Journal
PHYSICAL REVIEW LETTERS
Volume 101, Issue 24, Pages -Publisher
AMER PHYSICAL SOC
DOI: 10.1103/PhysRevLett.101.248101
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Funding
- Korea government (MOST) [R01-2007000-10854-0, R01-2007-00011674]
- Seoul RBD program
- Petroleum Research Fund ACS PRF [43923-AC7]
- National Research Foundation of Korea [R17-2007-017-01001-0, R01-2007-000-10854-0, R01-2007-000-11674-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Cisplatin was incidentally discovered to suppress cell division and became one of the most successful antitumor drugs. It is therapeutically active upon binding to DNA and locally kinking it. We demonstrate that after a bimodal modeling, the degree of platination of a single DNA molecule can be consistently and reliably estimated from elasticity measurements performed with magnetic tweezers. We predicted and measured for the first time two separate persistence lengths of kinked DNA at high and low tensions. We also directly observed that the degree of platination of DNA strongly depends on the concentration of sodium chloride as required for cisplatin's intracellular activity. Our study shows that micromanipulation techniques accurately reveal the degree of chemical modification of DNA which can be used for a new type of structure-sensitive biosensors.
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