Article
Fisheries
Patrick Berrebi, Akos Horvath, Andrea Splendiani, Stefan Palm, Rafal Bernas
Summary: This study analyzed the genetic structure of 27 brown trout hatchery strains from Europe and North America, revealing the dominance of the North Atlantic lineage in European and American hatcheries, with the presence of local strains from domesticated regional wild fish. Most hatchery strains showed high to very high genetic polymorphism, likely due to genetic admixture counteracting losses of diversity from genetic drift and domestication. This study highlights the importance of identifying the genetic composition of hatchery stocks and the need for caution when stocking a common stock across a species' geographical range to avoid reducing genetic diversity and local adaptation.
Article
Environmental Sciences
Fernando Tuya, Nadine Schubert, Julio Aguirre, Daniela Basso, Eduardo O. Bastos, Flavio Berchez, Angelo F. Bernardino, Nestor E. Bosch, Heidi L. Burdett, Fernando Espino, Cindy Fernandez-Garcia, Ronaldo B. Francini-Filho, Patrick Gagnon, Jason M. Hall-Spencer, Ricardo Haroun, Laurie C. Hofmann, Paulo A. Horta, Nicholas A. Kamenos, Line Le Gall, Rafael A. Magris, Sophie Martin, Wendy A. Nelson, Pedro Neves, Irene Olive, Francisco Otero-Ferrer, Viviana Pena, Guilherme H. Pereira-Filho, Federica Ragazzola, Ana Cristina Rebelo, Claudia Ribeiro, Eli Rinde, Kathryn Schoenrock, Joao Silva, Marina N. Sissini, Frederico T. S. Tamega
Summary: Global marine conservation is hindered by an imbalance in research efforts and policy actions, which limits progress towards sustainability. Rhodolith beds, despite their global ecological importance and numerous ecosystem functions, remain disproportionately understudied compared to other coastal ecosystems. The lack of information about these habitats and their ecosystem services hampers effective conservation measures, especially considering the severe pressures and threats they face. Therefore, it is crucial to prioritize research on rhodolith beds, combat degradation, and protect biodiversity for the future.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Stephen Buckley, Karen Hardy, Fredrik Hallgren, Lucy Kubiak-Martens, Zydrune Miliauskiene, Alison Sheridan, Iwona Sobkowiak-Tabaka, Maria Eulalia Subira
Summary: Research shows that there was widespread consumption of seaweed, submerged aquatic plants, and freshwater plants in prehistoric Europe, which continued into the farming period and early Middle Ages. This discovery is crucial for understanding the past and recognizing forgotten resources in the present.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Manuel Anguita-Maeso, Carmen Haro, Juan A. Navas-Cortes, Blanca B. Landa
Summary: This study evaluated the effects of extraction methods, plant age, and PCR primers on the characterization of xylem bacterial communities. Different extraction methods and PCR primers resulted in significant biases in describing the bacterial community. The relative importance of dominant bacterial genera varied depending on the primers and extraction methods used.
Article
Biophysics
Yang Wang, Gaolian Xu, Mengdi Guan, Qingyang Zhang, Zhiying Wang, Wenhui Chen, Xiaoxiao Yang, Anbang Qiao, Yubo Fan, Xinxia Cai, Zhou Chen, Lingqian Chang, Bo Wei
Summary: An electrochemical quantitative polymerase chain reaction (E-PCR) platform was developed for multiplexed and quantitative analysis of single-based mutations (SbM) in limited and precious samples, achieving ultra-high sensitivity and specificity.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Microbiology
Jelena Pavlovic, Duccio Cavalieri, Giorgio Mastromei, Domenico Pangallo, Brunella Perito, Massimiliano Marvasi
Summary: The MinION single-molecule sequencing system has attracted the attention of microbiologists in the cultural heritage conservation community. Despite being a recent innovation, it has been applied on various substrates to identify species involved in substrate degradation. This technology is proving to be a useful and cost-effective tool for microbiologists and conservators in the conservation of artworks.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Optics
Vahid Ganjalizadeh, Aaron R. Hawkins, Holger Schmidt
Summary: Integrated optofluidic biosensors have achieved ultrahigh sensitivity for single particle detection and attomolar target concentrations. However, a wide dynamic range is often needed in practical applications, which can only be achieved by using multiple detection modalities or sacrificing linearity. In this study, a novel analysis technique using temporal excitation at two different time scales is demonstrated to enable simultaneous digital and analog detection of fluorescent targets. The technique enables seamless detection of nanobeads across eight orders of magnitude of concentration and multiplex analysis of fluorescent nanobead mixtures with vastly different concentrations. This advancement significantly improves the performance and versatility of integrated biosensors, especially for point-of-use applications.
