Editorial Material
Endocrinology & Metabolism
Zhongjie Fu, Lois E. H. Smith
Summary: Pathologic angiogenesis in eye diseases leading to blindness can be attributed to cell senescence, as demonstrated by the bioinformatics study conducted by Crespo-Garcia, Tsuruda, and Dejda. Their findings were validated in human and mouse retina models, where clearance of senescent cells effectively suppressed neovessel growth.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Daniela F. Santos, Mariana Pais, Claudia N. Santos, Gabriela A. Silva
Summary: Studies have shown that the pyrogallol-O-sulfate metabolite can reduce the expression of pro-angiogenic VEGF in diabetic retinopathy, serving as a potential preventive approach against disease progression. Additionally, it also decreases the expression of inflammatory marker Iba1, highlighting its dual role in targeting molecules involved in both inflammation and neovascularization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Lyndsay L. Leach, Nicholas J. Hanovice, Stephanie M. George, Ana E. Gabriel, Jeffrey M. Gross
Summary: Research has revealed that zebrafish RPE has strong intrinsic regenerative potential, with elements of immune response identified as critical mediators in the regeneration process. Macrophage/microglia cells are responsive to RPE damage and essential for the timely progression of regenerative response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Vicente Bermudez, Paula Estefania Tenconi, Maria Sol Echevarria, Aram Asatrian, Jorgelina Muriel Calandria, Norma Maria Giusto, Nicolas Guillermo Bazan, Melina Valeria Mateos
Summary: This study found that PLD1i and PLD2i can prevent the effects of high glucose concentrations on the phagocytic function of RPE cells, without affecting cell function and viability under non-inflammatory conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
R. Scott Duncan, Andrew Keightley, Adam A. A. Lopez, Conner W. W. Hall, Peter Koulen
Summary: Age-related macular degeneration (AMD) is a common cause of blindness in the elderly, and oxidative stress plays a role in its development. In this study, the researchers used hTERT-RPE cells to understand the pathophysiological changes in RPE during oxidative stress. They found that alpha Toc and gamma Toc independently induced the expression of antioxidant proteins in RPE cells, suggesting potential therapeutic strategies for protecting RPE cells from oxidative stress. Proteomics approaches were used to identify the changes in protein expression in cellular antioxidant response pathways during oxidative stress and after tocopherol treatment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Debora Napoli, Martina Biagioni, Federico Billeri, Beatrice Di Marco, Noemi Orsini, Elena Novelli, Enrica Strettoi
Summary: The study reveals structural abnormalities in the RPE of RP patients, leading to dysfunction of the inner barrier. Findings also suggest a correlation between the extent of photoreceptor loss and interruption zones of ZO-1. Treatment with dexamethasone in RP mice not only rescues cones but also restores RPE structure, highlighting a potential feedback loop for simultaneous protection of cones and the RPE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Tanjina Akter, Balasubramaniam Annamalai, Elisabeth Obert, Kit N. Simpson, Baerbel Rohrer
Summary: This study investigates the potential role of the thrombin inhibitor dabigatran in age-related macular degeneration (AMD). The results show that dabigatran users have a reduced risk of wet AMD diagnosis. Thrombin affects barrier function and downstream signaling, including the expression of connective tissue growth factor (CTGF) and secretion of vascular endothelial growth factor (VEGF), suggesting dabigatran as a potential novel treatment option in AMD.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Ophthalmology
M. Helena Cardoso, Michael J. Hall, Thomas Burgoyne, Pedro Fale, Tina Storm, Cristina Escrevente, Pedro Antas, Miguel C. Seabra, Clare E. Futter
Summary: This study aimed to model the effects of chloroquine on the retinal pigment epithelium in vivo and investigate the impact of mild chloroquine treatment on lysosome function and turnover. The research found that low-dose chloroquine treatment inhibits lysosome reformation, leading to a gradual depletion of lysosomes' ability to interact with cargo-carrying vacuoles and degrade their content. The accumulation of pro-cathepsin D and LAMP1 reflects the inhibition of normal turnover of lysosomal constituents and possibly lysosomes themselves. Understanding the mechanisms underlying lysosome reformation may contribute to the development of new treatments for chloroquine-induced retinopathy.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Hui Peng, Prerana Ramadurgum, DaNae R. Woodard, Steffi Daniel, Emi Nakahara, Marian Renwick, Bogale Aredo, Shyamtanu Datta, Bo Chen, Rafael Ufret-Vincenty, John D. Hulleman
Summary: The DHFR DD system can be effectively applied in various mouse models of ocular diseases, providing a foundation for the conditional control of gene therapy to treat multiple eye diseases.
Review
Pharmacology & Pharmacy
Song Yang, Jun Zhou, Dengwen Li
Summary: The retinal pigment epithelium is crucial for visual functions, but damage to its structure and function can lead to various retinopathies with no cure currently available. Understanding the development, function, and pathobiology of the retinal pigment epithelium is essential for preventing and treating retinopathies. This review discusses potential strategies for treatment and provides new perspectives for future research in this field.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jongmin Kim, Yeo Jin Lee, Jae Yon Won
Summary: This review highlights the roles and pathogenesis of retinal pigment epithelium (RPE) in AMD, emphasizing the importance of understanding the etiology of AMD and discussing potential treatment strategies targeting RPE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Fan-Li Lin, Yu-Wen Cheng, Li-Huei Chen, Jau-Der Ho, Jing-Lun Yen, Mong-Heng Wang, Tzong-Huei Lee, George Hsiao
Summary: Age-related macular degeneration (AMD) is a leading cause of vision loss and blindness without a cure. A natural compound called theissenolactone B (LB53) isolated from Theissenia cinerea has been found to have neuroprotective effects in a mouse model of AMD by inhibiting retinal pigment epithelium-mediated MMP-9 activation and anti-microglia.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Geriatrics & Gerontology
Nan Su, Uwe Hansen, Tanja Plagemann, Karin Gaeher, M. Dominik Leclaire, Jeannette Konig, Annika Hoehn, Tilman Grune, Constantin E. Uhlig, Nicole Eter, Peter Heiduschka
Summary: LF injection into the subretinal space of mice leads to activation and migration of microglial cells, increased expression of inflammatory cytokines, and gradual degradation of RPE cells. These features are consistent with those found in an aging retina, suggesting that LF injection could serve as a model for intermediate and late AMD.
