Review
Biotechnology & Applied Microbiology
Xueliang Li, Shibin Liu, Jie Tan, Chunsheng Wu
Summary: The light-addressable potentiometric sensor (LAPS) is an electrochemical sensor based on the field-effect principle of semiconductors. It has the unique ability to address specific areas of the sensor using movable light, enabling two-dimensional image distribution detection of chemical/biomass. This review summarizes the advantages of LAPS as a sensing unit in microelectrochemical analysis systems and discusses the recent advances in LAPS analysis system development, including research on ion diffusion, enzymatic reaction, microbial metabolism, and droplet microfluidics.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Tatsuo Yoshinobu, Ko-ichiro Miyamoto
Summary: This study tested different waveforms of modulated illuminations for LAPS and found that a square wave with a low duty ratio was more efficient than a sine wave, maximizing the signal.
Review
Chemistry, Physical
Tatsuo Yoshinobu, Michael J. Schoening
Summary: The light-addressable potentiometric sensor (LAPS) is a versatile platform for chemical and biosensing, with the ability to divide its flat sensor surface into separate areas or pixels for independent sensing. It has been widely applied to cells and biological samples, with recent trends and applications focusing on advancements in technology and biomaterials.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Article
Electrochemistry
Jiezhang Luo, Shibin Liu, Jie Tan, Yinghao Chen, Wenbo Zhao, Huijuan Li, Guifang Li, Yongqian Du
Summary: In this study, a differential light addressable potentiometric sensor (LAPS) with solid-state reference (SSR) is proposed for pH measurement. By controlling the illumination excitation of SSR, the amplitude and phase between LAPS output and SSR output are matched, achieving signal matching and significant reduction of background signal. SSR, as a reference signal source, can assist the differential LAPS to realize low-background pH measurement conveniently and flexibly.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Jie Tan, Shibin Liu, Jiezhang Luo, Yinghao Chen, Wenbo Zhao, Huijuan Li, Guifang Li, Xueliang Li
Summary: In this study, a light addressable potentiometric sensor (LAPS) was proposed to detect local potential changes caused by protonation and deprotonation between electrolyte and insulator using light pulse. The semiconductor of LAPS was fabricated by combining microsphere lithography with wet etching, resulting in well-ordered, tunable and low-cost pyramidal pits-patterned silicon. The experiment results and theoretical analyses showed that LAPS with pyramidal pits-patterned silicon demonstrated acceptable pH response, good long-term stability, and high performance in terms of photocurrent enhancement ratio, signal-to-noise ratio and pH imaging.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Fangming Chen, Qian Guo, Qiaoyu Yang, Yao Meng, Mingrui Jiang, Jian Wang, De-Wen Zhang
Summary: Label-free electrochemical visualization of cancer cell apoptosis is achieved using a light-addressable electrochemical sensor. The study demonstrates that the AC photocurrent on apoptotic MCF-7 cells increases, indicating heterogeneity in apoptosis degrees. This new method provides potential applications in apoptosis-associated drug screening and efficacy evaluation.
ANALYTICAL CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Yun-Chong Li, Bo Jiao, Yi-Jun Zhang, Jian Wang, Wei Ren, De-Wen Zhang, Xun Hou, Zhao-Xin Wu
Summary: The authors demonstrate the potential of fullerene (C-60) as a candidate material for the photosensitive layer of LAPS, and propose a unique bipolar photo-response phenomenon. They explain this phenomenon with an oxygen-trap-induced space-charge mechanism.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Chemistry, Analytical
Yinghao Chen, Shibin Liu, Jiezhang Luo, Wenbo Zhao, Guifang Li, Yongqian Du, Jie Tan, Yue Tan, Yun Zhang
Summary: In this study, a crack metallic network (CMN) is proposed to improve the spatial resolution of a chemical imaging system based on light-addressable potentiometric sensor (LAPS). The CMN, prepared on a colloidal film as a template, effectively reduces electrode coverage and enhances photoelectric conversion efficiency.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Metallurgy & Metallurgical Engineering
Ko-ichiro Miyamoto, Manato Kaneko, Mengyun Wang, Carl Frederik Werner, Tatsuo Yoshinobu
Summary: In this study, a novel method for detecting hydrogen permeation through metal using a combination of LAPS and micro-volume measurement cell was proposed. The results demonstrated the potential of applying LAPS to visualize hydrogen permeation sites on metal surfaces.
