Journal
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Volume 12, Issue 37, Pages 11498-11506Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c002737e
Keywords
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Funding
- European Union [FP7-228490]
- National Science Foundation [OTKA 47368, OTKA 68120, OTKA 68358]
- National Office for Research and Technology [NKFP 07 TB_INTER-HU, GVOP-3.2.1-2004-04-0099, -0005, -0352]
- Hungarian Academy of Sciences
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Tensiometry, sum-frequency vibrational spectroscopy, and atomic force microscopy were employed to assess the cell penetration ability of a peptide conjugate of the antituberculotic agent isoniazide. Isoniazide was conjugated to peptide (91)SEFAYGSFVRTVSLPV(106), a functional T-cell epitope of the immunodominant 16 kDa protein of Mycobacterium tuberculosis. As a simple but versatile model of the cell membrane a phospholipid Langmuir monolayer at the liquid/air interface was used. Changes induced in the structure of the phospholipid monolayer by injection of the peptide conjugate into the subphase were followed by tensiometry and sum-frequency vibrational spectroscopy. The drug penetrated lipid films were transferred to a solid support by the Langmuir-Blodgett technique, and their structures were characterized by atomic force microscopy. Peptide conjugation was found to strongly enhance the cell penetration ability of isoniazide.
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