Protection of pigs against pandemic swine origin HIM influenza A virus infection by hemagglutinin- or neuraminidase-expressing attenuated pseudorabies virus recombinants

Influenza is an important respiratory disease of pigs, and may lead to novel human pathogens like the 2009 pandemic HI NI swine-origin influenza virus (SoIV). Therefore, improved influenza vaccines for pigs are required. Recently, we demonstrated that single intranasal immunization with a hemagglutinin (HA)expressing pseudorabies virus recombinant of vaccine strain Bartha (PrV-Ba) protected pigs from H1N1 SoIV challenge (Klingbeil et al., 2014). Now we investigated enhancement of efficacy by prime-boost vaccination and/or intramuscular administration. Furthermore, a novel PrV-Ba recombinant expressing codon-optimized N1 neuraminidase (NA) was included. In vitro replication of this virus was only slightly affected compared to parental virus. Unlike HA, the abundantly expressed NA was efficiently incorporated into Pry particles. Immunization of pigs with the two Pry recombinants, either singly or in combination, induced B cell proliferation and the expected SoIV-specific antibodies, whose titers increased substantially after boost vaccination. After immunization of animals with either Pry recombinant H1N1 SoIV challenge virus replication was significantly reduced compared to PrV-Ba vaccinated or naive controls. Protective efficacy of HA-expressing PrV was higher than of NA-expressing PrV, and not significantly enhanced by combination. Despite higher serum antibody titers obtained after intramuscular immunization, transmission of challenge virus to naive contact animals was only prevented after intranasal prime-boost vaccination with HA-expressing PrV-Ba. (C) 2015 Elsevier B.V. All rights reserved.