Journal
PHOTOSYNTHESIS RESEARCH
Volume 102, Issue 2-3, Pages 415-425Publisher
SPRINGER
DOI: 10.1007/s11120-009-9478-3
Keywords
Magic angle spinning NMR; LH2 complex; Electronic structure; Membrane protein; Isotope labeling
Categories
Ask authors/readers for more resources
In the last two decades, Magic Angle Spinning (MAS) NMR has created its own niche in studies involving photosynthetic membrane protein complexes, owing to its ability to provide structural and functional information at atomic resolution of membrane proteins when in the membrane, in the natural environment. The light-harvesting two (LH2) transmembrane complex from Rhodopseudomonas acidophila is used to illustrate the procedure of the technique applicable in photosynthesis research. One- and two-dimensional solid-state NMR experiments involving C-13- and N-15-labeled LH2 complexes allow to make a sequence-specific assignment of NMR signals, which forms the basis for resolving structural details and the assessment of charge transfer, electronic delocalization effects, and functional strain in the ground state.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available