Review
Genetics & Heredity
Gerardo Cazzato, Concetta Caporusso, Francesca Arezzo, Antonietta Cimmino, Anna Colagrande, Vera Loizzi, Gennaro Cormio, Teresa Lettini, Eugenio Maiorano, Vincenza Sara Scarcella, Paola Tarantino, Maricla Marrone, Alessandra Stellacci, Paola Parente, Paolo Romita, Aurora De Marco, Vincenzo Venerito, Caterina Foti, Giuseppe Ingravallo, Roberta Rossi, Leonardo Resta
Summary: Advancements in pathology have shifted towards molecular and genetic studies, allowing for the implementation of targeted therapies; however, issues may arise in NGS due to damage to genetic material in fixed samples.
Article
Biology
Hidehiko Kawai, Ren Iwata, Shungo Ebi, Ryusei Sugihara, Shogo Masuda, Chiho Fujiwara, Shingo Kimura, Hiroyuki Kamiya
Summary: This study presents an advanced method for mutation analysis using the supF gene. The method is simple, versatile, and cost-effective, and provides valuable insights into mutation frequencies and spectra. The results reveal previously undetected mutations and offer a novel perspective on the mechanisms of mutagenesis.
Article
Fisheries
Suzannah-Lynn Billing, Julie Rostan, Paul Tett, Adrian Macleod
Summary: This study aims to explore the social interactions of seaweed cultivation, finding that interpersonal relationships, perceptions of environmental risk, scale of decision-making and operations, and communication are key to local perceptions of seaweed cultivation operations. The concept of social license to operate is discussed as a framework for this emergent industry.
Article
Medicine, Research & Experimental
Pascal Pedini, Hajer Graiet, Laurine Laget, Lugdivine Filosa, Jade Chatron, Nicem Cherouat, Jacques Chiaroni, Lucas Hubert, Coralie Frassati, Christophe Picard
Summary: This study compared the efficiencies of four (semi)-automated cfDNA isolation instruments in isolating cfDNA and RhD-cffDNA, and found differences in DNA yield depending on the isolation procedure and quantification method used. The study suggests that the choice of pre-analytical isolation systems needs to be carefully validated in routine clinical practice.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Loetitia Favre, Nouhoum Sako, Sihem Tarfi, Violaine Tran Quang, Corine Joy, Aurelie Dupuy, Erell Guillerm, Philippe Gaulard, Orianne Wagner-Ballon, Anais Pujals, Ivan Sloma
Summary: This study compared two new multiplexed PCR assays for IDH1/2 mutation detection and found that the ddPCR assay had better analytical performances than the PGX assay, with high specificity, sensitivity, and correlation with NGS results. Therefore, the new highly multiplexed ddPCR is a fast and cost-effective assay that can be used to identify and follow cancer patients during anti-IDH1/2-targeted therapies.
MOLECULAR ONCOLOGY
(2022)
Review
Microbiology
Clara Valero, Maria Teresa Martin-Gomez, Maria Jose Buitrago
Summary: Diagnosing endemic mycoses remains challenging due to the limited availability of reliable diagnostic methods, lack of clinical suspicion in non-endemic areas, and limitations of conventional techniques. Molecular methods have emerged as promising tools for rapid diagnosis; however, the lack of consensus among laboratories and limited availability of commercial tests compromise their diagnostic effectiveness.
Article
Oncology
Elena V. Preobrazhenskaya, Alla U. Shleykina, Olga A. Gorustovich, Alexandr S. Martianov, Ilya V. Bizin, Elena I. Anisimova, Tatjana N. Sokolova, Svetlana A. Chuinyshena, Ekatherina Sh. Kuligina, Alexandr V. Togo, Alexey M. Belyaev, Alexandr O. Ivantsov, Anna P. Sokolenko, Evgeny N. Imyanitov
Summary: PALB2 germ-line mutations contribute to a small fraction of cancer cases in Russia. The majority although not all PALB2-driven breast cancers have somatic inactivation of the remaining PALB2 allele and therefore potential sensitivity to platinum compounds and PARP inhibitors.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Review
Clinical Neurology
Hongyan Xu, Peng Chen, Shihan Guo, Xiaokai Shen, Yun Lu
Summary: In recent years, the etiological diagnosis of viral encephalitis has made significant progress with the rapid development of molecular biology techniques such as polymerase chain reaction and molecular biochip. Currently, the etiological examination of viral meningitis mainly includes virus isolation, serological detection, and molecular biological nucleic acid detection. This article reviews the progress in etiological diagnosis of viral meningitis.
