Article
Biochemical Research Methods
Panagiotis Giamougiannis, Camilo L. M. Morais, Rita Grabowska, Katherine M. Ashton, Nicholas J. Wood, Pierre L. Martin-Hirsch, Francis L. Martin
Summary: The study compared the performance of blood plasma, serum, and ascitic fluid in ovarian cancer detection using Raman microspectroscopy. Ascitic fluid showed the best class separation in both unsupervised and supervised discrimination approaches, with higher classification accuracies, sensitivities, and specificities compared to plasma or serum. The presence of collagen information in ascitic fluid was found to be responsible for distinguishing ovarian cancer samples, making it a potential diagnostic method for ovarian cancer.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Mei-Huan Wang, Xiao Liu, Qian Wang, Hua-Wei Zhang
Summary: This study investigated the diagnostic efficiency of Raman spectroscopy for breast cancer. The meta-analysis results showed that Raman spectroscopy has high sensitivity and specificity for breast cancer diagnosis and may be an effective and accurate tool.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xingkang Diao, Xinli Li, Shuping Hou, Haijuan Li, Guohua Qi, Yongdong Jin
Summary: Exosomes are promising noninvasive biomarkers for the early diagnosis and treatment of diseases, especially cancer. However, accurately distinguishing exosomes from clinical samples remains a challenge due to their heterogeneity. In this study, a machine learning-based label-free surface-enhanced Raman spectroscopy method was used to accurately identify exosomes in human serum samples for the diagnosis of breast cancer and cervical cancer.
ANALYTICAL CHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Kunxiang Liu, Qi Zhao, Bei Li, Xia Zhao
Summary: This article reviews the applications of Raman spectroscopy for in vivo and in vitro diagnosis of gastric cancer, methodology related to spectroscopy data analysis, and presents the limitations of the technique.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Javier Garcia-Alonso, David Maestre, Emilio Nogales, Ana Cremades, Bianchi Mendez
Summary: In this study, NiGa2O4 microneedles with variable morphology and dimensions were successfully synthesized by an autocatalytic Vapor-Liquid-Solid process. The crystallographic facets and inversion degrees of the spinel structure were found to vary with the temperatures employed during the synthesis. The high crystallinity and preferred orientation of the structures allowed for the study of Raman vibrational modes and the observation of defect-related and near-bandgap emissions in photoluminescence measurements.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Dermatology
Ivan A. Bratchenko, Lyudmila A. Bratchenko, Alexander A. Moryatov, Yulia A. Khristoforova, Dmitry N. Artemyev, Oleg O. Myakinin, Andrey E. Orlov, Sergey V. Kozlov, Valery P. Zakharov
Summary: In this study, skin cancer was diagnosed in vivo using a portable low-cost spectroscopy setup, showing promising accuracy in classifying malignant and benign tumors, melanomas and pigmented tumors, and melanomas and seborrhoeic keratosis. The proposed system's automatic analysis accuracy outperformed GPs and trainees, and was comparable to trained dermatologists, suggesting potential for improving neoplasms classification in combination with other optical techniques.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xien Yang, Zhongyu Wu, Quanhong Ou, Kai Qian, Liqin Jiang, Weiye Yang, Youming Shi, Gang Liu
Summary: This study investigated the serum samples of patients with lung cancer and healthy individuals using a combination of data fusion and wavelet transform. Results showed that the combination of FTIR spectroscopy and Raman spectroscopy based on data fusion and wavelet transform can effectively diagnose lung cancer patients.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xinyue Chen, Sofian Tijono, Bridget Tsai, Lawrence William Chamley, Lai-Ming Ching, Qi Chen
Summary: The biological connection between cancer and pregnancy is of interest because of the similar mechanisms between tumour and placental cells. Placental extracellular vesicles (EVs) may play a role in regulating the proliferation and invasion of placental cells, but the understanding of this regulation is limited. In this study, placental EVs were found to inhibit ovarian tumour growth in a xenograft model by inducing cellular necrosis and infiltrating CD169+ macrophages and NK cells into the tumour tissues.