ISIJ INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Jintao Liang, Kaiteng Yan, Yutong Liu, Xiaoqing Yao, Fei Guo, Wen Xue, Guiyin Li, Jiejing Chen, Zhide Zhou
Summary: In this study, a high-performance light addressable potentiometric sensor (LAPS) based on reduced graphene oxide-polyacrylamide-ferrocene/gold nanoparticles (rGO-PAM-Fc/AuNPs) was constructed for the detection of 1,5-AG. The designed LAPS showed good linearity, sensitivity, specificity, reproducibility, and stability. This work provides a highly sensitive, portable, and low-cost assay for the detection of 1,5-AG.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Jintao Liang, Xiaohang Shi, Huafu Feng, Min Chen, Wenzhan Li, Junxiang Lai, Weipeng Hu, Guiyin Li
Summary: A novel silicon-based light-addressable potentiometric sensor (LAPS) was designed for the detection of 1,5-anhydroglucitol (1,5-AG) in human serum, showing good sensitivity and specificity as a potential testing method for clinical samples.
BIOELECTROCHEMISTRY
(2021)
Review
Chemistry, Analytical
Yage Liu, Ping Zhu, Shuge Liu, Yating Chen, Dongxin Liang, Miaomiao Wang, Liping Du, Chunsheng Wu
Summary: This review introduces the basic principle and general configuration of the light-addressable potential sensor (LAPS), as well as its recent applications in chemical sensing, biosensing, and cell monitoring. The article outlines the development trends of LAPS from the aspects of material and optical improvement, aiming to provide research and application perspectives for chemical sensing, biosensing, and imaging technology.
Article
Chemistry, Analytical
Tao Liang, Nan Jiang, Shuqi Zhou, Xinyi Wang, Yingke Xu, Chunsheng Wu, Dmitry Kirsanov, Andrey Legin, Hao Wan, Ping Wang
Summary: This study combined LAPS with ionophore-doped all-solid-state silicone-rubber ISMs to establish a program-controlled multiplexed ISLAPS system for physiological ions detection. The silicone-rubber ISMs have better adhesion to silicon-based sensors without containing plasticizers, which can avoid the plasticizer pollution and improve the long-term stability. Pre-modifying a layer of P3OT on the sensor surface inhibit the formation of an aqueous layer and improve the sensor lifetime.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Fangming Chen, Qiaoyu Yang, MingRui Jiang, Yao Meng, Jian Wang
Summary: This study proposes a simple device that combines light-addressable potentiometric sensor (LAPS) imaging and microelectrodes for label-free sensing and imaging of electrochemical reactions. The device allows qualitative and quantitative observation and visualization of electrochemical reactions occurring on the microelectrodes.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biophysics
Nicole L. Walker, Jeffrey E. Dick
Summary: The development of a new electrochemical technique - open circuit potential, has been aimed at stable and reliable monitoring of biological metabolites. By immobilizing oxidase enzyme in chitosan hydrogel, the method achieves specificity in monitoring metabolites. This approach can effectively detect metabolites such as glucose and can be extended to detect other analytes.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Materials Science, Multidisciplinary
Denise Molinnus, Heiko Iken, Anna Lynn Johnen, Benjamin Richstein, Lena Hellmich, Arshak Poghossian, Joachim Knoch, Michael J. Schoening
Summary: Miniaturized electrolyte-insulator-semiconductor capacitors with ultrathin gate insulators were studied for their pH-sensitive sensor characteristics. Different combinations of gate insulator layer structures were characterized and it was found that SiO2-Ta2O5 stack showed the best overall sensor characteristics, while Si3N4-Ta2O5 stack had the highest accumulation capacitance and steepest slope in the capacitance-voltage curve among the studied systems.
PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE
(2022)
Article
Materials Science, Multidisciplinary
Tomoki Iwama, Yuanyuan Guo, Shoma Handa, Kumi Y. Inoue, Tatsuo Yoshinobu, Fabien Sorin, Hitoshi Shiku
Summary: This study introduces a novel fabrication method for fiber-based cBPE using thermal drawing, demonstrating the preparation and applicability of single-electrode and multielectrode fibers in ECL imaging with a functional rate of 99%. The work establishes a new mass-production approach for cBPE arrays and demonstrates magnified electrochemical imaging using a thermally-drawn electrode array fiber.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Chemistry, Analytical
Melanie Welden, Arshak Poghossian, Farnoosh Vahidpour, Tim Wendlandt, Michael Keusgen, Christina Wege, Michael J. Schoening
Summary: Utilizing tobacco mosaic virus particles as nanoscaffolds, a bi-enzyme EISCAP biosensor was developed for sequential detection of penicillin and urea. The biosensor exhibited high sensitivity and precision, and could also mimic an enzyme logic gate.
Article
Chemistry, Medicinal
Dua Ozsoylu, Torsten Wagner, Michael Josef Schoening
Summary: This review presents the technological advances and challenges of electrochemical cell-based biosensors, with a focus on recent research in the past five years. It covers topics such as cell-based biological field-effect devices, impedimetric sensors, and microelectrode arrays, as well as advancements in single-cell monitoring and integration with microfluidics. The article provides a comprehensive discussion on the future potential of electrochemical cell-based biosensors.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Denise Molinnus, Kevin A. Janus, Anyelina C. Fang, Aleksander Drinic, Stefan Achtsnicht, Marius Koepf, Michael Keusgen, Michael J. Schoening
Summary: This study presents the development of a biocompatible glucose biosensor using a fibroin substrate and a screen-printed carbon electrode. The electrode morphology and glucose sensitivity are analyzed, and the sensor shows great potential for in vivo and epidermal applications.
PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE
(2022)
Article
Chemistry, Analytical
Ko-ichiro Miyamoto, Rinya Hiramitsu, Carl Frederik Werner, Tatsuo Yoshinobu
Summary: Stainless steel is crucial in industry due to its resistance to corrosion. However, local corrosion can occur under specific conditions. In this study, a novel measurement system was developed to observe crevice corrosion, and real-time measurements of pH distribution and surface roughness were conducted using pH imaging and infrared light techniques. These measurements provide valuable insights into the propagation of crevice corrosion.
Article
Instruments & Instrumentation
Dua Oezsoylu, Beyza Karatellik, Michael J. Schoening, Torsten Wagner
Summary: Nowadays, biosensors are widely used in various fields and have become an integral part of our daily lives. To overcome the challenges in commercializing cell-based biosensors, a new method of cryopreserving cell monolayers in a ready-to-use setup has been introduced. Photopolymer-based magnetic composites have been developed to improve this method, allowing for controlled location, mobility, and orientation of the cells.
TM-TECHNISCHES MESSEN
(2022)
Article
Chemistry, Analytical
Tatsuo Yoshinobu, Ko-ichiro Miyamoto
Summary: This study tested different waveforms of modulated illuminations for LAPS and found that a square wave with a low duty ratio was more efficient than a sine wave, maximizing the signal.
Article
Chemistry, Analytical
Rino Nishimoto, Yuichi Sato, Jingxuan Wu, Tomoki Saizaki, Mahiro Kubo, Mengyun Wang, Hiroya Abe, Ines Richard, Tatsuo Yoshinobu, Fabien Sorin, Yuanyuan Guo
Summary: Bioelectronic devices are evolving from rigid to flexible materials, and thermally-drawn-fiber-based bioelectronics show promise due to their flexibility and integration of multi-functionalities. This study designed and fabricated microelectrode fibers made of carbon nanotube-based hybrid nanocomposites for electrochemical sensing. The fibers exhibited high sensitivity and long-term stability. In the future, multiple modalities can be integrated into a single fiber for various biological applications and health monitoring.
