Article
Cell Biology
Enrica Pellegrino, Beren Aylan, Claudio Bussi, Antony Fearns, Elliott M. Bernard, Natalia Athanasiadi, Pierre Santucci, Laure Botella, Maximiliano G. Gutierrez
Summary: Peroxisomes play a role in restricting cytosolic Mycobacterium tuberculosis (Mtb) infection in human macrophages by increasing ROS levels.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Plant Sciences
Mengjiao Ruan, Wenliang He, Hao Sun, Chaiyan Cui, Xiangxiang Wang, Ruiling Li, Xiaomin Wang, Yurong Bi
Summary: This study investigates the role of cytosolic glucose-6-phosphate dehydrogenases (Cyt-G6PD) in embryo development of Arabidopsis. The dysfunction of Cyt-G6PD delays seed germination, inhibits seedling growth, decreases plant height and silique length, and increases abortion rate of siliques and seeds. Moreover, the dysfunction of Cyt-G6PD affects the accumulation of fatty acids and storage proteins. Exogenous application of fatty acids can alleviate the delayed germination of g6pd5/6 seeds. RT-qPCR results confirm the regulatory role of Cyt-G6PD in transcription of early embryo development genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Review
Genetics & Heredity
Md. Sifatul Islam, Ajit Ghosh
Summary: Aldehyde dehydrogenases (ALDHs) play an important role in plants by serving as scavengers for reactive aldehydes and responding to stress. Both animals and plants have multiple ALDH families, with plants having 14 families. The expansion of ALDH isoforms in plants allows for diverse functions. Studies have shown that ALDH is crucial for plant development and environmental stress adaptability.
Article
Microbiology
Natsuhei Suzuki, Daito Suda, Nguyen Thi Thuy Ngan, Namiko Gibu, Nguyen Lan Huong, To Kim Anh, Daisuke Kasai
Summary: The gram-positive rubber-degrading bacterium Nocardia farcinica NBRC 15,532 can utilize poly(cis-1,4-isoprene) as a carbon and energy source and produce metabolites. Two ALDH genes (NF2_RS14000 and NF2_RS14385) may be involved in the degradation of oligo-isoprene aldehydes.
Article
Biochemistry & Molecular Biology
Superior Syngkli, Bidyadhar Das
Summary: This study characterized the properties and functions of human mGPDH and cGPDH enzymes. By optimizing the genes, overexpressing, and purifying these enzymes, it was found that mGPDH is a monomeric enzyme with optimal activity in Tris-HCl buffer (pH 7.4), while cGPDH is a homodimer with optimal activity in imidazole buffer (pH 8.0). These methods help understand the role of these enzymes in metabolic disorders.
Article
Chemistry, Multidisciplinary
Gonzalo de Gonzalo, Caroline E. Paul
Summary: This review discusses recent advances in the application of alcohol dehydrogenases in cascade reactions, collaborating with other enzyme classes, chemocatalysts, or organocatalysts to obtain high value-added products such as chiral diols and amino alcohols. The review also looks towards future developments in chemoenzymatic and organoenzymatic cascades.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2021)
Review
Oncology
Jakob Pueschel, Anna Dubrovska, Ielizaveta Gorodetska
Summary: Cancer stem cells are the engine of tumor progression and a source of tumor regrowth after treatment. Targeting CSCs might be a powerful tool to overcome tumor resistance and increase the efficiency of current cancer treatment strategies, with high ALDH activity being a common marker of prostate CSCs.