BIOSCIENCE REPORTS
(2023)
Article
Chemistry, Analytical
Jung Bin Phyo, Ayoung Woo, Ho Jae Yu, Kyongmook Lim, Baek Hwan Cho, Ho Sang Jung, Min-Young Lee
Summary: Metabolomics has shown great potential for early cancer diagnosis, and a rapid urine analysis system based on SERS has been developed for the diagnosis of pancreatic cancer and prostate cancer. The results indicate that the system has great potential for noninvasive cancer diagnosis and screening.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Adam D. Morris, Daniel L. D. Freitas, Kassio M. G. Lima, Lauren Floyd, Mark E. Brady, Ajay P. Dhaygude, Anthony W. Rowbottom, Francis L. Martin
Summary: This study evaluated the application of machine learning and Raman spectroscopy for the automated computational detection of disease activity in ANCA-associated glomerulonephritis. The results showed that spectral data from tissue samples were able to accurately discriminate disease activity, while corresponding spectrochemical changes in paired urine samples were limited.
Review
Biotechnology & Applied Microbiology
Ting Lin, Ya-Li Song, Pu Kuang, Si Chen, Zhigang Mao, Ting-Ting Zeng
Summary: The optical technology of Surface-enhanced Raman scattering (SERS) plays a significant role in cancer diagnosis, with its performance being highly dependent on the properties and morphology of the substrate. SERS technology offers advantages of rapidity, high specificity, and high sensitivity.
Article
Medicine, Research & Experimental
Conor C. Horgan, Mads S. Bergholt, Anika Nagelkerke, May Zaw Thin, Isaac J. Pence, Ulrike Kauscher, Tammy L. Kalber, Daniel J. Stuckey, Molly M. Stevens
Summary: This article presents an alternative nanoparticle-free theranostic approach based on Raman spectroscopy and photodynamic therapy, which offers significant advantages and application prospects in clinical cancer treatment.
Article
Biochemistry & Molecular Biology
Tudor Moisoiu, Stefania D. Iancu, Dan Burghelea, Mihnea P. Dragomir, Gheorghita Iacob, Andrei Stefancu, Ramona G. Cozan, Oana Antal, Zoltan Balint, Valentin Muntean, Radu I. Badea, Emilia Licarete, Nicolae Leopold, Florin I. Elec
Summary: This study demonstrates the potential of surface-enhanced Raman scattering (SERS) profiling of serum as a liquid biopsy strategy for detecting renal cell carcinoma (RCC). The SERS analysis successfully distinguished RCC patients from controls and suggests the usefulness of SERS as a diagnostic and screening tool for RCC.
Article
Chemistry, Analytical
M. Czaplicka, A. A. Kowalska, A. B. Nowicka, D. Kurzydlowski, Z. Gronkiewicz, A. Machulak, W. Kukwa, A. Kaminska
Summary: This study demonstrates the significant potential of SERS combined with multivariate analysis for distinguishing carcinoma or tumor from normal salivary gland tissues as a rapid, label-free tool in cancer detection. PCA and PLS-DA methods were used to identify differences and classify salivary gland homogenates, showing promise for differentiation between healthy and tumor tissues.
ANALYTICA CHIMICA ACTA
(2021)
Article
Nanoscience & Nanotechnology
Ting Lan, Yang Zhao, Yu Du, Chunyi Ma, Rui Wang, Qianlei Zhang, Shanshan Wang, Wenxian Wei, Honghua Yuan, Qingli Huang
Summary: A novel SiO2 encapsulated Au star@AgAu yolk-shell nanostructure targeting CYPA was synthesized and used for early diagnosis and therapy of ovarian cancer. Spectroscopic and microscopic methods were used to investigate the morphology of the yolk-shell nanostructure, and in vitro and in vivo experiments were conducted. The as-prepared yolk-shell nanostructure showed excellent SERS performance and photothermal performance, making it an ideal candidate for specific sensitivity analysis and photothermal therapy of ovarian cancer in one platform.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Instruments & Instrumentation
Arti Hole, Gunjan Tyagi, Atul Deshmukh, Raviraj Deshpande, Vikram Gota, Pankaj Chaturvedi, C. Murali Krishna
Summary: Saliva, as a clinically informative bio-fluid with easy and multiple sample collection advantages, has recently been explored using optical techniques. Different sampling methods of saliva need to be standardized to prevent loss of analytes in processing. Studies comparing four different saliva sampling methodologies showed that pellet and supernatant exhibit discrete spectroscopic features useful for cancer diagnosis, while air-dried and lyophilized forms retain majority spectroscopic features of whole saliva and are less prone to sampling losses. This study indicates the potential utility of minimally invasive salivary Raman-based diagnostics in oral cancers.
