Article
Chemistry, Multidisciplinary
Xuebo Zhang, Daoming Guan, Yawei Liu, Jinyang Liu, Kuangshi Sun, Song Chen, Yanxin Zhang, Bingjie Zhao, Tianli Zhai, Yunxiang Zhang, Fuyou Li, Qian Liu
Summary: Single molecule localization microscopy based on photoactivation has been developed as a powerful tool for investigating the ultrastructure of cells. A general strategy using the 2,3-dihydro-1,4-oxathiine group (SO) as a tag to attach to various skeletal structures was developed. The fluorophores generated singlet oxygen under excitation light, converting the SO moiety into its ester derivative and restoring fluorescence. This technique was successfully applied for time-lapse super-resolution tracking in living cells and super-resolution imaging of microtubule structures in neurons.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Ayse Aktalay, Richard Lincoln, Lukas Heynck, Maria Augusta do R. B. F. Lima, Alexey N. Butkevich, Mariano L. Bossi, Stefan W. Hell
Summary: Highly compact, click compatible, and photoactivatable dyes have been developed for super-resolution fluorescence microscopy. By combining the photoactivatable xanthone (PaX) core with a tetrazine group, minimally sized and highly sensitive molecular dyads were achieved for selective labeling of unnatural amino acids. The quenching properties of tetrazine were exploited to attenuate the photoactivation rates of PaX and reduce the overall fluorescence emission, providing selectivity to reduce off-target signal. These dyads were used in MINFLUX nanoscopy to demonstrate molecular-scale precision in fluorophore positioning.
ACS CENTRAL SCIENCE
(2023)
Review
Chemistry, Multidisciplinary
Yang Zhang, Yeting Zheng, Yasniel Meana, Francisco M. Raymo
Summary: The borondipyrromethene (BODIPY) chromophore is a versatile platform for constructing photoresponsive dyes with unique properties, allowing for spatiotemporal control of fluorescence and implementation of imaging strategies that are impossible with conventional fluorophores.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Alexey N. Butkevich, Michael Weber, Angel R. Cereceda Delgado, Lynn M. Ostersehlt, Elisa D'Este, Stefan W. Hell
Summary: The study introduces a series of fluorescent dyes with hydrophilic carbamate caging groups that can rapidly photoactivate under UV irradiation and are not affected by high-intensity light of double their wavelength. These dyes have high water solubility and transform into validated super-resolution and live-cell-compatible fluorophores upon photoactivation. Using popular fluorescent markers, multiple color images can be obtained with stimulated emission depletion nanoscopy, while precise localization of individual fluorophores can be achieved with a standard deviation of <3 nm using MINSTED and MINFLUX techniques.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Richard Lincoln, Mariano L. Bossi, Michael Remmel, Elisa D'Este, Alexey N. Butkevich, Stefan W. Hell
Summary: Researchers have developed a method to transform 3,6-diaminoxanthones into caging-group-free photoactivatable fluorophores, which assemble into highly fluorescent dyes upon light irradiation. These versatile dyes show potential for various applications in microscopy.
Review
Biochemistry & Molecular Biology
Yang Zhang, Yeting Zheng, Andrea Tomassini, Ambarish Kumar Singh, Francisco M. Raymo
Summary: PALM uses fluorescence photoactivation and single-molecule localization to overcome optical diffraction and achieve nanoscale spatial resolution in imaging biological samples. Synthetic modifications of the BODIPY chromophore allow for photoactivation and precise localization of single molecules. In combination with targeting ligands, photoactivatable BODIPYs can label subcellular components and enable sub-diffraction imaging of live cells using PALM, making them valuable probes for bioimaging applications.
Article
Biochemistry & Molecular Biology
Mateusz Korzec, Sonia Kotowicz, Katarzyna Malarz, Anna Mrozek-Wilczkiewicz
Summary: This paper presents the photophysical and biological properties of eight 3-imino-1,8-naphthalimides. It was found that the tested compounds exhibited strong interactions with DMSO, causing a shift in the emission maximum. The compounds also underwent hydrolysis in acidic environments and their photophysical properties were influenced by the substituents on the imine bonds. Biological tests showed that the compounds exhibited green fluorescence and co-localization in cells, indicating their potential as cellular dyes.
