miR156) and its main targets, BoSPLs. Overexpression of miR156 resulted in reduced bixin production and increased abscisic acid levels in annatto plants. The expression modulation of key genes in carotenoid biosynthesis was associated with diverting the carbon flux from bixin to abscisic acid. The low accumulation of most secondary metabolite-related enzymes in miR156ox leaves suggests that miR156-targeted BoSPLs may be required to activate multiple secondary metabolic pathways.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Armando Moreno-Velasco, Mabel Fragoso-Serrano, Pedro de Jesus Flores-Tafoya, Sebastian Carrillo-Rojas, Elihu Bautista, Suzana Guimara Leitao, Jhon F. Castaneda-Gomez, Rogelio Pereda-Miranda
Summary: This study investigated the roots of Operculina hamiltonii, a traditional medicinal plant in Brazil. Three new acyl sugars or resin glycosides were identified and their structures elucidated. The combination of these compounds with other drugs showed enhanced cytotoxic effects on breast cancer cells.
Article
Biochemistry & Molecular Biology
Qian-Qing Liu, Lin-An Xiong, Jia-Yu Qian, Ting-Ting Gong, Lie-Feng Ma, Luo Fang, Zha-Jun Zhan
Summary: A new naturally occurring compound, Linderagatins C-F (1-4), with the structures of diaryltetrahydrofuran-type 7,9'-dinorlignans, was discovered in the roots of Lindera aggregata. The neuroprotective effects of compounds 2 and 3 on erastin-induced ferroptosis in HT-22 cells were found to be significant.
