Article
Pharmacology & Pharmacy
Pei F. Lai, Roger C. Young, Rachel M. Tribe, Mark R. Johnson
Summary: The study found that the combined treatment of aminophylline and progesterone did not suppress uterine contractions more effectively than using either drug alone, potentially due to the low specificity and efficacy of aminophylline. It is also important to consider the actions of cAMP and progesterone in neighboring reproductive tissues during pregnancy.
PHARMACOLOGY RESEARCH & PERSPECTIVES
(2021)
Article
Cell Biology
Anton J. M. Roks
Summary: This review focuses on the role of phosphodiesterases in regulating the levels of cyclic adenosine monophosphate and cyclic guanosine monophosphate. Specifically, phosphodiesterase 1 is highlighted, discussing its structure, function, and potential as a drug target for cardiovascular modulation. The various isoforms of phosphodiesterase 1 in different cell types and their effects on vasomotor control, cardiac myocyte function, growth control, fibrosis, and aging are explored. The modulation of phosphodiesterase 1 in different physiological conditions and its clinical relevance are summarized, along with the involvement of nanodomains. The potential use of PDE1 inhibitors as clinical drugs for cardiovascular disease is also discussed.
CELLULAR SIGNALLING
(2022)
Article
Biochemical Research Methods
Aleksandra Stec, Monika Targonska, Edyta Karkosinska, Monika Slowik, Agata Ploska, Leszek Kalinowski, Bartosz Wielgomas, Krzysztof Waleron, Jacek Jasiecki, Szymon Dziomba
Summary: The overproduction of proteins in Chinese hamster ovary (CHO) cells can induce the secretion of extracellular vesicles (EVs), potentially leading to the packaging of overexpressed proteins into exosomes. These findings are crucial for the manufacturing of therapeutic drugs in CHO expression systems, as there is a risk of product loss during downstream processing of culture medium and the use of exosomes as nanocarriers for therapeutic proteins.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Cardiac & Cardiovascular Systems
Lingfeng Luo, Yujun Cai, Yishuai Zhang, Chia G. Hsu, Vyacheslav A. Korshunov, Xiaochun Long, Peter A. Knight, Bradford C. Berk, Chen Yan
Summary: The study revealed that PDE10A contributes to SMC proliferation and intimal hyperplasia by antagonizing the CNP/NPR2/cGMP/PKG1alpha signaling pathway, suggesting that PDE10A may be a novel drug target for treating vascular occlusive disease.
CARDIOVASCULAR RESEARCH
(2022)
Article
Multidisciplinary Sciences
Chongyang Zhang, Hongmei Zhao, Yujun Cai, Jian Xiong, Amy Mohan, Danfei Lou, Hangchuan Shi, Yishuai Zhang, Xiaochun Long, Jing Wang, Chen Yan
Summary: Abdominal aortic aneurysm (AAA) is a common vascular pathology in elderly males characterized by aorta dilation, with cyclic nucleotide phosphodiesterase 1C (PDE1C) playing a critical role in its development. PDE1C elevation contributes to SMC senescence and AAA progression, potentially serving as a therapeutic target against aortic aneurysms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biotechnology & Applied Microbiology
Melanie Nguyen, Aline Zimmer
Summary: Chinese hamster ovary (CHO) cells are widely used in the biopharmaceutical industry for producing recombinant proteins. The proteomic studies on CHO cells have provided valuable insights into the protein clusters, regulatory networks, and pathways governing phenotypic changes, which can be utilized to optimize CHO-based bioprocesses.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Pharmacology & Pharmacy
Enio Setsuo Arakaki Pacini, Edwin Kerry Jackson, Rosely Oliveira Godinho
Summary: In the respiratory tract, extracellular 3',5'-cAMP is metabolized by enzymes to produce 5'-AMP, adenosine, and inosine, affecting airway smooth muscle contraction and relaxation. This extracellular enzymatic system plays a role in respiratory physiology and pathophysiology by generating interstitial adenosine from 3',5'-cAMP.
