Article
Fisheries
Qian Yang, Xiao-Dong Yang, Meng-Qun Liu, Chu Zeng, Hong-Kui Zhao, Kai-Wen Xiang, Zhi-Shuai Hou, Hai-Shen Wen, Ji-Fang Li
Summary: Rainbow trout, an important cold-water fish, is threatened by viruses and bacteria in the farming industry. Vibrio anguillarum, a common disease-causing pathogen, infects fish mainly through the skin, gills, lateral line, and intestine. In response to the infection, rainbow trout activate immunomodulatory genes and down-regulate tissue function-related genes, while a sustained inflammatory response can lead to tissue damage and death. These findings provide a theoretical basis for breeding rainbow trout with disease resistance.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Xiao-Dong Yang, Zhi-Shuai Hou, Meng-Qun Liu, Chu Zeng, Hong-Kui Zhao, Yuan-Ru Xin, Kai-Wen Xiang, Qian Yang, Hai-Shen Wen, Ji-Fang Li
Summary: This study identified 17 mkk genes in trout and showed functional diversity of different mkk subtypes. Bacterial infection resulted in up-regulation of certain mkk subtypes, suggesting their involvement in immune regulation through ERK, p38, and JNK pathways. Negative feedback mechanisms also played a role in the regulation of MAPK signaling pathways.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Vimbai Irene Machimbirike, Ignacio Vasquez, Trung Cao, Joy Chukwu-Osazuwa, Oluwatoyin Onireti, Cristopher Segovia, Pongsak Khunrae, Triwit Rattanarojpong, Marije Booman, Simon Jones, Manuel Soto-Davila, Brian Dixon, Javier Santander
Summary: This study investigates the genetic differences and evolution of the most common fish pathogen Vibrio anguillarum serotype O1. The O1 strain has two chromosomes and two typical plasmids. Furthermore, the O1 strain displays resistance to colistin sulphate, which differs from the O2 serotype and may be attributed to the ugd gene. The study also reveals that the genetic heterogeneity in the O-antigen biosynthesis gene cluster is characterized by the absence or presence of unique genes, which could result in differences in immune evasion mechanisms among serotypes.
Article
Immunology
Jongwon Lim, Suhee Hong
Summary: This study aimed to identify molecular mechanisms affected by a combined vaccine against Aeromonas salmonicida and Vibrio anguillarum. The vaccine upregulated gene expression associated with adaptive immune response markers and triggered differential expression of hundreds of genes on different days. Gene ontology and Kyoto Encyclopedia of Genetic and Genomic analyses identified immune system-related pathways and processes affected by the vaccine. Protein-protein interaction analysis revealed TNF as the central player in immune-related responses at different time points.
Article
Agriculture, Dairy & Animal Science
Stanislav Rimaso Kurpe, Irina Viktorovna Sukhovskaya, Ekaterina Vitalyevna Borvinskaya, Alexey Anatolievich Morozov, Aleksey Nikolaevich Parshukov, Irina Evgenyevna Malysheva, Alina Valeryevna Vasileva, Natalia Alexandrovna Chechkova, Tamara Yurevna Kuchko
Summary: This study investigated natural bacterial infection in fish farms and characterized the health status parameters of rainbow trout. Three subpopulations of infected trout with varying severity of damage, from severe lesions to asymptomatic fish, were identified. Unexpectedly, there was minimal metabolic difference between fish with moderate symptoms and fish with weak signs of pathology. The study also examined the pathogenesis of Vibrio anguillarum infection in rainbow trout and identified molecular markers for diagnosing health and immune status in trout.
Review
Endocrinology & Metabolism
Calum Urquhart, Ben Fleming, Ines Harper, Luigi Aloj, Ruth Armstrong, Liz Hook, Anna-May Long, Claire Jackson, Ferdia A. Gallagher, Mary A. McLean, Patrick Tarpey, Vasilis Kosmoliaptsis, James Nicholson, A. Emile J. Hendriks, Ruth T. Casey
Summary: There is increasing evidence supporting the use of temozolomide therapy for metastatic phaeochromocytoma/paraganglioma (PPGL) in adults with SDHx mutations. However, very little data is available for children. In this report, a 12-year-old female with a SDHB-related metastatic paraganglioma was treated with surgery followed by temozolomide therapy and showed a partial response.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Medicine, General & Internal
Iva Brcic, Alexandra Argyropoulos, Bernadette Liegl-Atzwanger
Summary: Gastrointestinal stromal tumors are the most common mesenchymal tumors of the gastrointestinal tract, with majority being sporadic and harboring mutations in KIT or PDGFRA genes. Wild-type GISTs lack these mutations, are clinically and molecularly heterogeneous, and can be divided into syndromic and non-syndromic subgroups. The use of SDHB by immunohistochemistry helps to further classify GIST into SDHB-retained and SDHB-deficient groups.
