Journal
PHARMACOGNOSY MAGAZINE
Volume 10, Issue 37, Pages 77-82Publisher
MEDKNOW PUBLICATIONS & MEDIA PVT LTD
DOI: 10.4103/0973-1296.126671
Keywords
Agrobacterium rhizogenes R1601; anthraquinones; Polygonum multiflorum; transgenic hairy roots
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Funding
- National Natural Sciences Foundation of China [81102779]
- Zhujiang New Star Award for Excellence in Science and Technology of Guangzhou
- Guangdong Natural Science Foundation [9451022401003453]
- Guangdong Medical Research Foundation of Science and Technology [B2011151]
- Undergraduate Innovative Experimental Project of Guangdong Pharmaceutical University
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Background: Polygonum multiFLorum is an important medicinal plant. Hairy roots systems obtained by transforming plant tissues with the natural genetic engineer Agrobacterium rhizogenes can produce valuable biological active substances, which have immense potential in the pharmaceutical industry. Objective: To optimize the inductive and cultural conditions of P. multiFLorum hairy roots and to identify the major active secondary metabolites in hairy roots. Materials and Methods: P. multiFLorum hairy root were mediated with A. rhizogenes R1601 to induce hairy roots. Four combinations, including Murashige-Skoog (MS), 1/2 MS, B 5 , and White, were investigated to optimize the culture medium. MS medium was selected for the growth measurement. The qualitative and quantitative determinations of free anthraquinone in hairy roots were compared with the calli and aseptic plantlets using high-performance liquid chromatography. Results: The inductive rates of hairy roots by leaves were higher than for any other explants. The presence of agropine in the P. multiflorum hairy roots confirmed that they were indeed transgenic. MS medium was the most suitable of the four media for hairy root growth. Meanwhile, the growth kinetics and nutrient consumption results showed that the hairy roots displayed a sigmoidal growth curve and that their optimal inoculation time was 18-21 days. The determination of the anthraquinone constituents indicated that the rhein content of the hairy roots reached 2.495 g g(1) and was 2.55-fold higher than that of natural plants. Conclusion: Transgenic hairy roots of P. multiflorum could be one of the most potent materials for industrial-scale production of bioactive anthraquinone constituents.
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