Article
Biochemical Research Methods
Maximilian Emanuel Blaha, Sadat Hasan, Christian Dusny, Detlev Belder
Summary: This study presents a label-free sorting technique for cyanobacterial cells based on their fluorescence lifetime, which enables single-cell sensitivity sorting and can be used to study the effects of stressors on cyanobacterial physiology.
Article
Biology
Katsunori Yoshikawa, Kenichi Ogawa, Yoshihiro Toya, Seiji Akimoto, Fumio Matsuda, Hiroshi Shimizu
Summary: A high light tolerant strain of cyanobacteria was obtained through adaptive laboratory evolution, showing no growth inhibition under conditions up to 9000 μmol m(-2) s(-1) and exhibiting an increase in isiA expression.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Bin Lai, Hans Schneider, Jenny Tschoertner, Andreas Schmid, Jens O. Kroemer
Summary: Biophotovoltaics (BPV) offers a promising solution for hydrogen supply by coupling light-driven water splitting to hydrogen formation using oxygenic photoautotrophic cyanobacteria. However, current limitations in photon-to-current efficiency and experimental equipment hinder research progress. By developing a new technical-scale BPV platform with optimized parameters and identifying interfering factors, reproducible and stable current output signals were achieved. The use of an optimized nBG11 medium helped minimize background interferences, allowing for reliable and quantitative investigation, optimization, and scale-up of BPV processes.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Physical
Pawel Zbik, Kinga Klodawska, Przemyslaw Malec
Summary: In this study, myxoxanthophyll glycosides were isolated from cyanobacteria and found to exist as a mixture of geometrical isomers. The absorption maxima of myxoxanthophyll were influenced by solvent properties. Organic solvents showed no specific interactions with myxoxanthophyll, while an aqueous environment promoted intermolecular interactions. The biological significance of these observations remains to be determined.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biotechnology & Applied Microbiology
Gao Chen, Yuelei Cao, Huairong Zhong, Xiaodong Wang, Yanle Li, Xiaoyan Cui, Xiaoyuan Lu, Xiangdong Bi, Meixue Dai
Summary: Serine/threonine kinases (STKs) are crucial for prokaryotic cellular functions, and in this study, the roles of the STK genes spkD and spkG in fatty acid biosynthesis of Synechocystis sp. PCC6803 were investigated. Knocking out spkD and spkG genes led to lower levels of certain fatty acids and altered expression of fatty acid desaturase genes, indicating their importance in polyunsaturated fatty acid biosynthesis and potential for genetic engineering in cyanobacteria.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xiaomeng Tong, Eui-Jin Kim, Jeong K. Lee
Summary: This study found that thylakoid membranes can generate NADPH as a reducing power source under light. However, they can also generate singlet oxygen, causing oxidative damage. By managing the formation of reactive oxygen species and removing hydrogen peroxide, the sustainability of thylakoid membranes can be improved.
MICROBIAL CELL FACTORIES
(2022)
Article
Plant Sciences
Takako Ogawa, Kenta Suzuki, Kintake Sonoike
Summary: This study demonstrates the significant impact of NADPH redox state on chlorophyll fluorescence induction in cyanobacteria. The interaction between photosynthesis and respiration through NADPH provides a simple method for monitoring chlorophyll fluorescence induction in cyanobacteria.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemical Research Methods
Beatrice Battaglino, Wei Du, Cristina Pagliano, Joeri A. Jongbloets, Angela Re, Guido Saracco, Filipe Branco dos Santos
Summary: Cyanobacteria, powered by sunlight, have the potential to convert atmospheric CO2 into valuable compounds while releasing O2, making them promising candidates for microbial cell factories. By utilizing growth-coupled metabolites and thermodynamically favored conversions, the scope of candidate compounds for production can be expanded, leading to increased stability and productivity of engineered strains.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Engineering, Environmental
Linlin Cui, Ling Fan, Zhanfei Li, Junjun Wang, Ran Chen, Yejuan Zhang, Jinju Cheng, Xueling Wu, Jiaokun Li, Huaqun Yin, Weiming Zeng, Li Shen
Summary: The absence of EPS on the cell surface of cyanobacteria reduces the adsorption amount to heavy metal ions, with heavy metal ions interacting with polysaccharides before proteins on cyanobacteria cells.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Microbiology
Saul A. Lema, Marina Klemencic, Franziska Vollmy, Maarten Altelaar, Christiane Funk
Summary: Caspases are proteases involved in the execution of apoptosis in animals. While structural homologs are found in other organisms, their functional homology is still disputed. Pseudo-caspases and pseudo-metacaspases have been shown to play a role in cell death programs.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Yu-Feng Zhai, Rui-Hang Xu, Zheng-Fei Yang, Xiao-Ping Chi, Shuang-Yan Wei, Pei-Min He, Rui Jia
Summary: The study investigated the efficacy of trans-vp28 gene Synechocystis sp. PCC6803 on Penaeus vannamei infected with white spot syndrome virus (WSSV). The results revealed that the experimental group showed a significantly higher survival rate after challenge, higher immune-related enzyme activities in tissues, and more normal tissue structure based on histopathology analysis.
