Article
Engineering, Environmental
Guofang Xu, Ning Zhang, Xuejie Zhao, Chen Chen, Chunfang Zhang, Jianzhong He
Summary: This study explores the potential for offshore marine microbiota to transform organohalide pollutants and finds that marine Dehalococcoidia have a versatile dehalogenation capacity. The study demonstrates dechlorination of diverse organohalides by offshore marine microbiota, including polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), tetrabromobisphenol A (TBBPA), and 2,4,6-trichlorophenol (2,4,6-TCP). It also identifies the presence of Dehalococcoides and uncultivated Dehalococcoidia in initial sediment microcosms, with Dehalococcoides being dominant in all enrichment cultures.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Engineering, Environmental
Xiuying Li, Yi Yang, Jingjing Wang, Huijuan Jin, Yaozhi Zhang, Yiru Cui, Yufang Song, Jun Yan
Summary: This study identified a anaerobic enrichment culture derived from contaminated river sediment that can mediate the reductive dechlorination of Diclofenac (DCF). It also discovered a new Dehalogenimonas population and highlighted the relevant roles of organohalide-respiring bacteria in the natural attenuation of halogenated contaminants.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Review
Microbiology
Mohammad Sufian Bin Hudari, Hans Richnow, Carsten Vogt, Ivonne Nijenhuis
Summary: This article summarizes the current knowledge on reductive dehalogenation activities of chlorinated ethenes at different temperatures, including the activity and dehalogenation extent in laboratory isolates and enrichment cultures at different temperatures. It also discusses the indirect effects on biotransformation and the impact of anthropogenic activities related to thermal heat management on groundwater temperature.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Article
Microbiology
Jessica M. Ewald, Jerald L. Schnoor, Timothy E. Mattes
Summary: By studying the microbial communities containing Dehalococcoides, this research reveals the differential abundance of functional genes and provides support for the bioremediation of halogenated organic contaminants.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Review
Engineering, Environmental
Rifeng Wu, Shanquan Wang
Summary: The integration of bioremediation with organohalide-respiring bacteria (OHRB) and sulfate radicals-based oxidation (Bio-RD-PAO) shows promising results in addressing organohalide pollution, particularly for lowly-halogenated organohalides. This approach offers advantages such as complete attenuation, high reactivity, and non-selectivity to organohalides, making it a potential solution for future remediation applications.
FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
(2022)
Article
Environmental Sciences
Masaki Asai, Naoko Yoshida, Toshiya Kusakabe, Mohamed Ismaeil, Takumi Nishiuchi, Arata Katayama
Summary: This study presents the isolation and identification of a novel strain of Dehalococcoides mccartyi, NIT01, which is capable of completely dechlorinating trichloroethene to ethene. The genomic analysis and characterization of RdhA enzymes provide insights into the molecular mechanism of dechlorination.
ENVIRONMENTAL RESEARCH
(2022)
Article
Environmental Sciences
Martina Bertolini, Sarah Zecchin, Giovanni Pietro Beretta, Patrizia De Nisi, Laura Ferrari, Lucia Cavalca
Summary: The study evaluated a bioremediation approach for decontaminating an aquifer affected by organohalides from an industrial landfill. Results showed that the addition of a reducing substrate could stimulate natural organohalide respiration, leading to degradation of the contaminants. Field tests demonstrated a decrease in chloroethenes concentration, suggesting the potential for further improvement of the approach.