Article
Biochemistry & Molecular Biology
Jing Xing, Xiaoyu Jiang, Hongsen Xu, Xiuzhen Sheng, Xiaoqian Tang, Heng Chi, Wenbin Zhan
Summary: Immunization with pVAA induces effective local and systemic immune responses in flounders, significantly increasing the proportion of CD4(+) cells and upregulating genes involved in immune system activation. The specific antibodies against L. anguillarum were induced post-immunization, reaching a peak at 4-5 weeks, with a relative percent survival rate of 53.85 +/- 3.64% in the pVAA group, suggesting the potential of pVAA for effective protection against L. anguillarum.
MOLECULAR IMMUNOLOGY
(2021)
Article
Fisheries
Kaiyue Duan, Xin Tang, Jingzhuang Zhao, Guangming Ren, Yizhi Shao, Tongyan Lu, Baoquan He, Liming Xu
Summary: The study demonstrates that the vaccine prepared using genogroup HPNV isolate could stimulate nonspecific and specific immune responses, providing significant immune protection to vaccinated rainbow trout.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Amany M. Diab, Riad H. Khalil, Rasmia H. M. Abu Leila, Mahmoud M. Abotaleb, Mohamed A. Khallaf, Mahmoud A. O. Dawood
Summary: The study revealed that gills are the most common parasitic site for L. anguillarum and V. alginolyticus in cultured European sea bass. Intraperitoneal injection of V. alginolyticus FKC bacterin is the most effective administration route, providing protection against V. alginolyticus and cross-protection effects.
Article
Fisheries
He Yang, Ding Zhujin, Moonika H. Marana, Inger Dalsgaard, Jaafar Rzgar, Mathiessen Heidi, Karami M. Asma, Kania W. Per, Buchmann Kurt
Summary: Both autogenous and commercial ERM vaccines were effective in protecting rainbow trout from live Y. ruckeri bacteria. Fish receiving a high dosage of commercial vaccine showed increased levels of IgM expression post-challenge, indicating a correlation between vaccine dosage and protection.
JOURNAL OF FISH DISEASES
(2021)
Article
Immunology
Andrea Marsella, Francesco Pascoli, Tobia Pretto, Alessandra Buratin, Lorena Biasini, Miriam Abbadi, Luana Cortinovis, Paola Berto, Amedeo Manfrin, Marco Vanelli, Simona Perulli, Jesper S. Rasmussen, Dagoberto Sepulveda, Niccolo Vendramin, Niels Lorenzen, Anna Toffan
Summary: Despite the negative impact of VHS and IHN on European rainbow trout farming, no vaccines are commercially available in Europe. This study developed and tested two DNA vaccines encoding the glycoproteins of recent Italian VHSV and IHNV isolates. The results showed that the DNA vaccines were safe and efficient in reducing the impact of VHS and IHN in farmed rainbow trout.
Article
Fisheries
Valentina Wong-Benito, Felipe Barraza, Agustin Trujillo-Imarai, Daniela Ruiz-Higgs, Ruth Montero, Ana Maria Sandino, Tiehui Wang, Kevin Maisey, Christopher J. Secombes, Monica Imarai
Summary: In this study, the cytokine immune response against two proteins of infectious pancreatic necrosis virus (IPNV) in rainbow trout was examined. The results showed that VP1 induced cellular and humoral immune responses, while VP2-Flg resulted in a different cytokine expression profile. These findings provide new insights for the development of IPNV vaccines.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jongwon Lim, Yoonyoung Jang, Hyun-Ja Han, Suhee Hong
Summary: In this study, four strains of Vibrio anguillarum were isolated and found to have the same serotype, biochemical characteristics, and virulence factor genes. However, there were differences in haemolytic activity among these strains, with the less pathogenic strain displaying gamma-haemolytic activity and the virulent strains showing alpha-haemolytic activity and higher empA gene expression. The most virulent strain, V. anguillarum RTBHR, caused high mortality rates in rainbow trout and Coho salmon. A formalin-inactivated vaccine of V. anguillarum RTBHR successfully induced protective and specific immune responses in rainbow trout.