FRONTIERS IN NEUROLOGY
(2023)
Article
Plant Sciences
Karen Filbee-Dexter, Thomas Wernberg, Rodolfo Barreiro, Melinda A. Coleman, Thibaut Bettignies, Colette J. Feehan, Joao N. Franco, Berit Hasler, Ines Louro, Kjell M. Norderhaug, Peter A. U. Staehr, Fernando Tuya, Jan Verbeek
Summary: The urgent need for ecosystem restoration has prompted the international community to accelerate the efforts, and seaweed forest restoration has lagged behind other ecosystems. Its transformation into a commercial-scale enterprise can contribute significantly to global restoration efforts.
JOURNAL OF PHYCOLOGY
(2022)
Article
Plant Sciences
Ruben Portela, Rodolfo Barreiro, Sergio R. Roiloa
Summary: In coastal sand dunes, sand burial poses a common stress to plant performance. Clonal plants, like Carpobrotus edulis, exhibit reduced survival and growth in apical ramets when subjected to sand burial, while basal ramets show a compensatory response. This study demonstrates the importance of considering modular plasticity in understanding plant responses to sand burial stress.
Article
Environmental Sciences
Sara Barrientos, Cristina Pineiro-Corbeira, Rodolfo Barreiro
Summary: Kelp forests around the world have been declining, mainly due to climate change. This study reveals that excessive herbivory by fish has caused the degradation of golden kelp forests in NW Spain.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biodiversity Conservation
Sergio R. Roiloa, Fei-Hai Yu, Rodolfo Barreiro
Summary: The application of glyphosate to the invasive clonal plant Carpobrotus edulis not only negatively affects its own growth and photochemical efficiency, but also reduces the growth of untreated basal ramets connected to the treated apical ramets. Localized glyphosate application can effectively reduce the amount of herbicide used and minimize its negative impact on ecosystems.
Article
Plant Sciences
Sara Barrientos, Rodolfo Barreiro, Cristina Pineiro-Corbeira
Summary: Kelp forests, one of the most productive ecosystems in the world, have been declining due to climate change and non-climatic stressors such as grazing. Marine protected areas have been suggested as a strategy to boost ecosystem resilience, but their effectiveness may vary for different components of the local community.
EUROPEAN JOURNAL OF PHYCOLOGY
(2023)
Article
Oceanography
Raquel De la Cruz-Modino, Cristina Pineiro-Corbeira, Josue Gutierrez-Barroso, Carla Gonzalez-Cruz, Rodolfo Barreiro, Jose A. Batista-Medina, Jose J. Pascual-Fernandez, Jose A. Gonzalez, Agustin Santana-Talavera, Shankar Aswani
Summary: This paper explores the impact of a volcanic eruption on a fishing community in El Hierro, Spain, and the strategies used by the local population to cope with it. The study reveals that the combination of various activities and institutional support enhanced the community's resilience and ensured access to important marine resources and recreational/cultural services.
OCEAN & COASTAL MANAGEMENT
(2022)
Article
Plant Sciences
Josefina G. Campoy, Mar Sobral, Belen Carro, Margarita Lema, Rodolfo Barreiro, Ruben Retuerto
Summary: Through a field experiment, we investigated the impact of the projected climate change in Southern Europe on global DNA methylation and phenotypic variation in individuals of the clonal plant C. edulis. Our results showed that changes in temperature and rainfall affected phenotypic differences among plants, but not global DNA methylation levels. Interestingly, individuals from the Iberian Peninsula exhibited higher levels of global methylation than their native counterparts from South Africa. The study also revealed differences in leaf traits between native and invasive phenotypes, suggesting an intraspecific functional variation during the process of invasion. These findings highlight the importance of epigenetic plasticity in the rapid adaptation of invasive clonal plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Nadine Schubert, Viviana Pena, Vinicius W. Salazar, Paulo A. Horta, Pedro Neves, Claudia Ribeiro, Francisco Otero-Ferrer, Fernando Tuya, Fernando Espino, Kathryn Schoenrock, Laurie C. Hofmann, Line Le Gall, Rui Santos, Joao Silva
Summary: The study investigates the photosynthetic and calcification mechanisms of different species of Atlantic rhodoliths, finding significant differences and influences from local environmental conditions.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Environmental Sciences
Cristina Pineiro-Corbeira, Sara Barrientos, Rodolfo Barreiro, Raquel de la Cruz-Modino
Summary: Kelp forests are declining worldwide, but their consequences for small-scale fisheries have been overlooked. This study conducted interviews with fishermen in NW Spain to gather local ecological knowledge about kelp forests, highlighting their economic importance and decline.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Environmental Sciences
Cristina Fernandez, Cristina Pineiro-Corbeira, Sara Barrientos, Rodolfo Barreiro
Summary: The decline of L. ochroleuca in NW Iberia may alter the patterns of biodiversity within kelp forests, as the coexisting S. polyschides cannot fully replace it as a habitat provider for holdfast epifauna.