Article
Engineering, Environmental
Hongyu Dang, Jessica M. Ewald, Timothy E. Mattes
Summary: This research used genome-resolved metagenomic and metatranscriptomic analyses to investigate the ecology of dehalogenating microbial communities, revealing interactions and support between Dehalococcoides and other microorganisms.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Guofang Xu, Siyan Zhao, Chen Chen, Xuejie Zhao, Rajaganesan Ramaswamy, Jianzhong He
Summary: This study reports the reductive dehalogenation of penta-BDEs and PCBs by Dehalococcoides mccartyi strain MB. The strain was able to efficiently debrominate penta-BDEs and dechlorinate PCBs through reductive dehalogenation. The research also revealed the involvement of MbrA in the dehalogenation process and explored the mechanism through proteomic profiling and transcriptional analysis.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Microbiology
Franziska Greiner-Haas, Martin von Bergen, Gary Sawers, Ute Lechner, Dominique Turkowsky
Summary: The strictly anaerobic bacterium Dehalococcoides mccartyi relies on organohalide respiration for energy conservation and growth. Acetylation is used by this bacterium to modulate and maintain protein levels and functionality during growth. The increase in acetylated proteins during growth may contribute to maintaining the organohalide-respiring capacity of the bacterium during the stationary phase.
Article
Engineering, Environmental
Qihong Lu, Jinting Liu, Haozheng He, Zhiwei Liang, Rongliang Qiu, Shanquan Wang
Summary: The addition of waste activated sludge can significantly increase the dechlorination rate of organic halides, providing a growth niche for organohalide-respiring bacteria. It plays a key role in PCE dechlorination and has potential applications in bioremediation.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Engineering, Environmental
Di Cao, Xueqi Chen, Jun Nan, Aijie Wang, Zhiling Li
Summary: In this study, the influences of heavy metal exposure on the organohalide respiration process were investigated using a non-obligate organohalide respiring bacterium. The inhibitory effects and molecular mechanisms of five commonly existed heavy metal ions were thoroughly explored. The findings provide theoretical support for alleviating heavy metal inhibition on organohalide respiration activity in groundwater co-contaminated with organohalides and heavy metals.
Article
Engineering, Environmental
Rifeng Wu, Rui Shen, Zhiwei Liang, Shengzhi Zheng, Yong Yang, Qihong Lu, Lorenz Adrian, Shanquan Wang
Summary: Strategies based on ecological principles can enhance the colonization of OHRB in niches, thereby improving the bioremediation efficiency of chloroethene-contaminated sites.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Physical
Hejie Qin, Tanjie Zha, Kun Qian, Yuankui Sun, Xiaohong Guan, Chuncheng Chen
Summary: By embedding enzyme-like Co-N4 sites in a nitrogen-doped carbon support, we developed a Co-NC catalyst that combines the advantages of abiotic and biological dechlorination strategies. Co-NC exhibited excellent reactivity and stability for catalytic dechlorination of chlorinated ethenes. It could transform up to 94.8% of trichloroethene directly to acetylene without toxic intermediates and utilize electrons from dissolved Fe(II), making it a sustainable remediation strategy.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2023)
Article
Microbiology
Yi Yang, Torsten Schubert, Yan Lv, Xiuying Li, Jun Yan
Summary: This study conducted phylogenetic and pangenome analyses of Sulfurospirillum genomes to understand their evolutionary and metabolic potentials. The results revealed conserved regions in the genomes of organohalide-respiring Sulfurospirillum strains that are involved in the organohalide respiration process. Additionally, various Sulfurospirillum species strains were found to have conserved metabolisms and biosynthesis capabilities. This study contributes to the understanding and potential applications of Sulfurospirillum in bioremediation of organohalide pollutants in low-pH environments.