Article
Materials Science, Multidisciplinary
Kevin A. Janus, Stefan Achtsnicht, Laura Tempel, Aleksander Drinic, Alexander Kopp, Michael Keusgen, Michael J. Schoening
Summary: In this study, fibroin, polylactide (PLA), and carbon were investigated as biocompatible and biodegradable materials for amperometric biosensors. The carbon electrodes on fibroin and PLA substrates were modified with a glucose oxidase membrane and then encapsulated with the biocompatible material Ecoflex. Different curing parameters of the carbon electrodes were studied to determine their effect on the biosensor characteristics. The morphology of the electrodes was examined using scanning electron microscopy, and the biosensor performance was measured through amperometric measurements of glucose in a phosphate buffer solution.
PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Tobias Karschuck, Stefan Schmidt, Stefan Achtsnicht, Arshak Poghossian, Patrick Wagner, Michael Josef Schoening
Summary: Compared to single-analyte devices, multiplexed systems for multianalyte detection offer advantages such as reduced assay time and sample volume, low cost, and high throughput. In this study, a multiplexing platform was developed for the automated simultaneous characterization of multiple capacitive field-effect sensors using the capacitive-voltage (C-V) and constant-capacitance (ConCap) mode. The platform consists of a multicell arrangement with a common reference electrode, where the sensors are electrically connected to an impedance analyzer via a base station. The system was tested for pH measurements and the label-free detection of ligand-stabilized, charged gold nanoparticles, showing promising results.
PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE
(2023)
Article
Virology
Tim Wendlandt, Claudia Koch, Beate Britz, Anke Liedek, Nora Schmidt, Stefan Werner, Yuri Gleba, Farnoosh Vahidpour, Melanie Welden, Arshak Poghossian, Michael J. Schoening, Fabian J. Eber, Holger Jeske, Christina Wege
Summary: Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains of Staphylococcus aureus protein A (PA) were purified from plants. These particles could be loaded with antibodies and used for the immobilization of various substances. The TVCVPA carriers showed increased reusability compared to traditional methods, and the virus could be used for durable TVCVPA-assisted electrochemical biosensing.
Article
Chemistry, Analytical
Paulo V. Morais, Pedro H. Suman, Michael J. Schoening, Jose R. Siqueira Jr, Marcelo O. Orlandi
Summary: This study aims to fabricate a reusable and robust sensing unit on an electrolyte-insulator-semiconductor (EIS) device to detect heavy metal ions such as lead and nickel. The EIS-Sn3O4 sensor shows a higher affinity for Pb2+ ions with a sensitivity of approximately 25.8 mV/decade, suggesting its potential as a feasible capacitive field-effect sensor for heavy metal detection.
Article
Chemistry, Analytical
Morten Bertz, Denise Molinnus, Michael J. Schoening, Takayuki Homma
Summary: Hydrogen peroxide (H2O2) is widely used as a sterilization agent in aseptic food processing and medical applications. Little is known about the detailed mechanism of oxidative spore death by H2O2. This study investigates the chemical and morphological changes of Bacillus atrophaeus spores undergoing oxidative damage, revealing two distinct phases of spore death and the role of reactive oxygen species in the process.
Article
Biotechnology & Applied Microbiology
Dua Oezsoylu, Kevin A. Janus, Stefan Achtsnicht, Torsten Wagner, Michael Keusgen, Michael J. Schoening
Summary: Skin grafting is a common surgical procedure in dermatology, but failure rates are still high, causing significant burden. Real-time and objective monitoring of grafts is essential for evidence-based treatment. Advanced sensor applications using nanotechnology and material science can provide this monitoring.
SENSORS AND ACTUATORS REPORTS
(2023)