Article
Chemistry, Analytical
Yeeun Roh, Sang-Hun Lee, Jisung Kwak, Hyun Seok Song, Seulgi Shin, Yun Kyung Kim, Jeong Weon Wu, Byeong-Kwon Ju, Boyoung Kang, Minah Seo
Summary: This report introduces a highly improved THz imaging technology by comparing complementary metamaterials based on Babinet's principle, showing significant potential applications in producing clear contrast images in biological samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Multidisciplinary
Sam Benson, Fabio de Moliner, William Tipping, Marc Vendrell
Summary: Recent progress in optical bioimaging has led to the demand for minimal chemical reporters that can preserve the properties and activities of biomolecules. Various approaches have been developed to reduce the size of fluorescent and Raman labels to a few atoms and incorporate them into building blocks to form native-like supramolecular structures. This integration of small optical reporters into biomolecules has enabled the creation of previously inaccessible smart molecular entities, greatly expanding their applications in biological imaging.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Medicinal
Anna St Lorenz, Emmanuel Ramsey Buabeng, Oleh Taratula, Olena Taratula, Maged Henary
Summary: A library of near-infrared heptamethine cyanine dyes has been synthesized and incorporated into polymer-based nanoparticles for biomedical applications. The study focuses on the structure-function relationship of the dyes, emphasizing their photoacoustic and photothermal properties to develop customizable tools for clinical use in the NIR spectral region.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Zhe Li, Ping-Zhao Liang, Tian-Bing Ren, Lin Yuan, Xiao-Bing Zhang
Summary: Self-assembled functionalized organic fluorophores have been developed to overcome the limitations of traditional organic fluorophores, such as low signal-to-background ratio and spurious positive/negative signals. These fluorophores can create nanoaggregates through a self-assembly process, prolonging their residency time in cells and in vivo settings. This emerging field shows significant progress and challenges, and this review summarizes the development history, self-assembly mechanisms, and biomedical applications of self-assembled fluorophores.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Vincent Grenier, Kayli N. Martinez, Brittany R. Benlian, Derek M. Garcia-Almedina, Benjamin K. Raliski, Steven C. Boggess, Johnathan C. Maza, Samantha J. Yang, Anneliese M. M. Gest, Evan W. Miller
Summary: This study demonstrates a molecular prosthetic approach that can substantially prolong imaging in neurons and cardiomyocytes.
ACS CENTRAL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Xiujie Zhao, Hongjie Zhao, Shuo Wang, Zhiwen Fan, Yan Ma, Yongmei Yin, Wei Wang, Rimo Xi, Meng Meng
Summary: Utilizing a twisted structure of TPE to enhance the photothermal conversion efficiency of IR780, the obtained molecule T780T shows strong NIR fluorescence and improved PCE. T780T exhibits excellent photothermal stability and ROS generation capability in the dispersed state, and can be formulated into uniform nanoaggregates in aqueous phase for potential clinical applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Zhicheng Yang, Xiaoxiao Fan, Xianglong Liu, Yanmeng Chu, Zhiyun Zhang, Yue Hu, Hui Lin, Jun Qian, Jianli Hua
Summary: In this study, three novel donor-acceptor-donor type second near-infrared AIE fluorophores based on strong electron-acceptor have been successfully developed for bioimaging. These fluorophores exhibit good photostability and high resolution for mouse ear vessel imaging after encapsulation in nanoparticles with good biocompatibility.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Wen Zhou, Xiaowen He, Jinghui Wang, Shasha He, Chen Xie, Quli Fan, Kanyi Pu
Summary: The article discussed the advantages and disadvantages of immunotherapy and phototherapy, as well as the advantages of phototherapy in improving treatment specificity. Furthermore, it summarized the applications of semiconducting polymer nanoparticles in immunotherapy and immunoactivation imaging.
