Journal
PHOTOSYNTHESIS RESEARCH
Volume 104, Issue 2-3, Pages 275-282Publisher
SPRINGER
DOI: 10.1007/s11120-009-9508-1
Keywords
Plant photosystems; Photo-CIDNP; Electron transfer; Solid-state NMR
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Funding
- Netherlands Organization for Scientific Research (NWO) [700.53.423]
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Cyanobacteria are widely used as model organism of oxygenic photosynthesis due to being the simplest photosynthetic organisms containing both photosystem I and II (PSI and PSII). Photochemically induced dynamic nuclear polarization (photo-CIDNP) C-13 magic-angle spinning (MAS) NMR is a powerful tool in understanding the photosynthesis machinery down to atomic level. Combined with selective isotope enrichment this technique has now opened the door to study primary charge separation in whole living cells. Here, we present the first photo-CIDNP observed in whole cells of the cyanobacterium Synechocystis.
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