4.7 Article

Differential expression of proteins induced by lead in the Dwarf Sunflower Helianthus annuus

Journal

PHYTOCHEMISTRY
Volume 71, Issue 13, Pages 1460-1465

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2010.05.018

Keywords

Dwarf Sunflower; Helianthus annuus; Compositae; Hyperaccumulation; Tandem mass spectrometry (MS/MS); Lead; 2-DE

Funding

  1. Ohio Plant Biotechnology Consortium through the Ohio State University, Ohio Agricultural Research and Development Center
  2. Cleveland State University Research Council

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The Dwarf Sunflower (Helianthus annuus) is a hyperaccumulator of toxic metals including cadmium (Cd), mercury (Hg), nickel (Ni), and lead (Pb). In order to identify stress response to Pb, plants were exposed to a mixture of 30 mg/l of three ions, Cd, Cr, and Ni, with and without Pb. Soluble proteins were resolved by two-dimensional gel electrophoresis. Four proteins were differentially expressed and very abundant in the leaf samples after plants were exposed to all these four metals. The first protein spot contained two proteins: chitinase and a chloroplast drought-induced stress protein CDSP-34. The second spot contained a thaumatin-like protein. Two proteins in spot 3 were identified as heat-shock cognate 70-1 and the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Several peptides were identified in spot 4 but none could be matched to any sequence in the NCBI database. (C) 2010 Elsevier Ltd. All rights reserved.

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