MARINE ENVIRONMENTAL RESEARCH
(2022)
Article
Biodiversity Conservation
Ines Perez-Peris, Sandra Navarro-Mayoral, Marcial Cosme de Esteban, Fernando Tuya, Viviana Pena, Ignacio Barbara, Pedro Neves, Claudia Ribeiro, Antonio Abreu, Jacques Grall, Fernando Espino, Nestor Echedey Bosch, Ricardo Haroun, Francisco Otero-Ferrer
Summary: This study examined the impact of depth on the size and morphology of calcareous red macroalgae structures (rhodoliths) and the abundance of associated flora and fauna. The findings revealed that depth typically influenced the size and shape of rhodoliths, with larger nodules found at intermediate and deep depths. The biomass of attached macroalgae (epiphytes) also increased with depth. The study concluded that the morphology of rhodoliths and associated epibionts were primarily influenced by depth, regardless of latitude.
Article
Plant Sciences
Paul W. Gabrielson, Gavin W. Maneveldt, Jeffery R. Hughey, Viviana Pena
Summary: Phylogenetic analyses of rbcL gene sequences and concatenated rbcL, psbA, and nuclear SSU rRNA gene sequences identified Lithothamnion muelleri and three other southern Australian species as the generitype of the genus Lithothamnion. Cold water boreal species previously classified in Lithothamnion were transferred to the newly described genus Boreolithothamnion, with B. glaciale as the generitype. The discussion emphasizes the importance of using phylogenetic analyses to understand and correctly classify non-geniculate corallines based on morpho-anatomical characters.
JOURNAL OF PHYCOLOGY
(2023)
Article
Plant Sciences
Paul W. Gabrielson, Jeffery R. Hughey, Viviana Pena, Joseph L. Richards, Gary W. Saunders, Brenton Twist, Tracy Farr, Wendy A. Nelson
Summary: To resolve the uncertainty around taxonomic classification of Sporolithon species, targeted PCR sequencing was conducted on historical and recently collected specimens. The results confirmed the existence of distinct species and identified previously misclassified specimens. DNA sequencing of type specimens of coralline species was shown to be the most reliable method for accurate naming.
Article
Environmental Sciences
Fernando Tuya, Nadine Schubert, Julio Aguirre, Daniela Basso, Eduardo O. Bastos, Flavio Berchez, Angelo F. Bernardino, Nestor E. Bosch, Heidi L. Burdett, Fernando Espino, Cindy Fernandez-Garcia, Ronaldo B. Francini-Filho, Patrick Gagnon, Jason M. Hall-Spencer, Ricardo Haroun, Laurie C. Hofmann, Paulo A. Horta, Nicholas A. Kamenos, Line Le Gall, Rafael A. Magris, Sophie Martin, Wendy A. Nelson, Pedro Neves, Irene Olive, Francisco Otero-Ferrer, Viviana Pena, Guilherme H. Pereira-Filho, Federica Ragazzola, Ana Cristina Rebelo, Claudia Ribeiro, Eli Rinde, Kathryn Schoenrock, Joao Silva, Marina N. Sissini, Frederico T. S. Tamega
Summary: Global marine conservation is hindered by an imbalance in research efforts and policy actions, which limits progress towards sustainability. Rhodolith beds, despite their global ecological importance and numerous ecosystem functions, remain disproportionately understudied compared to other coastal ecosystems. The lack of information about these habitats and their ecosystem services hampers effective conservation measures, especially considering the severe pressures and threats they face. Therefore, it is crucial to prioritize research on rhodolith beds, combat degradation, and protect biodiversity for the future.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Geosciences, Multidisciplinary
Sofie Vranken, Marine Robuchon, Stefanie Dekeyzer, Ignacio Barbara, Inka Bartsch, Aurelie Blanfune, Charles-Francois Boudouresque, Wim Decock, Christophe Destombe, Bruno de Reviers, Pilar Diaz-Tapia, Anne Herbst, Romain Julliard, Rolf Karez, Priit Kersen, Stacy A. Krueger-Hadfield, Ralph Kuhlenkamp, Akira F. Peters, Viviana Pena, Cristina Pineiro-Corbeira, Fabio Rindi, Florence Rousseau, Jan Rueness, Hendrik Schubert, Kjersti Sjotun, Marta Sanson, Dan Smale, Thierry Thibaut, Myriam Valero, Leen Vandepitte, Bart Vanhoorne, Alba Verges, Marc Verlaque, Christophe Vieira, Line Le Gall, Frederik Leliaert, Olivier De Clerck
Summary: The study focuses on analyzing the biological and ecological traits of European seaweeds and organizing the data within a standardized framework. A trait database containing 45,175 records for 21 traits was created, providing valuable information for future research on seaweed diversity, evolution, and response to global changes. The data are publicly accessible and will contribute to the development of ecosystem models and marine conservation policies.
EARTH SYSTEM SCIENCE DATA
(2023)