Article
Toxicology
Janneke Elzinga, Menno Grouls, Guido J. E. J. Hooiveld, Meike van der Zande, Hauke Smidt, Hans Bouwmeester
Summary: There is a need for standardized in vitro models of the human intestinal tract to study intestinal health without using animals. The Caco-2 cell line is a widely accepted and characterized model for studying intestinal drug transport, host-microbe interactions, and toxicity. We investigated the effect of different experimental variables on gene expression using transcriptome analysis of Caco-2 cells grown on permeable membranes. Our analysis revealed a weak association between experimental variables and gene expression, possibly due to heterogeneity in cell culture practice and inadequate reporting. This highlights the need for improved reporting protocols and serves as a template for future comparative studies.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Environmental Sciences
Wei-Ying Ouyang, Steffen Kuemmel, Lorenz Adrian, Yong-Guan Zhu, Hans H. Richnow
Summary: This study analyzed compound specific isotope fractionation of the antibiotic sulfamethoxazole (SMX) during anaerobic transformation using the model organism Desulfovibrio vulgaris Hildenborough. The results showed significant carbon and hydrogen isotopic fractionation due to the rearrangement of the isoxazole ring in SMX. Carbon isotope fractionation was higher than values reported for aerobic degradation or abiotic reactions, indicating its potential as a parameter to differentiate reaction mechanisms of SMX transformation. The observed apparent kinetic isotope effect for anaerobic transformation was distinct from that reported for microbial aerobic degradation. Dual-element (C-H) isotope analysis of SMX using gas chromatography was also performed in this study.
Article
Engineering, Environmental
Sara N. Jimenez-Volkerink, Joaquim Vila, Maria Jordan, Cristina Minguillon, Hauke Smidt, Magdalena Grifoll
Summary: Polar biotransformation products have been found to cause an increase in genotoxicity after the bioremediation of PAH-contaminated soils. The microorganisms and mechanisms underlying their further biodegradation have not been fully understood.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Ton That Huu Dat, Georg Steinert, Nguyen Thi Kim Cuc, Pham Viet Cuong, Hauke Smidt, Detmer Sipkema
Summary: Recent reviews have shown that sponge-associated bacteria are valuable sources of structurally diverse secondary metabolites with potent biological activities, making these microbial communities promising for new drug candidates. However, accessing the overall diversity of secondary metabolite biosynthetic potential in bacteria is challenging due to the difficulty in culturing the majority of bacteria in the laboratory. This study investigates the diversity of secondary metabolite biosynthetic gene clusters in metagenomes of bacterial communities associated with three different sponge species and identifies numerous potential gene clusters for the production of novel compounds.
Article
Biotechnology & Applied Microbiology
Emea Okorafor Ude, Jucelaine Haas, Mohammed Kaysar Kaiyoum, Chang Ding, Lorenz Adrian
Summary: The effect of environmental stresses on anammox bacteria was investigated using an overnight batch activity test. It was found that oxygen strongly inhibited anammox activity, but this inhibition could be reversed by sodium sulfite. Additionally, antibiotics, activated carbon, and Fe2O3 had positive effects on anammox activity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Pediatrics
Desiree F. Baaleman, Carrie A. M. Wegh, Tessa J. M. de Leeuw, Faridi S. van Etten-Jamaludin, Elaine E. Vaughan, Margriet H. C. Schoterman, Clara Belzer, Hauke Smidt, Merit M. Tabbers, Marc A. Benninga, Ilan J. N. Koppen
Summary: A systematic review was conducted to estimate normal reference values for defecation frequency and stool consistency in healthy children up to age 4. The results showed that younger infants have a higher frequency of defecation compared to young children, and the type of feeding and age also have an impact on defecation frequency and stool consistency.