BIOCHEMICAL PHARMACOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Jun-He Zhang, Lin-Lin Shan, Fan Liang, Chen-Yang Du, Jing-Jing Li
Summary: Recombinant antibodies are a rapidly developing class of therapeutic agents, but high-level expression and production of these antibodies remain a major challenge. This review provides a comprehensive overview of various strategies for optimizing gene sequences, expression vectors, host cell lines, and glycosylation modifications to improve the efficiency and quality of recombinant antibody production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Azam Rahimpour, Es'hagh Pourmaleki, Forough Shams, Zahra Payandeh, Navid Pourzardosht, Mojtaba Didehdar, Milad Gholami
Summary: This study constructed a novel vector system using the Ccnb1ip1 gene insulator element for the expression of an anti-CD52 mAb in CHO cells. The expression of mAb in cells created with the insulator-containing vector was found to be increased by 10.9% compared to the control vector.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Chien-Chung Yang, I-Ta Lee, Yan-Jyun Lin, Wen-Bin Wu, Li-Der Hsiao, Chuen-Mao Yang
Summary: This study confirmed that thrombin induces the production of COX-2 and PGE(2) in human tracheal smooth muscle cells, leading to inflammation in the airways and lungs. The study identified specific signaling pathways involved, including the PKC delta/Pyk2/c-Src/EGFR/PI3K/Akt/AP-1 pathway. Resveratrol was also found to inhibit COX-2 and PGE(2) production by blocking the activation of these signaling molecules, suggesting its potential use in managing airway inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Thu M. Cao, Dayue Chen, Gavin C. Barnard, Amy Shen
Summary: This study explores the potential of using CHO cells for rAAV production and identifies cap protein expression as a limiting factor. By increasing cap protein expression, rAAV titers in CHO cells were comparable to those in HEK-derived rAAV, providing a foundation for potential rAAV production in CHO cells.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Eleftheria Skrika-Alexopoulos, C. Mark Smales
Summary: Exosomes were isolated from CHO cells using a novel method and characterized to determine their protein and RNA content. These findings contribute to the future research and improvement of exosome manufacturing from CHO cells.
BIOTECHNOLOGY LETTERS
(2023)
Article
Biotechnology & Applied Microbiology
Ioscani Jimenez del Val, Sarantos Kyriakopoulos, Simone Albrecht, Henning Stockmann, Pauline M. M. Rudd, Karen M. M. Polizzi, Cleo Kontoravdi
Summary: Metabolic modeling is important for characterizing biopharmaceutical cell culture processes. However, current metabolic models of CHO cells face challenges in terms of size and interpretation. To address these challenges, the CHOmpact model was developed as a reduced metabolic network with improved interpretability and physiological consistency. This model provides a platform for developing dynamic metabolic models to optimize biopharmaceutical cell culture processes.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Biotechnology & Applied Microbiology
Weifeng Li, Zhenlin Fan, Yan Lin, Tian-Yun Wang
Summary: This review highlights the importance of serum-free medium (SFM) in the production of recombinant therapeutic proteins (RTPs) using Chinese hamster ovary (CHO) cells, with nearly 70% of RTPs currently produced in this manner. It discusses the history, key components, preparation, and experimental design of SFM, as well as the potential benefits of small molecule compound additives in improving RTP yield and quality. Lastly, it explores the future prospects of using SFM with CHO cells for RTP production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Katja Glinsek, Kristof Bozovicar, Tomaz Bratkovic
