Journal
VIROLOGY
Volume 484, Issue -, Pages 103-112Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.05.013
Keywords
AIDS; Apoptosis; DCIR; Dendritic cells; Extracellular vesicles; Exosomes; HIV-1; CD4 T lymphocytes; Neutrophils
Categories
Funding
- Canadian Institutes of Health Research (CIHR) HIV/AIDS initiative [MOP-188726, MOP-120235]
- Fonds de la Recherche en Sante du Quebec
- CIHR/HIV/AIDS initiative
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Exosomes are extracellular vesicles (EVs) that play a role in intercellular communication. Stimulation of dendritic cells by the HIV-1 virus triggers their release. HIV-1 binds to dendritic cells via dendritic cell immunoreceptor (DCIR). This study shows that inhibiting the binding to DCIR significantly decreases exosome release by HIV-1-pulsed dendritic cells. In addition, exosome release from Raji-CD4 expressing DCIR cells stimulated by anti-DCIR or HIV-1 is decreased when the immunoreceptor tyrosine-based inhibition motif (ITIM) signaling motif of DCIR is mutated. Unlike the EVs released from Raji-CD4-DCIR cells after antibody stimulation, those released from HIV-1-infected cells contain the pro-apoptotic protein DAP-3. Furthermore, EVs from HIV-1 pulsed dendritic cells increase spontaneous apoptosis in uninfected CD4 T lymphocytes while they decrease it in neutrophils. This study describes for the first time that DCIR plays a role in the release of exosomes strengthening the importance of this receptor and EVs/exosomes in HIV-1 pathogenesis. (C) 2015 The Authors. Published by Elsevier Inc.
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