Article
Marine & Freshwater Biology
Yao Tao, Man He, Beibei Chen, Gang Ruan, Pingping Xu, Yixue Xia, Gaofei Song, Yonghong Bi, Bin Hu
Summary: With the development of single cell analysis techniques, the concept of precision toxicology has been proposed to perform toxicological assessments on individual cells due to their heterogeneity. This study investigated the biosorption and bioaccumulation of Cd in Synechocystis sp. PCC 6803 using a droplet microfluidic device combined with inductively coupled plasma mass spectrometry detection. The toxicological responses of the algae cell to Cd2+ exposure were also studied. The results revealed the heterogeneity in cellular uptake of Cd2+ and showed that Cd accumulation in algal cells had toxic effects through oxidative stress.
AQUATIC TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hiroyuki Ashida, Kaho Murakami, Kenji Inagaki, Yoshihiro Sawa, Hisashi Hemmi, Yugo Iwasaki, Tohru Yoshimura
Summary: Alanine racemase is an important enzyme involved in bacterial cell wall synthesis. The cyanobacterial enzyme shows characteristics of both gram-negative and gram-positive bacteria. Our study revealed that the enzyme from Synechocystis sp. PCC6803 exhibits similar enzymatic properties to other bacterial enzymes, with a conserved amino acid residue in the active center being replaced with a different residue. This study contributes to our understanding of bacterial enzymes.
JOURNAL OF BIOCHEMISTRY
(2022)
Article
Plant Sciences
Jun Minagawa, Marcel Dann
Summary: Cyanobacteria mainly use bicarbonate ions to absorb hydrated CO2 for carbon assimilation, with the help of carboxysomal carbonic anhydrase (CA) enzymes. Some cyanobacterial genomes also encode extracellular CAs, but their exact role is still largely unknown. CahB1 is the only known active extracellular beta-CA in cyanobacteria, but it is likely a carboxysomal protein rather than a secreted one. When expressed in Synechocystis sp. PCC6803, CahB1 was found to complement the loss of endogenous CA and localize to a carboxysome-harboring and CA-active cell fraction, indicating its retention of crucial carboxysomal CA properties.
Article
Environmental Sciences
Qinghua Tian, Junjun Wang, Linlin Cui, Weimin Zeng, Guanzhou Qiu, Qi Hu, Anan Peng, Du Zhang, Li Shen
Summary: This study discusses the adsorption behavior, cytotoxicity, and stress response mechanism of microalgae under cadmium stress. Through RNAseq and enrichment analysis, the study reveals the gene expression patterns and key genes in microalgae responding to cadmium stress. The study provides detailed short-term and long-term genomic information on microalgae and suggests potential targets for algae-mediated genetic engineering.
Article
Plant Sciences
Satoko Iida, Miyuki Ikeda, Momoe Amano, Hidetoshi Sakayama, Yasuro Kadono, Keiko Kosuge
JOURNAL OF PLANT RESEARCH
(2016)
Article
Plant Sciences
Satoko Iida, Minako Ashiya, Yasuro Kadono
Article
Plant Sciences
Momoe Amano, Satoko Iida, Keiko Kosuge
Article
Genetics & Heredity
Satoko Iida, Atsushi Kobiyama, Takehiko Ogata, Akio Murakami
Article
Plant Sciences
Satoko Iida, Atsushi Kobiyama, Takehiko Ogata, Akio Murakami
PLANT AND CELL PHYSIOLOGY
(2010)
Article
Plant Sciences
Satoko Iida, Yasuro Kadono, Keiko Kosuge
PLANT SPECIES BIOLOGY
(2013)
Article
Multidisciplinary Sciences
Satoko Iida, Atsuko Miyagi, Seishiro Aoki, Motomi Ito, Yasuro Kadono, Keiko Kosuge
Article
Multidisciplinary Sciences
Keiko Kosuge, Satoko Iida, Kiyoshi Katou, Tetsuro Mimura
SCIENTIFIC REPORTS
(2013)
Article
Plant Sciences
Satoko Iida, Ayumi Yamada, Momoe Amano, Jun Ishii, Yasuro Kadono, Keiko Kosuge
JOURNAL OF PLANT RESEARCH
(2007)
Article
Plant Sciences
S Iida, Y Kadono
JOURNAL OF PLANT RESEARCH
(2002)
Article
Plant Sciences
S Iida, Y Kadono