Summary: CRISPR technology has made significant progress in CHO cell line engineering, improving protein production and product quality. It allows for glycosylation modulation, productivity enhancement, tackling adventitious agents, elimination of problematic host cell proteins, development of antibiotic-free selection systems, site-specific transgene integration, and CRISPR-mediated gene activation and repression. The review highlights the potential of CRISPR technology in CHO cell line genome editing and epigenetic engineering for more efficient and cost-effective development of biopharmaceuticals, ensuring the safety and quality of the final product.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Manuel Sanchez, Lorena Suarez, Begona Cantabrana, Javier Bordallo
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2017)
Article
Food Science & Technology
Manuel Sanchez, Lorena Suarez, Maria Teresa Andres, Blanca Henar Florez, Javier Bordallo, Sabino Riestra, Begona Cantabrana
FOOD & NUTRITION RESEARCH
(2017)
Article
Gastroenterology & Hepatology
Eva Barreiro-Alonso, Cristina Saro-Gismera, Manuel Sanchez
EXPERT REVIEW OF GASTROENTEROLOGY & HEPATOLOGY
(2018)
Article
Cell Biology
Ines Lopez-Alonso, Jorge Blazquez-Prieto, Laura Amado-Rodriguez, Adrian Gonzalez-Lopez, Aurora Astudillo, Manuel Sanchez, Covadonga Huidobro, Cecilia Lopez-Martinez, Claudia C. dos Santos, Guillermo M. Albaiceta
SCIENCE TRANSLATIONAL MEDICINE
(2018)
Article
Pharmacology & Pharmacy
Manuel Chacon, Natalia Vazquez, Silvia Berisa, Mairobi Persinal, Manuel Sanchez, Begona Baamonde, Jose F. Alfonso, Luis Fernandez-Vega Cueto, Jesus Merayo-Lloves, Alvaro Meana
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2019)
Retraction
Biochemistry & Molecular Biology
Guillermo Marino, Jose A. Uria, Xose S. Puente, Victor Quesada, Javier Bordallo, Carlos Lopez-Otin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Nutrition & Dietetics
Lorena Suarez, Maria Moreno-Luque, Irene Martinez-Ardines, Nereida Gonzalez, Patricia Campo, Palmira Huerta-Cima, Manuel Sanchez
BRITISH JOURNAL OF NUTRITION
(2019)
Article
Dermatology
Cristina Galache-Osuna, Sebastian Reyes-Garcia, Esther Salgueiro, Javier Bordallo-Landa, Ana Lozano, Francisco Vazquez-Lopez, Jorge Santos-Juanes
Summary: This study aimed to evaluate the long-term survival of apremilast and its predictive factors when used to treat psoriasis. The results showed that apremilast may be a suitable and efficient alternative for the treatment of psoriasis patients in the PASI<10 group.
DERMATOLOGIC THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Manuel Chacon, Manuel Sanchez, Natalia Vazquez, Mairobi Persinal-Medina, Sergio Alonso-Alonso, Begona Baamonde, Jose F. Alfonso, Luis Fernandez-Vega-Cueto, Jesus Merayo-Lloves, Alvaro Meena
Summary: Impedance analysis can be used to evaluate cell maturation and detect slight cell damages in RhCE models, providing a faster and simpler method for assessing ocular irritancy with comparable accuracy to current methodologies.
BIOELECTROCHEMISTRY
(2022)
Article
Communication
Maria Gonzalez-Garcia, Begona Cantabrana, Ana Fernandez-Feito, Alberto Lana, Agustin Hidalgo
Summary: The study found that the newspaper El Pais frequently covered topics related to public health and health management from 2001 to 2016, including disease prevention, health promotion, and different levels of management. The articles were mostly informative, came from official sources, and were mainly published in the Society section.
REVISTA ESPANOLA DE COMUNICACION EN SALUD
(2021)
Article
Medicine, General & Internal
Maria Gonzalez Garcia, Begona Cantabrana Plaza, Agustin Hidalgo Balsera
ARCHIVOS DE MEDICINA
(2020)
Meeting Abstract
Pharmacology & Pharmacy
M. Gonzalez, B. Cantabrana, A. Hidalgo
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2018)