JOURNAL OF FISH DISEASES
(2023)
Article
Otorhinolaryngology
Leba Michael Sarkis, Rory Clifton-Bligh, David Veivers, Anthony James Gill
Summary: This study aimed to estimate the prevalence of succinate dehydrogenase (SDH) deficiency in patients with carotid body paragangliomas. Out of 64 identified cases, 43 (67%) were found to be SDH-deficient. These findings suggest that genetic testing and counseling should be offered to all patients with carotid body paragangliomas.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2023)
Article
Fisheries
Ilhan Aydin, Rafet Cagri Ozturk, Ercan Kucuk, Hamza Polat, Ilhan Altinok
Summary: This study compared the hatchery and growth performance of diploid and triploid turbot at 16 degrees C and 21 degrees C. Different water temperatures during rearing had significant effects on the weight and length of turbot, with triploids showing differences in growth performance.
Article
Fisheries
Mustafa Ture, Ilhan Altinok, Ayse Cebeci, Nihal Caliskan
AQUACULTURE RESEARCH
(2022)
Review
Fisheries
Adef Othan Kordon, Lesya Pinchuk, Attila Karsi
Summary: The immune system of jawed vertebrates consists of two major subsystems: the non-specific innate immune system and the specific adaptive immune system. The adaptive immune system in teleost fish allows for immunological memory and vaccination, making it crucial for disease control in aquaculture.
TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
(2022)
Article
Fisheries
Mohamed Sayed, Matt Griffin, Cynthia Ware, Ozan Ozdemir, Hasan C. Tekedar, Manal Essa, Attila Karsi, Mark L. Lawrence, Hossam Abdelhamed
Summary: This study characterizes the role of the BasS/BasR two-component system in Edwardsiella piscicida virulence and immune protection in catfish hosts. The mutants lacking basS and basR genes in E. piscicida were more sensitive to acidic environments and produced less biofilm. In vivo studies revealed significant attenuation and protection in vaccinated catfish. Further investigation is needed to determine the efficacy in hybrid catfish. These findings provide insights into the virulence mechanism of E. piscicida and suggest the potential of using Ep Delta basS and Ep Delta basR strains as vaccines.
JOURNAL OF FISH DISEASES
(2022)
Article
Agriculture, Multidisciplinary
I Yandi, R. C. Ozturk, Y. Terzi, S. Kayis, I Altinok
Summary: This study evaluated the effects of including black soldier fly prepupae in rainbow trout fingerling diets. The results showed that replacing 50% of fish meal with black soldier fly meal had no adverse effects on growth performance, liver characteristics, feed utilization, or fillet quality. Therefore, black soldier fly meal can safely be used in rainbow trout fingerling diets.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Review
Biodiversity Conservation
Myriam Heuertz, Silvia B. Carvalho, Juan Galindo, Baruch Rinkevich, Piotr Robakowski, Tsipe Aavik, Ilhan Altinok, Julia M. I. Barth, Helena Cotrim, Roos Goessen, Santiago C. Gonzalez-Martinez, Tine Grebenc, Sean Hoban, Alexander Kopatz, Barry J. McMahon, Ilga Porth, Joost A. M. Raeymaekers, Sabrina Traeger, Alejandro Valdecantos, Adriana Vella, Cristiano Vernesi, Pauline Garnier-Gere
Summary: Conserving biodiversity at genetic and community levels is crucial for the provision of ecosystem services. However, the application of genomics in biodiversity conservation and ecosystem service management is currently limited, primarily focusing on flagship species. To bridge this gap, a framework is proposed to guide genomics applications in management, including population supplementation, assisted migration, delimitation of conservation areas, and solving conservation trade-offs. Effective implementation of genomics-informed management actions requires collaboration between scientists and ecosystem service managers along with standardized workflows and training for managers.
BIOLOGICAL CONSERVATION
(2023)
Article
Fisheries
Rafet Cagri Ozturk, Ilhan Yandi, Yahya Terzi, Ilhan Altinok
Summary: This study investigated the effects of direct black soldier fly (BSF) feeding on the growth, health, and filet quality of rainbow trout. It was found that feeding dried BSF led to a reduction in growth, decreased feed intake, and constipation in the fish. The amino acid content of BSF prepupae was higher than that of commercial diet, and some fatty acid contents were similar to or higher than commercial diet. Feeding dried BSF once a day resulted in improvements in certain fatty acid levels in the fish.
TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
I. Yandi, R. C. Ozturk, M. Kocabas, I. Z. Kurtoglu, I. Altinok
Summary: This study investigated the effect of different organic waste substrates on the nutritional content of black soldier fly prepupae (BSFP). It found that BSFP reared on fruit and vegetable waste had lower protein and lipid content, but higher carbohydrate content compared to those reared on chicken waste meal and a mixture of the two. Additionally, BSFP reared on the mixture had higher levels of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids. These findings suggest that the use of certain organic waste substrates can enhance the nutritional value of BSFP and their potential as a sustainable protein source in aquafeeds.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Article
Fisheries
Sarker Mohammed Ibrahim Khalil, Chiara Bulfon, Marco Galeotti, Pier Luigi Acutis, Ilhan Altinok, Charalampos Kotzamanidis, Ana Isabel Vela, Lucio Fariano, Marino Prearo, Silvia Colussi, Donatella Volpatti
Summary: This study provides preliminary knowledge on the immunological relationship between rainbow trout and L. garvieae, suggesting that specific immune parameters could serve as reliable indicators of the fish's innate ability to fight infection.
JOURNAL OF FISH DISEASES
(2023)
Article
Microbiology
Nadia Stoppani, Silvia Colussi, Paolo Pastorino, Marino Prearo, Simona Sciuto, Ilhan Altinok, Rafet Cagri Ozturk, Mustafa Ture, Ana Isabel Vela, Maria del Mar Blanco, Charalampos Kotzamanidis, Konstantina Bitchava, Andigoni Malousi, Lucio Fariano, Donatella Volpatti, Pier Luigi Acutis, Jose Francisco Fernandez-Garayzabal
Summary: The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate L. garvieae from L. petauri for accurate discrimination between these two species. Seven SNPs were identified that separate L. garvieae from L. petauri based on the ITS region sequence. The 16S-23S rRNA ITS region can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak.
Article
Microbiology
Mohamed Sayed, Lakshmi Narayanan, Manal Essa, Mark Lawrence, Attila Karsi, Hossam Abdelhamed
Summary: Columnaris disease caused by Flavobacterium covae leads to significant economic losses in commercially important fish species worldwide. The US channel catfish industry is particularly vulnerable. Therefore, developing a vaccine to reduce the economic losses is urgently needed. This study aims to identify the main secreted extracellular products (SEPs) of F. covae and evaluate their potential for providing protection against columnaris disease in channel catfish.
Article
Microbiology
Eda Ozdemir, Hossam Abdelhamed, Ozan Ozdemir, Mark Lawrence, Attila Karsi
Summary: Virulent Aeromonas hydrophila (vAh) strains causing motile Aeromonas septicemia (MAS) in farmed channel catfish have posed a significant problem for over a decade. The pathogenicity of vAh in catfish is not well understood, which makes studying it crucial. In this study, a bioluminescent vAh (BvAh) was developed using a new bioluminescence expression plasmid and its interactions with catfish were visualized using bioluminescent imaging. The findings provide valuable insights into the pathogenicity of vAh in catfish and demonstrate potential entry portals for vAh.
Review
Fisheries
Raja Aadil Hussain Bhat, Ilhan Altinok
Summary: Antimicrobial resistance in aquaculture poses a significant threat to public health and the environment. Understanding the mechanisms driving AMR and the patterns of antibiotic usage is crucial for developing risk management strategies. Alternative approaches can be adopted to prevent and control fish diseases without negatively impacting ecosystems and public health.
TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
(2023)
Article
Microbiology
Ayse Cebeci, Mustafa Ture, Melike Alemdag, Ilhan Altinok
Summary: In this study, a bacteriophage specific to Aeromonas hydrophila was isolated from domestic wastewater. The phage was found to be icosahedral with a diameter of 60 nm. Based on the analysis of its genome sequence, the phage belongs to the genus Lahexavirus and does not contain antimicrobial resistance or virulence genes. Overall, the Lahexavirus APT65 phage may have potential as a therapeutic agent to tackle antimicrobial resistance in aquaculture.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2023)
Article
Fisheries
Ana Isabel Vela, Maria del Mar Blanco, Silvia Colussi, Charalampos Kotzamanidis, Marino Prearo, Ilhan Altinok, Pier Luigi Acutis, Donatella Volpatti, Patricia Alba, Fabiola Feltrin, Angela Ianzano, Lucas Dominguez, Jose Francisco Fernandez-Garayzabal
Summary: Lactococcosis is a globally prevalent infectious disease that has a significant economic and sanitary impact on the rainbow trout industry. This study reveals that both Lactococcus garvieae and Lactococcus petauri are implicated in the etiology of lactococcosis, and that L. petauri plays a significant role in the epidemiology of this disease. Additionally, some biochemical differences between the two species may serve as phenotypic markers for their presumptive recognition.