Article
Biochemistry & Molecular Biology
Muhammad Imran, Muhammad Zeeshan Munir, Sara Ialhi, Farhat Abbas, Muhammad Younus, Sajjad Ahmad, Muhmmad Kashif Naeem, Muhammad Waseem, Arshad Iqbal, Sanober Gul, Emilie Widemann, Sarfraz Shafiq
Summary: This study identified and classified 12 MDH genes in tomato, and analyzed their expression patterns and functional characteristics. The results showed that tomato MDH genes were differentially expressed in different tissues and fruit development stages, and exhibited specific substrate and co-factor specificity in the conversion process of malate to oxaloacetate. Additionally, the study revealed the involvement of tomato MDH genes in salt stress tolerance through the co-localization analysis with QTLs. These findings provide a foundation for further functional analysis and genetic improvement in tomatoes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Jianling Song, Xiaoyan Zou, Pandao Liu, Juan Andres Cardoso, Rainer Schultze-Kraft, Guodao Liu, Lijuan Luo, Zhijian Chen
Summary: The importance of malate dehydrogenase (MDH) in cellular metabolism, redox homeostasis, and response to nutrient and metal stresses in Stylosanthes guianensis (stylo) is demonstrated in this study.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Lisa Hitschler, Laura Sofie Nissen, Michelle Kuntz, Mirko Basen
Summary: By studying enzymes in Thermoanaerobacter, it was found that AdhE and AdhB play important roles in alcohol production from sugar and reduction of organic acids, while the functions of AdhA-type enzymes are less clear. Future research is needed to elucidate the role of aldehyde:ferredoxin oxidoreductase in bio-alcohol formation.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biology
Marcia R. Couto, Joana L. Rodrigues, Ligia R. Rodrigues
Summary: This study characterized novel enzymes with potential for UDP-GlcA biotechnological production, showing that the enzymes were active in both prokaryotic and eukaryotic hosts. Through expression in different hosts, in vivo and in vitro production of UDP-GlcA was achieved. These findings provide valuable alternatives for future industrial applications.
Article
Plant Sciences
Xiaojing Lin, Linxuan Huang, Huilin Liang, Chen Hou, Xuli Ling, Yuanxia Chen, Peng Yang, Qingwen Wu, Haiying Zhao, Sirong Wu, Ruoting Zhan, Dongming Ma, Jinfen Yang
Summary: Genome-wide screening reveals functional diversification of borneol dehydrogenase (BDH) in Wurfbainia villosa. Understanding the functional diversity and biosynthesis mechanism of BDHs are of great importance.
Article
Biochemistry & Molecular Biology
Przemyslaw Mielczarek, Tymoteusz Slowik, Jolanta Helena Kotlinska, Piotr Suder, Anna Bodzon-Kulakowska
Summary: This study investigated the derivatization of cholesterol with betaine aldehyde in tissue slices and evaluated the impact of different sample preparation methods on the signal from the derivatization product. The results demonstrated that the proposed approach could be utilized for cholesterol analysis in various tissues.
Article
Food Science & Technology
Eliana Castanares, Lucia da Cruz Cabral, Maria Dinolfo, Birgitte Andersen, Sebastian A. Stenglein, Andrea Patriarca
Summary: In this study, the Alternaria population infecting different cultivars of barley grains in Argentina was characterized using a polyphasic approach. The results showed that Alternaria had a higher incidence than Fusarium in all barley samples, with A. tenuissima and A. infectoria being the predominant species-groups. The study also found correlations between Alternaria frequency and field temperature, as well as differences in metabolite profiles between sections Alternaria and Infectoriae.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Food Science & Technology
Johnatt Allan Rocha de Oliveira, Paula de Paula Menezes Barbosa, Gabriela Alves Macedo
Summary: This paper evaluated the methodologies for extracting phenolic compounds using Deep Eutectic Solvents (DES) in combination with pectinlyase. The results showed that DES associated with pectinlyase had higher concentration of phenolic compounds and antioxidant activity, indicating its excellent potential for extracting flavonoids from citrus pomace.