APPLIED SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Keita Iwasaki, Asuka Araki, C. Murali Krishna, Riruke Maruyama, Tatsuyuki Yamamoto, Hemanth Noothalapati
Summary: Raman spectroscopy has the potential to improve accuracy of cytological and histopathological diagnosis. This study identified five pure biomolecular spectra, with lipid components potentially serving as a basis for discrimination. The technique could become an excellent cytodiagnostic tool for distinguishing breast cancer from normal cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Siddhita A. Jadhav, Pullithadathil Biji, Manoj Kumar Panthalingal, Murali C. Krishna, S. Rajkumar, Dattatraya S. Joshi, Natarajan Sundaram
Summary: The COVID-19 pandemic has highlighted the urgent need for a swift, sensitive, and inexpensive diagnostic protocol. The authors propose a new diagnostic approach based on Surface-enhanced Raman Spectroscopy (SERS) coupled with microfluidic devices to trap and identify viruses from diverse biological fluids. If successful, this approach could serve as a valuable tool for mass screening of COVID-19 and future viral pandemics.
MEDICAL HYPOTHESES
(2021)
Article
Biochemistry & Molecular Biology
Manik N. Waghmare, Tazeen S. Qureshi, C. Murali Krishna, Kshama Pansare, Nikhil Gadewal, Arti Hole, Prabhakar M. Dongre
Summary: The study investigated the influence of the protein-nanoparticle interface on drug binding and stability, demonstrating that the conjugation of whey protein with gold nanoparticles can enhance the binding affinity of anticancer drugs. The interaction mechanisms between drugs and the protein-nanoparticle conjugates were explored, providing insights into the selective conformations induced by drug binding.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Engineering, Biomedical
Atanu Bhattacharjee, Arti Hole, Akshat Malik, Aditi Sahu, S. P. Singh, Atul Deshmukh, Sudhir Nair, Pankaj Chaturvedi, C. Murali Krishna
Summary: The study investigates the potential of Raman spectroscopy in predicting disease-free survival in oral cancer patients, with 76 spectral points identified to accurately predict DFS in oral squamous cell carcinoma. The performance of spectral points was validated through Brier value, indicating a close approximation to actual progression.
LASERS IN MEDICAL SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Radhika Poojari, Mithila Bhujbal, Arti Hole, C. Murali Krishna
Summary: This study utilized Raman spectroscopy to monitor the response of combinatorial therapeutics in HCC tumor tissues of mice, demonstrating the potential for therapeutic monitoring.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2021)
Article
Spectroscopy
Anju Shrivastava, Lalit Mohan Aggarwal, Chilakapati Murali Krishna, Satyajit Pradhan, Surendra Pratap Mishra, Sunil Choudhary, Chandan Bhai Patel, Saurav Singla, Ashish, Ranjan Kumar Singh
Summary: The study analyzed the spectra of blood serum samples from cervical cancer patients and controls at different treatment phases, showing distinct changes and differences between the two groups. While sensitivity and specificity decreased between different treatment phases, all accuracies of detection and classification remained above 50%. This method can be considered as a screening tool for detecting and monitoring the treatment of cervical cancer.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Neha Kumari, V. L. Mathe, C. Murali Krishna, P. M. Dongre
Summary: The study demonstrated the potential of using BSA conjugates with CIS and GEM anticancer drugs for targeted drug delivery based on different glycations of gp60 protein; UV-visible absorption and fluorescence spectra showed stronger interaction and binding of conjugate 2 and 3 with NANA; Fluorescence quenching data and Raman signal suggested enhanced binding strength of these conjugates with NANA and FUC.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Cell Biology
Rahul Mojidra, Arti Hole, Keita Iwasaki, Hemanth Noothalapati, Tatsuyuki Yamamoto, Murali C. Krishna, Rukmini Govekar
Summary: Monitoring resistance to imatinib in CML patients, especially in resource-poor countries, is essential. Raman spectroscopy shows potential for sensing resistant phenotypes, offering a cost-effective screening tool. This study presents a proof of principle for a single assay for monitoring imatinib resistance.