JOURNAL OF PEDIATRICS
(2023)
Article
Microbiology
Martha F. Endika, David J. M. Barnett, Cynthia E. Klostermann, Henk A. Schols, Ilja C. W. Arts, John Penders, Arjen Nauta, Hauke Smidt, Koen Venema
Summary: Antibiotic exposure disrupts infant gut microbiota, but the ability to recover from this disruption depends on the type of antibiotic. This study investigated the effect of two common infant prebiotics, 2'-fucosyllactose (2'-FL) and galacto-oligosaccharides (GOS), on the resilience of the gut microbiota after exposure to amoxicillin/clavulanate. The results showed that the prebiotics supported the recovery of the microbiota and restored the production of beneficial fatty acids.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Jesica M. Soder-Walz, Kenneth Wasmund, Darja Deobald, Teresa Vicent, Lorenz Adrian, Ernest Marco-Urrea
Summary: This study sequenced the genome of Dehalobacter sp. strain 8M and analyzed its protein expression. It discovered evidence of interactions among respiratory proteins and discussed implications for the respiratory architecture and advances in understanding this unique respiratory chain.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Shangwei Zhang, Wu Wen, Xinghui Xia, Wei Ouyang, Bi-xian Mai, Lorenz Adrian, Gerrit Schuurmann
Summary: Thermodynamics reveals a proton-coupled two-electron transfer mechanism in microbial B-12-dependent reductive dehalogenation of aromatics, enhancing our understanding of the dehalogenation process.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Rifeng Wu, Rui Shen, Zhiwei Liang, Shengzhi Zheng, Yong Yang, Qihong Lu, Lorenz Adrian, Shanquan Wang
Summary: Strategies based on ecological principles can enhance the colonization of OHRB in niches, thereby improving the bioremediation efficiency of chloroethene-contaminated sites.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Microbiology
Angela Sessitsch, Steve Wakelin, Michael Schloter, Emmanuelle Maguin, Tomislav Cernava, Marie-Christine Champomier-Verges, Trevor C. Charles, Paul D. Cotter, Ilario Ferrocino, Aicha Kriaa, Pedro Lebre, Don Cowan, Lene Lange, Seghal Kiran, Lidia Markiewicz, Annelein Meisner, Marta Olivares, Inga Sarand, Bettina Schelkle, Joseph Selvin, Hauke Smidt, Leo van Overbeek, Gabriele Berg, Luca Cocolin, Yolanda Sanz, Wilson Lemos Fernandes, S. J. Liu, Matthew Ryan, Brajesh Singh, Tanja Kostic
Summary: Microbiomes play crucial roles in ecosystem functioning, supporting planetary health through nutrient cycling, climate regulation, and water filtration. They are closely associated with complex multicellular organisms and contribute to the health of their hosts. However, there is still limited understanding of microbiome transfer and connectivity.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Microbiology
Maria L. Bonatelli, Thore Rohwerder, Denny Popp, Yu Liu, Caglar Akay, Carolyn Schultz, Kuan-Po Liao, Chang Ding, Thorsten Reemtsma, Lorenz Adrian, Sabine Kleinsteuber
Summary: In this study, the metabolism of acesulfame by microbial degraders was investigated, leading to the identification of two gene clusters involved in the degradation pathway. Transposition of these gene clusters between chromosome and plasmid was observed. The global evolution and spread of the acesulfame degradation pathway was also elucidated, highlighting the importance of this research in understanding microbial degradation of harmful substances and environmental remediation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Taojun Wang, Nils Leibrock, Caroline M. Plugge, Hauke Smidt, Erwin G. Zoetendal
Summary: Methanogens, reductive acetogens, and sulfate-reducing bacteria interact with each other in gut ecosystems, and their interactions may vary depending on environmental conditions.
Article
Chemistry, Physical
Shangwei Zhang, Wei Ouyang, Xinghui Xia, Wu Wen, Lorenz Adrian, Gerrit Schueuermann
Summary: Microbial reductive dechlorination is an environmentally friendly and highly desirable method for addressing PCB pollution in soil, sediment, and underground water. The reaction is catalyzed by supernucleophilic cob(i)alamin housed in reductive dehalogenases (RDases), but the mechanism is still unknown.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Environmental Sciences
Sara N. Jimenez-Volkerink, Maria Jordan, Hauke Smidt, Cristina Minguillon, Joaquim Vila, Magdalena Grifoll
Summary: By studying a specific microbial consortium in PAH-contaminated soils, it was discovered that the degradation of the carcinogenic compound BaAQ may occur through the Baeyer-Villiger monooxygenase (BVMOs) pathway, and that horizontal gene transfer may play a significant role in this degradation process.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
