Journal
PHYSICA MEDICA-EUROPEAN JOURNAL OF MEDICAL PHYSICS
Volume 26, Issue 4, Pages 233-239Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ejmp.2010.02.001
Keywords
Optical Tweezers; Raman microscopy; Optical stretching
Funding
- CNISM (Consorzio Nazionale Interuniversitario per le Scienze Fisiche della Materia)
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Raman confocal microscopy, combined with an optical stretcher, is used to study the spatial distribution and the oxidation state of hemoglobin in erythrocytes under stretching condition. In particular, a near infrared laser (lambda = 1064 nm) is used to generate multiple time-sharing Optical Tweezers to trap and stretch a single erythrocyte, while a second laser (lambda = 532 nm) acts as Raman probe. Our study demonstrates that stretching induces hemoglobin transition to the deoxygenated state. Moreover, by using Principal Component Analysis we prove the reversibility of the oxy bar right arrow deoxy hemoglobin transition after application of the optically induced mechanical stress. (C) 2010 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.
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