Article
Nanoscience & Nanotechnology
Sumit K. Mishra, Arti Hole, Pradeep K. Reddy, Rohit Srivastava, Murali Krishna Chilakapati, Abhijit De
Summary: This study demonstrates the utility of Raman-microspectroscopy in fine-tuning safety parameters and precision for photothermal-therapy mediated tumor ablation, showing distinct spectral clusters/zones in treated and untreated tissue areas and providing insight into the molecular/biochemical response in different zones.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2021)
Article
Instruments & Instrumentation
Dimple Saikia, Priyanka Jadhav, Arti R. Hole, Chilakapati Murali Krishna, Surya P. Singh
Summary: This study demonstrates the application of Raman spectroscopy in monitoring the growth kinetics of three different pathogenic bacteria. The spectral features revealed temporal changes in the peptide and nucleic acid content of cells during different growth stages, and successful discrimination between growth phases was achieved using principal component analysis.
APPLIED SPECTROSCOPY
(2022)
Article
Spectroscopy
Priyanka A. Jadhav, Kshama Pansare, Arti Hole, Arvind Ingle, Rukmini Govekar, C. Murali Krishna
Summary: About two thirds of oral cancer cases are diagnosed at advanced stages, highlighting the importance of early screening. This study used an animal model to investigate early biochemical changes during DMBA-induced oral carcinogenesis, and found that Raman spectroscopy can be an effective tool for early diagnosis. The findings suggest that serum RS can detect increasing protein and loss of lipid content as oral carcinogenesis progresses.
JOURNAL OF RAMAN SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Dimple Saikia, Priyanka Jadhav, Arti R. Hole, Chilakapati Murali Krishna, Surya P. Singh
Summary: This study applied Raman spectroscopy to investigate the differences between colistin sensitive and resistant bacterial strains, and successfully generated colistin resistant clones. A unique spectral profile associated with specific drug concentration was obtained, allowing for successful differentiation between resistant and sensitive cells. These findings support the prospective utility of Raman spectroscopy in identifying antimicrobial resistance.
Article
Chemistry, Multidisciplinary
Sukanya Rauniyar, Kshama Pansare, Asmita Sharda, Saurav Raj Singh, Panchali Saha, Murali Krishna Chilakapati, Sanjay Gupta
Summary: Investigating the effect of sub-culturing in parental and radioresistant MCF-7 cells, this study found that radioresistant cells showed high-intensity nucleic acid and cytochrome bands, potentially serving as a radiation-induced spectral marker. Raman spectroscopy data revealed dynamic biochemical alterations in revived radioresistant G(2)/M synchronized cells at early cell passages, stabilizing at a later stage. The importance of cell passaging and cell cycle phases in potentially altering biochemical parameters in radioresistant cells post-cryopreservation was highlighted.
Article
Otorhinolaryngology
Panchali Saha, Sharada Sawant, Atul Deshmukh, Arti Hole, C. Murali Krishna
Summary: This study evaluates the potential of serum Raman spectroscopy (SRS) in identifying recurrence-prone oral cancer subjects. DNA and protein alterations were observed in serum after surgery for all groups. Stratification of post-surgery, recurrence, and non-recurrence subjects was achieved with high accuracy (84%) using SRS, while no stratification was observed before surgery.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2023)