Article
Biochemistry & Molecular Biology
Ruiqin Yi, Hirofumi Narimoto, Miku Nozoe, Tsuyoshi Ichiyanagi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Naoki Ube, Miho Nishizaka, Tsuyoshi Ichiyanagi, Kotomi Ueno, Shin Taketa, Atsushi Ishihara
Article
Biochemistry & Molecular Biology
Jiro Arima, Shota Tokai, Masanori Chiba, Tsuyoshi Ichiyanagi, Yukinori Yabuta, Nobuhiro Mori, Tadanori Aimi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2014)
Article
Microbiology
Totsaporn Srisapoomi, Tsuyoshi Ichiyanagi, Hiromitsu Nakajima, Tadanori Aimi, Sophon Boonlue
CURRENT MICROBIOLOGY
(2015)
Article
Plant Sciences
Shigeki Nishino, Roxana Y. Parada, Tsuyoshi Ichiyanagi, Nitaro Maekawa, Norihiro Shimomura, Hiroshi Otani
JOURNAL OF PHYTOPATHOLOGY
(2013)
Article
Chemistry, Organic
Ruiqin Yi, Atsushi Ogaki, Mayumi Fukunaga, Hiromitsu Nakajima, Tsuyoshi Ichiyanagi
Article
Pharmacology & Pharmacy
Tomohiko Kayano, Naoki Kitamura, Shunsuke Miyazaki, Tsuyoshi Ichiyanagi, Norihiro Shimomura, Izumi Shibuya, Tadanori Aimi
Article
Biochemistry & Molecular Biology
Tsuyoshi Ichiyanagi
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY
(2014)
Article
Chemistry, Organic
Sujit Manmode, Moeko Kato, Tsuyoshi Ichiyanagi, Toshiki Nokami, Toshiyuki Itoh
ASIAN JOURNAL OF ORGANIC CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Tomohiro Bito, Yukinori Yabuta, Tsuyoshi Ichiyanagi, Tsuyoshi Kawano, Fumio Watanabe
Article
Entomology
Atsushi Ishihara, Chisaki Ashida, Naoki Ube, Masato Abe, Hidetaka Hiyoshi, Kazuto Umezu, Naoki Endo, Kozue Sotome, Nitaro Maekawa, Akira Nakagiri, Kumiko Osaki-Oka, Tsuyoshi Ichiyanagi, Kotomi Ueno
JOURNAL OF PESTICIDE SCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Tomohiro Bito, Kyohei Koseki, Ryota Asano, Nakaba Ueda, Takuhiro Yamada, Yukinori Yabuta, Tsuyoshi Ichiyanagi, Atsushi Ishihara, Kishi Watanabe, Fumio Watanabe
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2020)
Article
Chemistry, Organic
Tsuyoshi Ichiyanagi, Hirofumi Narimoto, Naoki Ohtani
Summary: The branched inner-core oligosaccharides of neisserial lipooligosaccharides were successfully synthesized in this study, indicating that glycosylation reaction in toluene proceeded smoothly. Additionally, the consecutive branched hexasaccharide obtained through fragment condensation was characterized by specific structural features.
Article
Biochemistry & Molecular Biology
Shogo Hamajima, Naoko Komura, Hide-Nori Tanaka, Akihiro Imamura, Hideharu Ishida, Tsuyoshi Ichiyanagi, Hiromune Ando
Summary: Chemical synthesis of Kdo-containing glycans is important for developing vaccines against pathogenic bacteria. The alpha-stereoselective glycosidation of Kdo has been achieved using a macrobicyclic donor. In this study, the protection of the 4-OH group and the use of a selectively removable booster allowed the synthesis of the dimeric Kdo sequence.
Article
Biotechnology & Applied Microbiology
Yusei Kobayashi, Yasunori Akagi, Kaori Tsubaki, Emiko Shimoda, Takashi Kikuchi, Naoki Endo, Tsuyoshi Ichiyanagi, Akira Nakagiri, Tadashi Nishida, Atsushi Ishihara
Summary: Pharmacological intervention of circadian rhythms is a potential approach for addressing health problems caused by disrupted circadian rhythms. A study found that a compound derived from the Cyclocybe erebia mushroom enhanced the oscillation of a luciferase gene and extended the period of bioluminescence. Further analysis revealed the structure of the compound, which was named cyclocircadins A and B. These findings suggest that natural diterpenoids could be a potential source for compounds affecting circadian rhythms.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)