Journal
PHOTOCHEMISTRY AND PHOTOBIOLOGY
Volume 95, Issue 2, Pages 581-586Publisher
WILEY
DOI: 10.1111/php.13028
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Funding
- FDA HHS Funding Source: Medline
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3D bioprinting often involves human mesenchymal stem cells (hMSC) that are differentiated into the desired cells to replace body parts like ears. Scaffolds of crosslinked hydrogels offer structural support during differentiation. Different photoinitiators are used to make free radicals that photocrosslink these hydrogels; the more penetrating ultraviolet A1 (UVA1) (340-400 nm) wavelengths can be used because Irgacure 2959 only absorbs in the UV (100-400 nm) region. We questioned if the L929 mouse fibroblast cells used in the American Society for Testing Materials standard cytotoxicity assays (F895&F813) can predict the viability of hMSC after exposure to UVA1 radiation alone and in combination with Irgacure 2959 (0.05-0.5% w/v usual range). We exposed both cell types to a high dose of LED UVA1 (370 +/- 5 nm; 788 kJ m(-2)) and side by side to increasing UVA1 doses from a glass-filtered black light source combined with either 0.05% (w/v) or 0.5% (w/v) of Irgacure 2959 and monitored their viabilities using flow cytometry. We found UVA1 radiation alone killed similar to 50% of the hMSC cells compared to similar to 8% of the L929 cells and significantly more hMSC than L929 died after UVA1 with Irgacure 2959. Thus, L929 cannot be used to accurately predict the viability of hMSC after these specific 3D bioprinting conditions.
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