Article
Materials Science, Multidisciplinary
Biola F. Egbowon, Enzo Fornari, Joseph M. Pally, Alan J. Hargreaves, Bob Stevens, T. Martin Mcginnity, Barbara K. Pierscionek
Summary: This study demonstrates that human RPE cell lines (ARPE-19) can be cultured on ultrathin suspended electrospun nanofibre scaffolds (ENS) composed of hydrophobic polymer polyacrylonitrile (PAN) and a water-soluble aliphatic diamine, without or with fluocinolone acetonide (FA) treatment. The cells survived and maintained their morphology for up to 150 days with FA-treated ENS, showing an epithelial phenotype with biomarkers critical for retinal characteristics. This technique has potential for subretinal transplantation.
MATERIALS & DESIGN
(2023)
Article
Biochemistry & Molecular Biology
Jongmin Kim, Jae Yon Won
Summary: As the world ages, the number of age-related diseases, particularly those related to retinal pigment epithelium (RPE) degeneration, has increased. Photobiomodulation (PBM), a non-invasive treatment using red and near-infrared light, has shown potential as a new treatment for ocular diseases. Recent studies have demonstrated its antioxidant effect, making it a promising approach to cure RPE degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Srividya Ganapathy, Hanka Venselaar, Que Chen, Huub J. M. de Groot, Klaas J. Hellingwerf, Willem J. de Grip
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Article
Biochemistry & Molecular Biology
Que Chen, Jos Arents, Srividya Ganapathy, Willem J. de Grip, Klaas J. Hellingwerf
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Que Chen, Jeroen B. van der Steen, Jos C. Arents, Aloysius F. Hartog, Srividya Ganapathy, Willem J. de Grip, Klaas J. Hellingwerf
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Chemistry, Physical
Yusaku Hontani, Srividya Ganapathy, Sean Frehan, Miroslav Kloz, Willem J. de Grip, John T. M. Kennis
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2018)
Article
Multidisciplinary Sciences
Gaoxiang Mei, Natalia Mamaeva, Srividya Ganapathy, Peng Wang, Willem J. DeGrip, Kenneth J. Rothschild
Article
Biotechnology & Applied Microbiology
Que Chen, Jos Arents, J. Merijn Schuurmans, Srividya Ganapathy, Willem J. de Grip, Otilia Cheregi, Christiane Funk, Filipe Branco dos Santos, Klaas J. Hellingwerf
METABOLIC ENGINEERING
(2019)
Article
Biochemistry & Molecular Biology
Srividya Ganapathy, Svenja Kratz, Que Chen, Klaas J. Hellingwerf, Huub J. M. de Groot, Kenneth J. Rothschild, Willem J. de Grip
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2019)
Article
Chemistry, Physical
Yusaku Hontani, Srividya Ganapathy, Sean Frehan, Miroslav Kloz, Willem J. de Grip, John T. M. Kennis
JOURNAL OF PHYSICAL CHEMISTRY B
(2019)
Article
Chemistry, Physical
Hyeon Jeong Lee, Kai-Chih Huang, Gaoxiang Mei, Cheng Zong, Natalia Mamaeva, Willem J. DeGrip, Kenneth J. Rothschild, Ji-Xin Cheng
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2019)
Article
Biochemistry & Molecular Biology
Gaoxiang Mei, Natalia Mamaeva, Srividya Ganapathy, Peng Wang, Willem J. DeGrip, Kenneth J. Rothschild
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Gaoxiang Mei, Cesar M. Cavini, Natalia Mamaeva, Peng Wang, Willem J. DeGrip, Kenneth J. Rothschild
Summary: Opsin-based transmembrane voltage sensors (OTVSs) are widely used in optogenetic applications to measure voltage changes. In this study, QuasAr2 and NovArch were characterized using various spectroscopic techniques, revealing the ability to optically switch between different states, with NovArch showing a previously unknown complexity in its photophysical properties.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Que Chen, Jos Arents, J. Merijn Schuurmans, Srividya Ganapathy, Willem J. de Grip, Otilia Cheregi, Christiane Funk, Filipe Branco dos Santos, Klaas J. Hellingwerf
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Adrian Yi, Hai Li, Natalia Mamaeva, Roberto E. Fernandez De Cordoba, Johan Lugtenburg, Willem J. DeGrip, John L. Spudich, Kenneth J. Rothschild
Article
Biochemistry & Molecular Biology
Petra H. M. Bovee-Geurts, Johan Lugtenburg, Willem J. DeGrip
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2017)
Article
Chemistry, Physical
Andreas Brinkmann, Ulrich Sternberg, Petra H. M. Bovee-Geurts, Isabelle Fernandez Fernandez, Johan Lugtenburg, Arno P. M. Kentgens, Willem J. DeGrip
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2018)