Journal
PHOTOSYNTHESIS RESEARCH
Volume 104, Issue 2-3, Pages 221-231Publisher
SPRINGER
DOI: 10.1007/s11120-009-9523-2
Keywords
Light-harvesting unit; (13)C-labeled chlorosome; Supramolecular structure; Proton-driven spin diffusion; Simulated anneal molecular dynamics; Piggyback-dimer
Categories
Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Japan New Energy and Industrial Technology Development Organization
Ask authors/readers for more resources
Green sulfur photosynthetic bacteria optimize their antennas, chlorosomes, especially for harvesting weak light by organizing bacteriochlorophyll (BChl) assembly without any support of proteins. As it is difficult to crystallize the organelles, a high-resolution structure of the light-harvesting devices in the chlorosomes has not been clarified. We have determined the structure of BChl c assembly in the intact chlorosomes from Chlorobium limicola on the basis of (13)C dipolar spin-diffusion solid-state NMR analysis of uniformly (13)C-labeled chlorosomes. About 90 intermolecular C-C distances were obtained by the simultaneous assignment of distance correlations and the structure optimization preceded by the polarization-transfer matrix analysis. An atomic structure was obtained, using these distance constraints. The determined structure of the chlorosomal BChl c assembly is built with the parallel layers of piggyback-dimers. This supramolecular structure would provide insights into the mechanism of weak-light capturing.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available